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Mueller Hinton Broth AS‑1439 | Microbiology Culture Medium | Ausamics Life Science
$35.00 AUD
AuSaMicS Life Science / Dehydrated Culture Media
Mueller Hinton Broth
Low-Thymidine Broth for Antimicrobial Susceptibility Testing
Australian Manufactured CLSI M07 EUCAST ISO 20776-1 ISO 11133:2014 Full GHS Documentation
21 g/LReconstitution
7.3 ± 0.2pH at 25 °C
121 °C / 15 minSterilisation
500 gPack Size
3 YearsShelf Life
Manufactured in Melbourne, Australia
Same-Week Dispatch
Lot-Release QC Tested
TDS / SDS Included with Every Order
Direct Founder Technical Support
Formulation & Composition
| Ingredient | Role | g / Litre |
|---|---|---|
| Beef Extract | Nitrogen source, vitamins, growth factors | 2.0 |
| Casein Hydrolysate (Acid) | Amino acids, peptides, nucleotides | 17.5 |
| Starch | Absorbs toxic fatty acids and metabolites | 1.5 |
| Total | — | 21.0 |
Reconstitute 21 g in 1 litre of purified / deionised water. Mix to dissolve completely. Autoclave at 121 °C for 15 minutes. Do not over-autoclave.
Mode of Action & Biochemistry
Mueller Hinton Broth provides a well-characterised, reproducible nutritional matrix optimised exclusively for antimicrobial susceptibility testing. Its three-component formulation is purpose-designed rather than general-purpose:
1
Casein hydrolysate supplies the free amino acids and short peptides required for rapid aerobic growth while maintaining the chemically-defined consistency needed for reproducible MIC endpoints.
2
Beef extract contributes vitamins, nucleotides, and cofactors that support growth of fastidious organisms without adding excess thymidine — the key interfering molecule in antifolate MIC testing.
3
Soluble starch adsorbs inhibitory fatty acids and other toxic metabolites released from agar or glassware surfaces that would otherwise falsely elevate MIC values for many antibiotic classes.
Low-Thymidine Criticality: Excess thymidine bypasses the dihydrofolate reductase target of trimethoprim and the dihydropteroate synthase target of sulfonamides, producing falsely elevated MIC values and incorrect susceptibility categorisation. Mueller Hinton Broth is formulated and verified to contain thymidine and thymine at levels below CLSI M07 and EUCAST threshold requirements.
Divalent cation concentrations are controlled within CLSI-specified ranges: Ca²⁺ ~50 mg/L and Mg²⁺ ~25 mg/L. These ranges are critical because elevated Mg²⁺ antagonises aminoglycosides and chelation by low Ca²⁺ potentiates tetracyclines — both producing MIC shifts of 2–4 dilution steps if uncontrolled.
Physical & Chemical Characteristics
| Appearance (powder) | Homogeneous cream to light beige free-flowing powder |
| Appearance (prepared) | Clear to slightly opalescent pale yellow broth |
| pH (prepared, 25 °C) | 7.3 ± 0.2 |
| Osmolality (prepared) | ~310 mOsm/kg |
| Thymidine content | Low — verified per CLSI M07 requirement |
| Ca²⁺ concentration | ~50 mg/L (CLSI cation range) |
| Mg²⁺ concentration | ~25 mg/L (CLSI cation range) |
| Water activity (powder) | < 0.50 |
| Loss on drying | < 5% w/w |
Quality Control — Performance Testing
| Test Organism | ATCC Strain | Expected Growth | MIC Reference Range | Antibiotic | Inoculum | Status |
|---|---|---|---|---|---|---|
| Escherichia coli | ATCC 25922 | Good turbidity | 2–8 µg/mL | Ampicillin | ≤ 100 CFU/well | PASS |
| Staphylococcus aureus | ATCC 29213 | Good turbidity | 0.12–0.5 µg/mL | Oxacillin | ≤ 100 CFU/well | PASS |
| Pseudomonas aeruginosa | ATCC 27853 | Good turbidity | 0.5–2 µg/mL | Gentamicin | ≤ 100 CFU/well | PASS |
| Enterococcus faecalis | ATCC 29212 | Good turbidity | 0.5–2 µg/mL | Ampicillin | ≤ 100 CFU/well | PASS |
QC performed per CLSI M07 and ISO 11133:2014. Each production lot tested before release. MIC ranges represent CLSI-published acceptable ranges for these strain/antibiotic combinations.
