Skip to product information
Bulk Order
Request a Quote
Acriflavine HCl Powder | ASA-2037
$27.00 AUD
Documentation
AuSaMicS Life Science · Research Biochemical
Acriflavine Hydrochloride — Acridine Dye
Fluorescent Nucleic Acid Stain for Microbiology & Molecular Research
Bright orange acridine dye with strong DNA/RNA binding affinity. Used for fluorescent staining, intercalation studies, and biomedical research models.
ASA-2037 CAS 8063-24-9 ≥90% Purity (HPLC) 🇦🇺 Melbourne Stock ⚡ Same-Week Dispatch ⚠ GHS Danger
Purity
≥90%
HPLC assay
Loss on Drying
≤6.5%
at 105°C
Appearance
Orange
crystalline powder
Solubility
Soluble
water & ethanol
📄 Full DocumentationCOA · TDS · SDS every batch
🇦🇺 Local Australian StockNo import lead times
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportFrom our science team
Overview
Acriflavine Hydrochloride (ASA-2037) is a synthetic acridine dye derivative with a long-established role in microbiology, molecular biology, and biomedical research. Its bright orange colour and strong binding affinity to nucleic acids underpin its use as a fluorescent staining agent and research-grade antimicrobial compound.
Historically used as an antiseptic dye, acriflavine has more recently drawn interest in oncology and antiviral research, where it is investigated as a biologically active small molecule under controlled laboratory conditions. Today it is widely used in research laboratories, aquaculture research studies, and microbiological investigations. AuSaMicS Acriflavine Hydrochloride is supplied for laboratory and research use, with controlled quality and full batch documentation.
Principle of Activity
Acriflavine Hydrochloride interacts with nucleic acids through intercalation — the planar acridine ring system inserts between stacked base pairs of the DNA or RNA double helix. This mechanism underlies both its fluorescent staining properties and its biological activity in experimental systems, making it a valuable tool for molecular interaction studies, efflux-pump activity assays, and screening assays where a nucleic-acid-binding fluorophore is required.
Typical Working Concentrations
Trace
µg/mL range
Fluorescent DNA/RNA staining, microscopy
Low
µM–mM range
Efflux pump activity / accumulation assays
Assay-dependent
Titrated
Intercalation / binding studies
Historic
Dilute aqueous
Legacy antiseptic use (non-clinical reference only)
💡 Working concentrations are highly application- and instrument-dependent. Titrate against your own assay system before adopting a fixed concentration — this table is a starting-point reference only, not a validated protocol.
Applications
Fluorescent Staining
DNA/RNA visualisation via intercalative binding
Nucleic Acid Interaction Studies
Binding, intercalation and mechanistic assays
Microbial Growth Research
Growth and inhibition studies in culture systems
Oncology & Antiviral Research
Hypoxia-pathway and antiviral screening models
Aquaculture Research
Experimental antimicrobial effect studies
Efflux Pump Assays
Fluorometric substrate for multidrug efflux activity
Cross-Reference / Equivalent Products
| Brand | Product Name | Catalogue No. | Notes |
|---|---|---|---|
| Sigma-Aldrich (Merck) | Acriflavine hydrochloride | A8251 | Imported; longer lead time |
| TCI Chemicals | Acriflavine Hydrochloride | A0677 | Imported; cold/dark storage recommended |
| Loba Chemie | Acriflavin for Biochemistry | 00600 | Imported |
| Spectrum Chemical | Acriflavine Hydrochloride | AC175 | Imported |
| AuSaMicS | Acriflavine Hydrochloride Powder | ASA-2037 | Melbourne stock · same-week dispatch · COA included |
Storage & Shelf Life
Store in a cool, dry place, protected from light and moisture. Keep container tightly sealed. Recommended shelf life: 24 months from date of manufacture under recommended storage conditions. Handle with appropriate PPE — avoid contact with skin, fabrics, and surfaces due to strong staining properties.
Intended Use & Regulatory
For laboratory and research use only. Not for human or animal consumption. Not intended for therapeutic, clinical, diagnostic, or food applications. HS/AHECC Code: 2933.99 — heterocyclic compounds with nitrogen hetero-atom(s), not elsewhere specified.
