Campylobacter Enrichment Broth Base (Preston) | Selective Medium | AS‑1167
$45.00 AUD
AuSaMicS Life Science • Selective Enrichment Media
Campylobacter Enrichment Broth Base
Preston Formulation
The internationally recognised selective enrichment base for thermophilic Campylobacter spp. — the leading cause of bacterial foodborne gastroenteritis in Australia. Preston formulation delivers outstanding recovery of C. jejuni, C. coli, and C. lari from food, water, and environmental samples under microaerophilic conditions.
Cat #: AS-1167
ISO 10272-1:2017 Compliant
✓ Australian Stock
🌡️ Microaerophilic 42°C
🚀 Same-Day Dispatch: Australian Stock
🍗 Food Safety: Poultry, meat, dairy, produce
🌡️ 42°C / Microaerophilic: Thermophile-selective
🏆 Campylobacter Detection Specialist
🌡️ 42°C Selectivity — Thermophilic Campylobacter only
🧫 Preston Formulation — ISO 10272 reference method
📋 Complete Package — COA + SDS included
⚡ Fast Supply — Ships within 24h
📞 Expert Support — Protocol optimization
⚠️ Supplement Required
Use with Preston Selective Supplement (polymyxin B, rifampicin, trimethoprim, actidione)
🔬 Technical Overview & Biochemistry
Campylobacter spp. — particularly C. jejuni and C. coli — are the most frequently reported cause of bacterial foodborne illness in Australia, with the majority of cases linked to poultry, undercooked meat, unpasteurised dairy, and contaminated water. Campylobacter Enrichment Broth Base (Preston) is a nutrient-rich selective enrichment medium designed to maximise recovery of these fastidious, microaerophilic organisms from complex, heavily contaminated sample matrices.
Selectivity Strategy (Preston Supplement):
Polymyxin B — Disrupts gram-negative cell membranes; suppresses Pseudomonas, Proteus, and other competing gram-negatives
Rifampicin — Inhibits RNA polymerase in most bacteria; Campylobacter is intrinsically resistant
Trimethoprim — Blocks folate synthesis; suppresses non-Campylobacter flora including Enterobacteriaceae
Actidione (Cycloheximide) — Antifungal; suppresses yeasts and moulds common in food/environmental samples
Horse Blood (5%) — Provides growth factors (haemin, NAD precursors) critical for Campylobacter recovery
Polymyxin B — Disrupts gram-negative cell membranes; suppresses Pseudomonas, Proteus, and other competing gram-negatives
Rifampicin — Inhibits RNA polymerase in most bacteria; Campylobacter is intrinsically resistant
Trimethoprim — Blocks folate synthesis; suppresses non-Campylobacter flora including Enterobacteriaceae
Actidione (Cycloheximide) — Antifungal; suppresses yeasts and moulds common in food/environmental samples
Horse Blood (5%) — Provides growth factors (haemin, NAD precursors) critical for Campylobacter recovery
Result Interpretation: Growth in Preston Broth = enrichment positive. Subculture to Campylobacter selective agar (e.g., CCDA, mCCDA, or Skirrow Agar) for colony confirmation and identification.
⚙️ Mode of Action
Campylobacter spp. are thermophilic microaerophiles requiring reduced oxygen (5% O₂, 10% CO₂, 85% N₂) and elevated temperature (42°C) for optimal growth — conditions that are inherently restrictive to most competing flora. The Preston supplement exploits the natural antibiotic resistance profile of thermophilic Campylobacter: resistance to polymyxin B (due to membrane lipid A modification), rifampicin, and trimethoprim allows selective survival while a broad range of competing gram-positive and gram-negative organisms are suppressed. Horse blood lysate supplies essential haemin and amino acids, supporting the nutritionally demanding growth of Campylobacter during the 24–48 h enrichment phase prior to plating.
📊 Campylobacter Enrichment & Isolation Media Comparison
🧬 Applications
🍗 Food Safety Testing
Detection of thermophilic Campylobacter in poultry, raw meat, dairy, fresh produce, and ready-to-eat foods per ISO 10272-1:2017 and AS 5013.6. Essential for FSANZ compliance and retail audits.
