Cooked Meat Medium | Anaerobic Bacteria Cultivation & Maintenance | AS‑1183
Cooked Meat Medium
Enriched Reducing Medium for Cultivation and Maintenance of Anaerobic Bacteria
Cooked Meat Medium (CMM) is a highly enriched reducing medium designed for the cultivation, maintenance, and recovery of obligate anaerobes, particularly Clostridium species. The presence of cooked meat particles provides nutrients and creates a low oxidation-reduction potential environment essential for anaerobic growth.
🏆 Anaerobic Standard
🔬 Principle of the Medium
Cooked Meat Medium contains finely minced cooked meat particles which act as both a nutrient source and a reducing agent. These particles help lower the oxidation-reduction potential, creating an environment favorable for obligate anaerobes.
The medium also includes peptones and buffering components to support microbial metabolism, while the meat substrate can absorb toxic metabolites and maintain stable growth conditions.
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Growth of strict anaerobes
🧪 Applications
- Cultivation of Clostridium spp. (e.g. C. perfringens, C. botulinum)
- Recovery of stressed anaerobic bacteria
- Maintenance and storage of anaerobic cultures
- Clinical, food, and environmental microbiology
📊 Anaerobic Media Comparison
| Medium | Type | Best Use | Key Advantage |
|---|---|---|---|
| Cooked Meat Medium | Reducing broth | Anaerobic recovery & maintenance | Strong redox reduction |
| Thioglycollate Broth | Reducing broth | Aerotolerance testing | Gradient oxygen system |
| Robertson’s Cooked Meat | Enriched broth | Clostridium culture | Classical anaerobic standard |
| Fluid Thioglycollate Medium | Reducing medium | Sterility testing | Pharma compliance |
💡 Practical Notes
- Meat particles enhance anaerobic conditions
- Supports toxin production studies
- Useful for long-term culture maintenance
- Observe blackening or digestion as metabolic indicators
📋 Technical Specifications
| Product Code | AS-1183 |
| Format | Dehydrated medium / meat particles |
| Type | Reducing enriched medium |
| Key Component | Cooked meat particles |
| Final pH | ~7.0 ± 0.2 |
| Storage | 15–25°C dry |
| HS Code | 3821.00.00 |
🧫 Growth Indicators
- ✓ Turbidity → microbial growth
- ✓ Meat digestion → proteolysis
- ✓ Blackening → sulfide production
✅ Quality
- ✓ Strong anaerobic performance
- ✓ Reliable recovery of Clostridium
- ✓ COA available
Disclaimer: For laboratory and research use only.