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2,3,5-Triphenyltetrazolium Chloride (TTC, CAS 298-96-4) | Redox & Cell Viability Indicator for Microbiology and Seed Testing
  • 2,3,5-Triphenyltetrazolium Chloride (TTC, CAS 298-96-4) | Redox & Cell Viability Indicator for Microbiology and Seed Testing
  • 2,3,5-Triphenyltetrazolium Chloride (TTC, CAS 298-96-4) | Redox & Cell Viability Indicator for Microbiology and Seed Testing
2,3,5-Triphenyltetrazolium Chloride (CAS 298-96-4) | TTC Laboratory Reagent

2,3,5-Triphenyltetrazolium Chloride (CAS 298-96-4) | TTC Laboratory Reagent

$19.00

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2,3,5-Triphenyltetrazolium Chloride (TTC)

Redox Indicator Dye for Microbiological Enumeration

Catalog Number: ASE-905
CAS Number: 298-96-4
Molecular Formula: C₁₉H₁₅ClN₄
Molecular Weight: 334.80 g/mol

Overview

2,3,5-Triphenyltetrazolium Chloride (TTC) is a redox indicator dye widely used in microbiology for the detection, differentiation, and enumeration of viable microorganisms in solid culture media.

TTC is colourless in its oxidised form and is reduced by active microbial dehydrogenase enzymes to form red, insoluble formazan. This reaction occurs only in metabolically active (living) cells, allowing clear visual distinction between viable and non-viable microorganisms. Colonies or cells that reduce TTC appear red, while dead cells remain colourless.

Ausamics TTC is supplied with consistent purity and performance, suitable for routine microbiological analysis and research applications.

Key Features & Benefits

  • Redox indicator for detecting microbial metabolic activity

  • Produces distinct red formazan in viable cells

  • Enables clear colony visualization and counting

  • Differentiates living vs non-viable microorganisms

  • Widely used in bacteriological and food microbiology media

Typical Applications

Microbiological Enumeration

  • Colony counting in solid culture media

  • Differentiation of viable colonies in mixed populations

  • Enhancement of colony visibility on agar plates

Food, Water & Environmental Microbiology

  • Enumeration of bacteria in food and beverage testing

  • Detection of viable microorganisms in water samples

  • Environmental and quality-control testing

Research & Teaching Laboratories

  • Viability and metabolic activity studies

  • Demonstration of redox reactions in microbiology

  • Microbial physiology and enzyme activity assays

Principle of the Dye

TTC functions as an artificial electron acceptor. In viable cells, intracellular dehydrogenase enzymes reduce TTC to red formazan, which accumulates as intracellular granules. Non-viable cells lack enzymatic activity and therefore do not reduce TTC, remaining colourless. This reaction provides a simple and reliable visual indicator of microbial viability.

Physical & Chemical Properties

  • Appearance: White to pale crystalline powder

  • Solubility: Soluble in water

  • Colour change: Colourless → red (upon reduction)

  • Light sensitivity: Sensitive to light; store protected

Storage & Stability

  • Store at 15–25 °C in a cool, dry place

  • Protect from light and moisture

  • Keep container tightly closed

  • Use before the stated expiry date

Intended Use

For laboratory research use only.
Not for human or animal use.
Not for food, feed, or pharmaceutical applications.

Quality & Compliance

  • Suitable for microbiological and research applications

  • Supplied with Certificate of Analysis (COA)

  • SDS available upon request

  • Consistent batch-to-batch performance

Customs & Trade Information

HS / AHECC Code: 3204.19.00
Synthetic organic colouring matter and preparations

Why Buy TTC from Ausamics?

Ausamics is an Australian life-science supplier specialising in microbiological reagents and dyes, offering:

  • Reliable local supply

  • Consistent quality and documentation

  • Competitive pricing

  • Responsive technical support

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