Preparation Protocol
Step 1 — Weigh: 21 g dehydrated powder per litre of purified water (CLSI Type II or better).
Step 2 — Dissolve: Stir to dissolve completely. Gentle warming permitted; do not boil.
Step 3 — pH Check: Verify pH is 7.3 ± 0.2 at 25 °C before sterilisation. Adjust if necessary with 1 N NaOH or HCl.
Step 4 — Sterilise: Autoclave at 121 °C for 15 minutes. Do not exceed 20 minutes — prolonged heat degrades carbohydrates and alters cation balance.
Step 5 — Cool: Cool to room temperature (or 50 °C for supplements). Dispense aseptically into microtitration plates or tubes.
Step 6 — Store: Prepared broth at 2–8 °C. Use within 7 days. Do not refreeze.
Step 2 — Dissolve: Stir to dissolve completely. Gentle warming permitted; do not boil.
Step 3 — pH Check: Verify pH is 7.3 ± 0.2 at 25 °C before sterilisation. Adjust if necessary with 1 N NaOH or HCl.
Step 4 — Sterilise: Autoclave at 121 °C for 15 minutes. Do not exceed 20 minutes — prolonged heat degrades carbohydrates and alters cation balance.
Step 5 — Cool: Cool to room temperature (or 50 °C for supplements). Dispense aseptically into microtitration plates or tubes.
Step 6 — Store: Prepared broth at 2–8 °C. Use within 7 days. Do not refreeze.
Applications
Primary Applications
- Broth microdilution MIC testing (CLSI M07, EUCAST)
- Automated AST (VITEK 2, BD Phoenix, MicroScan)
- Time-kill kinetics studies
- Antibiotic screening assays
- Synergy testing (checkerboard)
Sectors
- Hospital clinical microbiology labs
- Pharmaceutical QC and R&D
- NATA-accredited reference laboratories
- University microbiology departments
- CROs and biotech companies
- Veterinary diagnostic laboratories
Cross-Reference — Equivalent Products
| Supplier | Product Name | Cat. No. | AuSaMicS Advantage |
|---|---|---|---|
| Oxoid / Thermo Fisher | Mueller Hinton Broth | CM0405 | Australian manufactured — no import lead times |
| BD Difco | Mueller Hinton Broth | 275730 | Full GHS documentation included; same-week dispatch |
| Merck / Sigma-Aldrich | Mueller Hinton Broth | 70192 | Direct founder microbiologist support; competitive pricing |
| HiMedia | Mueller Hinton Broth | M391 | Melbourne stock; Australian regulatory compliance |
Literature & References
- CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. CLSI Standard M07, 11th ed. Wayne, PA: CLSI; 2018.
- EUCAST. Broth microdilution methodology for MIC determination. European Committee on Antimicrobial Susceptibility Testing, 2021.
- ISO 20776-1:2019. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices.
- ISO 11133:2014. Microbiology of food, animal feed and water — preparation, production, storage and performance testing of culture media.
- Mueller JH, Hinton J. (1941). A protein-free medium for primary isolation of the Gonococcus and Meningococcus. Proc Soc Exp Biol Med. 48(1):330–333.
- Barry AL, et al. (1992). Cation adjustments of Mueller Hinton broth. Antimicrob Agents Chemother. 36(5):957–960.
This Technical Data Sheet is issued by AuSaMicS Pty Ltd (ABN: 56 676 640 467), 31 Longview CT, Thomastown VIC 3074, Australia. www.ausamics.com.au | support@ausamics.com | +61 412 520 598. For laboratory, research, and professional use only. Performance data is representative of typical lots tested under AuSaMicS standard conditions. AuSaMicS Pty Ltd accepts no liability for results obtained outside validated conditions. Specifications are subject to change without notice; verify with current lot COA. Information current as of date of publication.
| Product Name | Mueller Hinton Broth |
| Catalogue Number | AS-1439 |
| Supplier | AuSaMicS Pty Ltd, 31 Longview CT, Thomastown VIC 3074, Australia |
| ABN | 56 676 640 467 |
| Emergency Contact | +61 412 520 598 | support@ausamics.com |
| Poison Information | Australia: 13 11 26 (Poisons Information Centre) |
| SDS Version | Refer to product label / current lot documentation |
| Standard | GHS (7th Rev.) — Australian WHS Regulations |
Section 2 — Hazard Identification
Not classified as hazardous — GHS / Safe Work Australia No GHS hazard pictograms, signal word, or hazard statements are assigned under Safe Work Australia GHS criteria. This product does not meet the threshold criteria for any hazard category in the Hazardous Chemical Information System (HCIS).
