Enteric Fermentation Base | Carbohydrate Fermentation for Enteric Bacteria | AS‑1220

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AuSaMicS Life Science • Culture Media

Enteric Fermentation Base

Basal Medium for Carbohydrate Fermentation Testing of Enteric Bacteria

Enteric Fermentation Base is a carbohydrate-free basal medium used with added sugars and an indicator to differentiate microorganisms on the basis of fermentation reactions. Designed to support growth while remaining free of fermentable carbohydrates, it is ideal for building customized fermentation systems for enteric organisms and related bacterial identification workflows.

AS-1220 Fermentation Base ✓ Custom Sugar Addition Enteric Differentiation
🧪 Carbohydrate-free base 🔬 Supports growth of test organisms ⚙️ Flexible formulation platform

🏆 Flexible Fermentation Platform

🦠 Supports enteric test organisms
⚗️ Add sugar of choice + indicator
📋 COA + SDS available
🧫 Suitable for identification workflows
🔬 Useful in teaching and QC labs
Key Advantage
One base, multiple carbohydrate differentiation systems

🔬 Principle of the Medium

A basal medium used for fermentation testing must support the growth of test organisms while remaining free from fermentable carbohydrates. Enteric Fermentation Base is specifically described as a medium used with added carbohydrate and indicator for differentiation of microorganisms based on fermentation reactions. :contentReference[oaicite:0]{index=0}

Standard formulations list beef extract, peptic digest of animal tissue, and sodium chloride as the core ingredients, providing growth support without built-in sugar fermentation bias. :contentReference[oaicite:1]{index=1}

Basal nutrient medium
+ added carbohydrate
+ pH indicator / Durham tube (optional)

Acid and/or gas detection from fermentation

🧬 Applications

🦠 Enteric Identification

Used to differentiate enteric bacteria by supplementing the base with specific carbohydrates such as glucose, lactose, sucrose, mannitol, or other test sugars depending on the identification scheme.

🔬 Custom Fermentation Panels

Ideal for creating customized fermentation media systems in diagnostic, teaching, and research laboratories where multiple carbohydrate reactions are assessed from one standardized base.

🏫 Teaching & Training Labs

Well suited for teaching carbohydrate utilization, acid production, and gas formation using indicator systems and optional Durham tube setups.

✅ QC & Method Development

Useful in quality control and formulation work where a neutral basal medium is needed to validate fermentation reactions with controlled carbohydrate inputs.

📊 Fermentation Media Comparison

Medium Type Primary Role Key Feature
Enteric Fermentation Base Carbohydrate-free basal medium Flexible fermentation differentiation Add sugar and indicator as needed
Purple Broth Base Basal fermentation broth Qualitative fermentation testing Typically uses bromcresol purple and 1% carbohydrate
Andrade Peptone Water Basal carbohydrate medium Fermentation reaction studies Common classical sugar fermentation system
MacConkey Agar Base Selective differential agar base Colony-level carbohydrate differentiation Adds selectivity and solid medium format

💡 Practical Notes

Medium Type:
Basal fermentation broth
Supplementation:
Add chosen carbohydrate and indicator before use
Gas Detection:
Optional Durham tube setup may be used
Workflow Fit:
Differentiation, teaching, QC, method development

📋 Technical Specifications

Product Enteric Fermentation Base
Cat. No. AS-1220
Format Dehydrated powder
Medium Type Basal carbohydrate-free fermentation medium
Core Composition Beef extract 3.0 g/L, peptic digest of animal tissue 10.0 g/L, sodium chloride 5.0 g/L
Final pH 7.2 ± 0.1 at 25°C
Prepared Appearance Clear light amber broth
Storage 10–30°C, dry, tightly closed
HS Code 3821.00.00

🧫 Interpretation Framework

  • ✓ Add the carbohydrate relevant to the identification panel
  • ✓ Indicator change shows acid production
  • ✓ Durham tube may reveal gas formation

✅ Quality

  • ✓ Neutral, reproducible basal formulation
  • ✓ Supports broad fermentation workflows
  • ✓ COA issued per lot

Disclaimer: For laboratory and research use only. Not for household, food, or therapeutic use.

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