💧 Water Microbiology
Enrichment of Campylobacter from drinking water, surface water, recreational water, and irrigation water — particularly after rainfall events and in catchment risk assessments.
🏥 Clinical Microbiology
Enrichment from faecal specimens, rectal swabs, and biopsy material prior to plating on selective isolation agar for culture-confirmed campylobacteriosis diagnosis.
🌾 Environmental & Veterinary
Abattoir monitoring, poultry farm surveillance, livestock faecal sampling, and environmental surface swabs in food production facilities and veterinary diagnostic applications.
Additional Laboratory Applications:
- Retail Poultry Monitoring: FSANZ food safety programme compliance
- Outbreak Investigation: Epidemiological tracing in foodborne illness clusters
- Research: Antibiotic resistance surveillance, virulence studies
- Dairy Monitoring: Raw milk and cheese safety testing
- NATA Accredited Labs: Accredited food and water microbiology panels
- Export Certification: Meat export testing to EU/US requirements
📋 ISO 10272-1:2017 Procedure — Enrichment Step
1️⃣
Prepare Base
Dissolve 23.0 g/L in purified water. Autoclave 121°C / 15 min. Cool to 47–50°C.
2️⃣
Add Supplement & Blood
Aseptically add 1 vial Preston Supplement + 50 mL sterile defibrinated horse blood per litre. Mix gently.
3️⃣
Homogenise Sample
Add 10 g food sample to 90 mL Preston Broth (1:10). Stomach 30–60 s. Adjust pH to 7.4 if required.
4️⃣
Microaerophilic Incubation
Incubate at 41.5 ± 0.5°C for 24–48 h under microaerophilic atmosphere (5% O₂, 10% CO₂, 85% N₂).
5️⃣
Subculture to Agar
Streak 10 µL enrichment broth onto CCDA or mCCDA Agar. Incubate 41.5°C / 24–48 h microaerophilic.
6️⃣
Confirm Colonies
Confirm grey, flat, spreading colonies by Gram stain, oxidase, and PCR or biochemical ID.
🫙 Microaerophilic Atmosphere Options
Gas Mix Cylinder: 5% O₂ / 10% CO₂ / 85% N₂ — most reliable for enrichment broths
CampyGen Sachets: Convenient sealed jar method for agar plates — not recommended for large-volume broths
Tri-gas Incubator: Ideal for high-throughput labs; maintains consistent atmosphere throughout incubation
⚠️ Critical: Temperature precision is essential — use 41.5 ± 0.5°C (not 37°C) for thermophile selectivity. Microaerophilic conditions must be established within 1 h of inoculation. Aerobic incubation will suppress or kill Campylobacter.
💡 Protocol Optimization Guidelines
Preparation:
Dissolve 23.0 g/L. Autoclave 121°C / 15 min. Cool to 47–50°C before adding supplement and blood aseptically.
Dissolve 23.0 g/L. Autoclave 121°C / 15 min. Cool to 47–50°C before adding supplement and blood aseptically.
pH (25°C):
7.4 ± 0.2 after sterilisation and supplementation
7.4 ± 0.2 after sterilisation and supplementation
Blood Source:
Defibrinated horse blood preferred (5% v/v). Sheep blood is an acceptable alternative but may reduce recovery slightly.
Defibrinated horse blood preferred (5% v/v). Sheep blood is an acceptable alternative but may reduce recovery slightly.
Storage (prepared):
2–8°C, use within 5 days after supplementation. Dehydrated powder: below 25°C, dry and dark. Supplement: store at −20°C.
2–8°C, use within 5 days after supplementation. Dehydrated powder: below 25°C, dry and dark. Supplement: store at −20°C.