| GHS Classification | Not classified as hazardous (Safe Work Australia) |
| Signal Word | None required |
| Hazard Statements | None assigned |
| Pictograms | None required |
| Precautionary Statements | P260 — Do not breathe dust | P264 — Wash hands thoroughly after handling | P272 — Contaminated work clothing not to leave the workplace |
Laboratory Biosafety Notice: Mueller Hinton Broth is a microbiological growth medium. Once inoculated, it supports rapid growth of bacteria including potential pathogens. Handle inoculated material under appropriate biosafety containment determined by the risk group of the organisms under study. Follow your institution's biosafety manual and applicable Australian Standard AS/NZS 2243.3.
Section 3 — Composition / Information on Ingredients
| Component | CAS Number | Proportion (%) | HCIS Classification |
|---|---|---|---|
| Beef Extract | N/A (natural extract) | ~9.5 | Not classified |
| Casein Hydrolysate (Acid) | 65072-00-6 | ~83.3 | Not classified |
| Starch (soluble) | 9005-84-9 | ~7.1 | Not classified |
All components listed on the Australian Inventory of Chemical Substances (AICS). No components at or above classification thresholds.
Section 4 — First Aid Measures
| Eye Contact | Flush immediately with copious water for at least 15 minutes, holding eyelids open. Seek medical attention if irritation persists. |
| Skin Contact | Wash affected area with soap and water. Remove contaminated clothing. Seek medical attention if irritation develops. |
| Inhalation | Remove to fresh air. Seek medical attention if respiratory symptoms develop. |
| Ingestion | Do not induce vomiting. Rinse mouth with water. Seek medical advice. Show SDS to treating physician. |
| Most Important Symptoms | Dust may cause mild respiratory or eye irritation. No systemic hazard expected from uninoculated material. |
Section 5 — Fire-Fighting Measures
| Flammability | Not flammable under normal conditions |
| Suitable Extinguishing Media | Appropriate for surrounding fire (water spray, CO₂, dry chemical, foam) |
| Special Hazards | Thermal decomposition may produce carbon monoxide, carbon dioxide, nitrogen oxides |
| Protective Equipment | Self-contained breathing apparatus and full protective equipment for fire in area |
Section 6 — Accidental Release Measures
| Personal Precautions | Avoid generating dust. Wear lab coat, gloves, and safety glasses. Ensure adequate ventilation. |
| Environmental Precautions | Prevent large quantities entering drains or waterways. |
| Spill Cleanup (dry) | Sweep or vacuum carefully to avoid dust. Place in sealed labelled waste container. |
| Spill Cleanup (prepared) | Absorb with inert material (vermiculite, dry sand). Collect in sealed waste container. If inoculated: inactivate by autoclaving (121 °C, 30 min) before disposal. |
Section 7 — Handling & Storage
| Handling | Use in well-ventilated area. Avoid generating dust. Wear appropriate PPE (lab coat, nitrile gloves, safety glasses). Wash hands after handling. Do not eat, drink, or smoke in handling area. |
| Storage (powder) | 15–30 °C. Dry conditions. Away from direct sunlight. Keep container tightly closed after opening. Do not store near strong oxidising agents. |
| Storage (prepared broth) | 2–8 °C. Use within 7 days. Do not refreeze. Discard if turbid or discoloured before use. |
| Incompatibilities | Strong oxidising agents, strong acids, strong bases |
Section 8 — Exposure Controls & Personal Protection
| Occupational Exposure Limits | No specific WES (Workplace Exposure Standards) established for this mixture (Safe Work Australia) |
| Engineering Controls | Local exhaust ventilation recommended when handling large quantities of powder. General laboratory ventilation sufficient for routine use. |
| Respiratory Protection | Not required for routine laboratory use. P2 dust mask for bulk handling operations generating significant dust. |
| Hand Protection | Nitrile or latex laboratory gloves |
| Eye / Face Protection | Safety glasses or goggles |
| Body Protection | Laboratory coat |
Section 9 — Physical & Chemical Properties
| Physical State | Powder (dehydrated) |
| Colour | Cream to light beige |
| Odour | Faint, characteristic (peptone/meat extract) |
| pH (prepared, 25 °C) | 7.3 ± 0.2 |
| Boiling Point | Not applicable (solid) |