Intent-Based Search Queries
acriflavine hydrochloride australia acridine dye supplier Melbourne fluorescent DNA stain buy Sigma A8251 alternative Australia TCI A0677 equivalent acriflavine CAS 8063-24-9 efflux pump assay dye Australia nucleic acid intercalating dye local
Related Products
Product Identification
| Product Name | Acriflavine Hydrochloride Powder |
| Catalogue Number | ASA-2037 |
| CAS Number | 8063-24-9 |
| HS / AHECC Code | 2933.99 |
| Chemical Class | Acridine dye derivative |
| Synonyms | Acriflavinium chloride; 3,6-Diamino-10-methylacridinium chloride hydrochloride; Euflavine |
| Manufacturer | AuSaMicS Pty Ltd, Melbourne, Australia |
Physicochemical Specifications
| Parameter | Specification | Method |
|---|---|---|
| Purity (Assay) | ≥ 90.0% (as Acriflavine Hydrochloride) | HPLC |
| Appearance | Orange to dark-red/brown crystalline powder | Visual |
| Loss on Drying | ≤ 6.5% | Gravimetric, 105°C |
| Sulfated Ash | ≤ 1.0% | Gravimetric (residue on ignition) |
| Solubility (water) | Soluble (moderate, ~1–10% w/v) | Visual clarity test |
| Solubility (ethanol) | Soluble | Visual clarity test |
| Solubility (chloroform, ether) | Insoluble | Visual clarity test |
| Melting Point | ~260°C (decomposes) | Capillary melting point |
| Identification | Conforms to reference spectrum / structure | NMR / UV-Vis |
Mode of Action
Acriflavine is a planar tricyclic acridine cation that intercalates between the stacked base pairs of double-stranded DNA (and, to a lesser extent, RNA), inserting perpendicular to the helical axis and partially unwinding the duplex. This intercalative binding is the basis of its use as a fluorescent nucleic acid stain and its historic antimicrobial activity, which arises from interference with DNA replication and transcription in susceptible microorganisms.
General Handling Guidance
1
Prepare stock solutions in distilled or deionised water (moderately soluble) or ethanol for less polar systems. Protect stock solutions from light.
2
Use appropriate PPE — nitrile gloves, safety glasses, and lab coat — as acriflavine will stain skin, fabric and surfaces on contact.
3
Titrate working concentration empirically for your specific staining or assay system; optimal loading varies with sample type, detection method, and instrument sensitivity.
4
Store stock and working solutions at 4°C, protected from light, and discard per your institution's chemical waste procedures for mutagenic/intercalating dyes.
💡 Pro tip: Like other DNA-intercalating dyes (e.g. ethidium bromide, acridine orange), acriflavine solutions and stained material should be treated as potentially mutagenic waste and disposed of accordingly — never down the drain.
Literature References
| # | Reference |
|---|---|
| 1 | Peacocke AR, Skerrett JNH. The interaction of aminoacridines with nucleic acids. Trans Faraday Soc. 1956;52:261–279. [Foundational acridine–nucleic acid intercalation study] |
| 2 | Lerman LS. Structural considerations in the interaction of DNA and acridines. J Mol Biol. 1961;3(1):18–30. [Intercalation model] |
| 3 | Lee YS, et al. Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization. Proc Natl Acad Sci USA. 2009;106(42):17910–17915. [Oncology/hypoxia research application] |
| 4 | Wainwright M. Acridine — a neglected antibacterial chromophore. J Antimicrob Chemother. 2001;47(1):1–13. [Historic antimicrobial use and mechanism] |
📄 Full 16-section GHS SDS available (Australian WHS Regulations 2023 / GHS 7th Edition). Request via support@ausamics.com.au or download below.
Section 1 — Identification
| Product Name | Acriflavine Hydrochloride Powder |
| Catalogue No. | ASA-2037 |
| CAS Number | 8063-24-9 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Phone / Email | +61 412 520 598 | support@ausamics.com.au |
| Emergency | Poisons Information Centre: 13 11 26 (Australia, 24 hr) |
| Intended Use | Fluorescent nucleic acid stain / research biochemical. For laboratory and research use only. |
Section 2 — Hazard Identification
| GHS Classification | Acute Toxicity (Oral), Category 4; Skin Irritation, Category 2; Serious Eye Damage, Category 1; Hazardous to the Aquatic Environment (Chronic), Category 2 |
| Signal Word | DANGER |
| Hazard Pictograms | GHS05 (Corrosion — eye damage) · GHS07 (Exclamation mark — harmful/irritant) · GHS09 (Environment — aquatic toxicity) |
| Hazard Statements | H302 — Harmful if swallowed H315 — Causes skin irritation H318 — Causes serious eye damage H411 — Toxic to aquatic life with long-lasting effects |
| Precautionary Statements | P264, P270, P273, P280, P301+P312+P330, P305+P351+P338+P310, P332+P313, P391, P501 |
Composition
| Component | CAS | Content | GHS Classification |
|---|---|---|---|
| Acriflavine hydrochloride | 8063-24-9 | 90–100% | Acute Tox. 4; Skin Irrit. 2; Eye Dam. 1; Aquatic Chronic 2 (H302, H315, H318, H411) |
First Aid, PPE, Handling & Disposal
| Route / Scenario | Guidance |
|---|---|
| Inhalation | Move to fresh air. If breathing is difficult, seek medical attention. |
| Skin contact | Remove contaminated clothing. Wash skin thoroughly with plenty of water. Seek medical advice if irritation persists. |
| Eye contact | Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do; continue rinsing. Immediately call a Poison Centre or doctor — this substance causes serious eye damage. |
| Ingestion | Rinse mouth. Call a Poison Centre or doctor if the person feels unwell. Do not induce vomiting unless directed by medical personnel. |
| Gloves | Nitrile gloves — mandatory. Acriflavine stains and penetrates skin readily. |
| Eye protection | Chemical safety goggles (not just spectacles) — required due to serious eye damage hazard. |
| Respiratory | P1/P2 dust mask when weighing or handling bulk powder in an unventilated space. |
| Environmental precautions | Avoid release to the environment. Prevent entry into drains, waterways and sewers — toxic to aquatic life with long-lasting effects. |
| Disposal | Dispose of contents/container as hazardous chemical waste per local EPA regulations. Treat spent staining solutions as potentially mutagenic waste, consistent with other DNA-intercalating dyes. |
| Transport | Not classified as Dangerous Goods for standard laboratory quantities under ADG/IATA/IMDG at typical pack sizes; confirm classification for bulk shipments. |
⚠️ Important: Acriflavine Hydrochloride is a GHS "Danger" classified substance — it causes serious eye damage (H318) and skin irritation (H315), and is harmful if swallowed (H302). Full chemical safety goggles, nitrile gloves, and a lab coat are required whenever handling the powder or its solutions. This summary does not replace the full SDS.