🇦🇺 Campylobacteriosis in Australia — Why It Matters
Campylobacter is consistently the most notified foodborne pathogen in Australia, with OzFoodNet reporting over 200 cases per 100,000 population annually. The primary reservoir is poultry — up to 80% of retail chicken carcasses test positive. Effective enrichment is the critical first step in the detection workflow, and the Preston formulation is the ISO reference standard.
📊 #1 notified foodborne pathogen in Australia
🍗 80%+ retail poultry contamination rate
💊 Leading cause of bacterial gastroenteritis hospitalisation
📋 Technical Specifications
| Catalogue Number | AS-1167 |
| Common Name | Preston Enrichment Broth Base |
| Format | Dehydrated powder (base only) |
| Reconstitution | 23.0 g/L in purified water |
| pH (25°C) | 7.4 ± 0.2 |
| Incubation Temp | 41.5 ± 0.5°C |
| Incubation Time | 24–48 hours |
| Atmosphere | Microaerophilic (5% O₂ / 10% CO₂ / 85% N₂) |
| Supplement Required | Yes — Preston Selective Supplement |
| Blood Required | 5% defibrinated horse blood |
| Sterilisation | Autoclave 121°C / 15 min (base only) |
| HS Code | 3821.00.00 |
✅ Quality Control Performance
| Test Organism | ATCC / NCTC | Expected Result | CFU Spec |
|---|---|---|---|
| Campylobacter jejuni | NCTC 11168 | Good growth / enrichment | ≤100 CFU |
| Campylobacter coli | ATCC 33559 | Good growth / enrichment | ≤100 CFU |
| Escherichia coli | ATCC 25922 | Inhibited / no growth | ≤100 CFU |
| Pseudomonas aeruginosa | ATCC 27853 | Inhibited / no growth | ≤100 CFU |
🧪 Typical Base Formulation (per litre)
| Nutrient Broth No. 2 | 25.0 g |
| Lab-Lemco Powder (Meat Extract) | 10.0 g |
| Bacteriological Peptone | 10.0 g |
| Sodium Chloride | 5.0 g |
| + Preston Selective Supplement | 1 vial / L |
| + Defibrinated Horse Blood | 50 mL / L (5%) |
Preston Supplement contains: Polymyxin B (5,000 IU), Rifampicin (10 mg), Trimethoprim (5 mg), Actidione/Cycloheximide (50 mg) per litre of completed medium. Sold separately or available as a kit.
* Formulation may vary slightly between lot numbers. Refer to the current COA for exact composition.
📜 Standards & Regulatory Compliance
- ✓ ISO 10272-1:2017 — Horizontal method for detection and enumeration of Campylobacter
- ✓ AS 5013.6:2009 — Australian Standard for Campylobacter in food
- ✓ FSANZ Food Standards — Australian/NZ food safety compliance
- ✓ FDA BAM Chapter 7 — Campylobacter in foods (US reference)
- ✓ NATA Accreditation — Suitable for NATA-accredited food and water labs
- ✓ OzFoodNet / CDNA — Supports national Campylobacter surveillance reporting
📝 Alternative Names
Campylobacter Enrichment Broth (Preston)
Preston Broth Base
CEB Preston Base
Campylobacter Preston Medium
Preston Selective Enrichment Broth
Thermophilic Campylobacter Enrichment Broth
🔄 Cross-Reference / Equivalent Products
Cross-references are provided for convenience. Catalogue numbers are trademarks of their respective owners. AuSaMicS products are manufactured and quality-tested independently.
🧬 Complete Campylobacter Detection System
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Need Campylobacter Testing Method Support?
Our food safety microbiologists can assist with ISO 10272-1 and AS 5013.6 workflow setup, microaerophilic incubation system selection, NATA compliance documentation, and method validation support.
For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use. Contains actidione (cycloheximide) in the completed supplement — handle with appropriate precautions; refer to SDS before use. Results should be interpreted by qualified laboratory personnel in accordance with applicable standard methods.
AuSaMicS Pty Ltd | ABN: 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074, Australia
www.ausamics.com.au | support@ausamics.com.au | +61 412 520 598
Same-day dispatch • Australian stock • Full documentation included