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Buffered Peptone Water (BPW)
  • Buffered Peptone Water (BPW)
Buffered Peptone Water (BPW)

Buffered Peptone Water (BPW)

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Buffered Peptone Water (BPW) | Non-Selective Pre-Enrichment Medium | AS-1160
AuSaMicS Life Science • Food & Environmental Microbiology

Buffered Peptone Water (BPW)

Non-Selective Pre-Enrichment Medium for Food & Environmental Microbiology

A gentle non-selective pre-enrichment medium formulated for the recovery and resuscitation of stressed or sub-lethally injured microorganisms, especially Salmonella spp., before selective enrichment and plating steps in regulatory and routine microbiology workflows.

AS-1160 🧫 Non-Selective ✓ ISO-Aligned 🧪 Pre-Enrichment
Application: Food, water, environmental microbiology Preparation: 20.0 g/L Incubation: 35–37°C / 18–24 h / aerobic

Pre-Enrichment Specialist

Peptone — gentle nutrient recovery
Phosphate Buffer — stable neutral pH
NaCl — osmotic balance
Non-selective system — broad microbial resuscitation
Essential First Step
Salmonella & injured-cell recovery

Overview

Buffered Peptone Water (BPW) is a non-selective pre-enrichment medium specifically designed for the recovery and resuscitation of stressed or sub-lethally injured microorganisms, particularly Salmonella spp., from food, water, and environmental samples.

BPW is widely accepted and specified in multiple international ISO workflows and plays a critical role as the first enrichment step in many regulatory microbiological testing methods. During food processing, microorganisms may be exposed to heat, acidity, dehydration, oxidative stress, or preservatives that injure cells and reduce direct recovery on selective media.

By offering a gentle nutrient environment with stable buffering, BPW allows injured organisms to repair and recover before exposure to selective enrichment broths and selective agar media, improving sensitivity and method reliability.

Principle of the Medium

Buffered Peptone Water contains peptone, sodium chloride, and a phosphate buffering system. Together, these components create favorable osmotic conditions and maintain a stable near-neutral pH during the early recovery stage.

This buffering capacity minimizes the inhibitory effects of acidic or otherwise stressful food matrices and supports cellular repair and metabolic recovery. Because target organisms such as Salmonella may be present in low numbers and in an injured state, pre-enrichment in BPW is often essential before exposure to selective enrichment media.

In practical workflows, BPW acts as a recovery medium rather than a differential or selective system, making it particularly valuable when maximum resuscitation is more important than early suppression of background flora.

Applications

  • Pre-enrichment of Salmonella spp. from food and water samples
  • Recovery of stressed or injured bacteria following food processing
  • Enumeration and detection workflows involving Listeria monocytogenes
  • Sample dilution and homogenization in microbiological analysis
  • Food safety, environmental, and water microbiology laboratories
  • Routine and regulatory microbiological testing

Complete Formulation & Functional Profile

Indicative Composition (per Liter)

Ingredient Concentration Function Technical Role
Peptone 10.0 g Nutrient source Supports microbial repair and recovery
Sodium chloride 5.0 g Osmotic balance Maintains physiological conditions
Disodium hydrogen phosphate 3.5 g Buffer component Supports stable neutral pH
Potassium dihydrogen phosphate 1.5 g Buffer component Completes phosphate buffering system

Typical final pH: 7.0 ± 0.2 at 25°C. Typical reconstitution: 20.0 g/L.

Recovery Logic

Gentle Nutrition
Promotes repair of stressed or injured cells without selective pressure.
pH Stability
Phosphate buffering reduces inhibitory effects from acidic sample matrices.
Method Compatibility
Acts as the first-stage medium before selective enrichment and plating.
Practical Note: BPW is designed to maximize resuscitation and recovery, not to inhibit background flora at the earliest stage.

Pre-Enrichment Media Comparison

Medium Primary Role Strength Limitation Best Use
Buffered Peptone Water (BPW) Non-selective pre-enrichment Excellent for injured-cell recovery and sample homogenization No early suppression of competitors Salmonella pre-enrichment and broad recovery workflows
Peptone Water Simple nutrient diluent Basic recovery support Less buffered than BPW General microbiological dilution
Lactose Broth Pre-enrichment / presumptive coliform workflows Useful in some traditional methods Not as broadly preferred for modern BPW-type workflows Legacy or method-specific use
Selective enrichment broths Selective recovery after pre-enrichment Suppress background flora Can inhibit injured cells if used too early Second-stage enrichment

Selection Guide

Choose BPW if:
• Cells may be heat-, acid-, or dehydration-stressed
• A non-selective first step is required
• Sample homogenization and recovery are both needed
Choose a selective broth if:
• Pre-enrichment is already complete
• You are moving to target-specific enrichment stages
Choose a simpler diluent if:
• Buffering is not important
• The method does not require injured-cell recovery emphasis

Quality Positioning & Workflow Value

Typical Use:
First-stage recovery medium before selective enrichment
Sample Scope:
Food, water, environmental, and processing-associated samples
Commercial Position:
Core staple medium for regulatory and QC labs
Cross-Workflow Fit:
Salmonella, Listeria, Enterobacteriaceae-related methods
Commercial Positioning: A high-volume, reference-style pre-enrichment medium that laboratories expect to be reliable, economical, and consistent across batches.

Technical Specifications

Cat. No. AS-1160
Medium Type Non-selective pre-enrichment medium
Reconstitution 20.0 g/L
Final pH 7.0 ± 0.2 at 25°C
Appearance Off-white to light beige powder
Solubility Completely soluble in water
Sterilization 121°C for 15 minutes
Incubation 35–37°C / 18–24 hours / aerobic
Storage Dehydrated: 15–30°C; Prepared: 2–8°C
HS Code 3822.00

Preparation Protocol

  1. 1 Suspend 20.0 g of dehydrated medium in 1 L purified water.
  2. 2 Heat with agitation until completely dissolved.
  3. 3 Sterilize by autoclaving at 121°C for 15 minutes.
  4. 4 Cool to room temperature before use.

Pack Sizes & Suggested Pricing

100 g AUD 32
250 g AUD 59
500 g AUD 95
1 kg AUD 165
5 kg AUD 640

Quality & Compliance

  • Manufactured under controlled conditions
  • Batch-to-batch consistency verified by QC testing
  • Conforms to ISO performance requirements
  • Supported by Technical Data Sheet (TDS) and Certificate of Analysis (COA)

Why Buy BPW from AuSaMicS?

  • Australian-based supplier with fast local dispatch
  • ISO-aligned, reference-grade formulation
  • Clear technical documentation and responsive support
  • Competitive pricing and reliable bulk supply

Bulk pricing enquiries: sales@ausamics.com

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AuSaMicS Compliance & Supply Notes

For laboratory, food, and environmental microbiology use only. Not intended for diagnostic or clinical use in humans or animals.

Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.

AuSaMicS Pty Ltd
31 Longview CT, Thomastown, VIC 3074, Australia
ABN 56676640467
www.ausamics.com.au
support@ausamics.com
+61 412 520 598

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