Regulatory Information
| Australian Standard | Australian WHS Regulations 2023 / GHS 7th Edition |
| AICIS Status | Compliant |
| HS / AHECC Code | 2933.99 — Heterocyclic compounds with nitrogen hetero-atom(s), n.e.s. |
| SDS Reference | SDS-ASA-2037-ACR | Issue Date: January 2026 | Review: January 2028 |
| Prepared by | Hassan Salimi, Founder & Technical Director, AuSaMicS Pty Ltd |
Certificate of Analysis — ASA-2037
Each batch of Acriflavine Hydrochloride Powder (ASA-2037) is produced under quality-controlled conditions and released with a batch-specific Certificate of Analysis confirming compliance with all physicochemical specifications.
Analytical Results — Typical Specifications
| Test Parameter | Specification | Method | Result |
|---|---|---|---|
| Purity (Assay) | ≥ 90.0% (as Acriflavine Hydrochloride) | HPLC | Conforms |
| Appearance | Orange to dark-red/brown crystalline powder | Visual | Conforms |
| Loss on Drying | ≤ 6.5% | Gravimetric, 105°C | Conforms |
| Sulfated Ash | ≤ 1.0% | Gravimetric (residue on ignition) | Conforms |
| Solubility (water, ethanol) | Soluble | Visual clarity test | Conforms |
| Identification | Conforms to reference spectrum | NMR / UV-Vis | Conforms |
Batch Traceability
📋 Batch Details (printed on label and supplied COA)
Product
Acriflavine Hydrochloride Powder
Catalogue No.
ASA-2037
CAS Number
8063-24-9
Grade
Research Grade
Batch Number
Stated on label
Manufacturing Date
Stated on label
Expiry Date
Stated on label (24 months)
Storage
Cool, dry, dark, sealed
Manufacturer
AuSaMicS Pty Ltd — Melbourne, Australia
COA
Included with every order
Frequently Asked Questions
❓ What makes acriflavine useful as a fluorescent stain?
Its planar acridine ring system intercalates between DNA/RNA base pairs, producing a strong, stable fluorescent signal that is widely used for nucleic acid visualisation and binding studies.
❓ Is ASA-2037 equivalent to Sigma A8251 or TCI A0677?
Yes — ASA-2037 meets the same research-grade specification: ≥90% purity by HPLC, orange to dark-red crystalline appearance, soluble in water and ethanol. Manufactured and packed in Melbourne with same-week dispatch and full COA with every batch.
❓ Do I need to treat spent acriflavine solutions as hazardous waste?
Yes. As a DNA-intercalating dye, spent acriflavine solutions and stained material should be treated as potentially mutagenic waste and disposed of per your institution's chemical waste procedures — consistent with other intercalating dyes such as ethidium bromide.
❓ What PPE is required when handling this product?
Nitrile gloves and full chemical safety goggles are required — acriflavine causes serious eye damage (H318) and skin irritation (H315), and stains skin and fabric readily.
Packaging Options
| Standard Pack Sizes | 5 g · 25 g · 100 g · 500 g |
| Bulk / Custom | Larger quantities available — contact support@ausamics.com.au |
| Packaging | Amber glass or HDPE container; hermetically sealed; full label with batch no., expiry, storage conditions, and GHS hazard labelling |
Manufactured by AuSaMicS Pty Ltd
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ ≥90% purity (HPLC) — research-grade acridine dye
✓ Strong nucleic acid intercalation — reliable fluorescent signal
✓ Full GHS-compliant SDS with every order
✓ Batch COA, TDS, and SDS with every order
✓ Australian stock — same-week dispatch, no import wait
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ ≥90% purity (HPLC) — research-grade acridine dye
✓ Strong nucleic acid intercalation — reliable fluorescent signal
✓ Full GHS-compliant SDS with every order
✓ Batch COA, TDS, and SDS with every order
✓ Australian stock — same-week dispatch, no import wait
⚠️ For laboratory and research use only. Not for human or animal consumption. Not intended for therapeutic, clinical, diagnostic, or food applications. GHS Danger classification — see SDS tab before handling. AuSaMicS Pty Ltd accepts no liability for use outside the intended application.