Cooked Meat Medium | Anaerobic Bacteria Cultivation & Maintenance | AS‑1183

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AuSaMicS Life Science • Anaerobic Media

Cooked Meat Medium

Enriched Reducing Medium for Cultivation and Maintenance of Anaerobic Bacteria

Cooked Meat Medium (CMM) is a highly enriched reducing medium designed for the cultivation, maintenance, and recovery of obligate anaerobes, particularly Clostridium species. The presence of cooked meat particles provides nutrients and creates a low oxidation-reduction potential environment essential for anaerobic growth.

AS-1183 Anaerobic Medium Clostridium spp. ✓ Reducing System
🧫 Strict anaerobe recovery ⚗️ Low redox environment 🔬 Supports toxin-producing organisms

🏆 Anaerobic Standard

🦠 Clostridium recovery
⚗️ Strong reducing conditions
📋 COA + SDS available
🔬 Long-term culture maintenance
Key Function
Supports strict anaerobic growth

🔬 Principle of the Medium

Cooked Meat Medium contains finely minced cooked meat particles which act as both a nutrient source and a reducing agent. These particles help lower the oxidation-reduction potential, creating an environment favorable for obligate anaerobes.

The medium also includes peptones and buffering components to support microbial metabolism, while the meat substrate can absorb toxic metabolites and maintain stable growth conditions.

Cooked meat particles → Reduced environment (low Eh)

Growth of strict anaerobes

🧪 Applications

  • Cultivation of Clostridium spp. (e.g. C. perfringens, C. botulinum)
  • Recovery of stressed anaerobic bacteria
  • Maintenance and storage of anaerobic cultures
  • Clinical, food, and environmental microbiology

📊 Anaerobic Media Comparison

Medium Type Best Use Key Advantage
Cooked Meat Medium Reducing broth Anaerobic recovery & maintenance Strong redox reduction
Thioglycollate Broth Reducing broth Aerotolerance testing Gradient oxygen system
Robertson’s Cooked Meat Enriched broth Clostridium culture Classical anaerobic standard
Fluid Thioglycollate Medium Reducing medium Sterility testing Pharma compliance

💡 Practical Notes

  • Meat particles enhance anaerobic conditions
  • Supports toxin production studies
  • Useful for long-term culture maintenance
  • Observe blackening or digestion as metabolic indicators

📋 Technical Specifications

Product Code AS-1183
Format Dehydrated medium / meat particles
Type Reducing enriched medium
Key Component Cooked meat particles
Final pH ~7.0 ± 0.2
Storage 15–25°C dry
HS Code 3821.00.00

🧫 Growth Indicators

  • ✓ Turbidity → microbial growth
  • ✓ Meat digestion → proteolysis
  • ✓ Blackening → sulfide production

✅ Quality

  • ✓ Strong anaerobic performance
  • ✓ Reliable recovery of Clostridium
  • ✓ COA available

Disclaimer: For laboratory and research use only.

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