| Melting / Decomposition | > 200 °C (decomposition) |
| Vapour Pressure | Negligible at ambient temperature |
| Water Solubility | Soluble (21 g/L, prepared broth) |
| Flash Point | Not applicable |
| Flammability | Not flammable |
Section 10 — Stability & Reactivity
| Stability | Stable under recommended storage conditions |
| Conditions to Avoid | Heat, moisture, direct sunlight, strong oxidising conditions |
| Hazardous Decomposition Products | Carbon oxides (CO, CO₂), nitrogen oxides — only under combustion conditions |
| Hazardous Reactions | None known under normal laboratory use conditions |
| Hazardous Polymerisation | Will not occur |
Section 11 — Toxicological Information
| Acute Toxicity (oral) | Not classified. Components are food-grade or pharmaceutical-grade materials. |
| Skin Corrosion / Irritation | Not classified |
| Eye Damage / Irritation | Not classified. Dust may cause mild transient irritation. |
| Respiratory Sensitisation | Not classified |
| Carcinogenicity | No components listed by IARC, NTP, or ACGIH as carcinogens |
| Reproductive Toxicity | Not classified |
| STOT — Single Exposure | Not classified |
| Aspiration Hazard | Not classified |
Section 12 — Ecological Information
| Aquatic Toxicity | No data available for the mixture. Components are natural biological materials. Not expected to be acutely toxic to aquatic organisms at typical use concentrations. |
| Persistence / Biodegradability | Expected to be biodegradable (natural proteinaceous material) |
| Bioaccumulation | Not expected to bioaccumulate |
| Mobility in Soil | Soluble in water; expected to be retained in soil |
Section 13 — Disposal Considerations
Biosafety Disposal: Uninoculated prepared broth may be disposed of via drain (dilute). All inoculated or potentially contaminated material must be inactivated by autoclaving (121 °C, minimum 30 minutes) or chemical disinfection before drain disposal. Solid waste: dispose in sealed containers to approved landfill in accordance with state/territory and local regulations.
| Waste Disposal Method | Inactivate by autoclaving before drain disposal. Solid/contaminated material: approved biohazard waste contractor. |
| Regulatory Reference | Environment Protection Act (Vic); Local Council regulations; AS/NZS 3816 (management of clinical and related wastes) |
Section 14 — Transport Information
| ADG (Australian Dangerous Goods) | Not a Dangerous Good |
| IATA (Air) | Not restricted |
| IMDG (Sea) | Not restricted |
| UN Number | Not applicable |
| Packing Group | Not applicable |
| Special Precautions | Protect from moisture during transport. Keep dry and away from heat sources. |
Section 15 — Regulatory Information
| Australian WHS Regulations | Not classified as a Hazardous Chemical under the Safe Work Australia GHS criteria |
| AICS (Australian Inventory) | All components listed on the Australian Inventory of Chemical Substances |
| Poison Schedule | Not scheduled — not a therapeutic good or regulated poison |
| NATA / ISO 11133 | Quality system aligned with ISO 11133:2014 culture media performance testing |
Section 16 — Other Information
| Intended Use | For laboratory, research, pharmaceutical QC, and diagnostic professional use only. Not for human consumption, medicinal, or food use. |
| Manufacturer | AuSaMicS Pty Ltd | ABN 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074 | www.ausamics.com.au |
| Technical Support | support@ausamics.com | +61 412 520 598 |
| Preparation / Revision | Refer to current lot documentation for version date |
| Key References | Safe Work Australia GHS (7th Rev.); GHS Purple Book 7th ed.; AS/NZS 2243.3; ISO 11133:2014 |
This Safety Data Sheet is prepared in accordance with the GHS (7th Revised Edition) and Australian WHS Regulations. It is provided in good faith and represents the best available information at the time of issue. AuSaMicS Pty Ltd (ABN: 56 676 640 467) shall not be liable for any damages arising from the use of this information or from reliance on its completeness. Users must determine suitability for their specific application. This SDS is superseded by any lot-specific version issued with the product.
AuSaMicS Pty Ltd | ABN 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074 support@ausamics.com | +61 412 520 598 | www.ausamics.com.au AS-1439 — Mueller Hinton Broth