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Eugon LT 100 Broth | Neutralizing Diluent & Enrichment Medium for Cosmetics | AS-1228
Eugon LT 100 Broth (AS-1244)
$145.00 AUD
AuSaMicS Life Science • Cosmetic Microbiology Media
Eugon LT 100 Broth
Neutralizing Diluent & Enrichment Medium for Cosmetic Microbiology
Highly practical neutralizing and enrichment broth for recovery of microorganisms in cosmetic products with or without preservatives. Designed for cosmetic microbiology workflows as a preparation diluent and enrichment medium for mesophilic aerobic bacteria and related organisms.
AS-1228
🧫 Neutralizing Diluent
✓ Cosmetic Testing
🧪 Preservative Neutralization
Industry: Cosmetics
Reconstitution: ~37.4 g/L
Use: Diluent + enrichment
Cosmetic Recovery Specialist
Tryptone + Soy Peptone — nutrient-rich support
Lecithin + Polysorbate 80 — preservative neutralization support
Sodium Sulfite — aldehyde neutralization support
Triton / surfactant system — microbial dispersion and release
ISO Cosmetic Workflow Fit
Neutralization + enrichment
Overview
Eugon LT 100 Broth is a liquid medium used as a neutralizing diluent and as an enrichment medium for microorganisms in cosmetic products with and without preservatives. It is positioned for cosmetic microbiology testing and is associated with ISO-based workflows for detection of mesophilic aerobic bacteria and related organisms in cosmetic matrices. citeturn769207search0turn769207search1turn769207search12
Unlike simple nutritive diluents, Eugon LT 100 Broth incorporates ingredients that help neutralize antimicrobial preservatives and disperse microorganisms released from the test sample. This improves recovery when preservatives, surfactants, or cosmetic matrix effects might otherwise suppress viable counts. citeturn769207search6turn769207search11turn769207search13
This makes the medium particularly valuable as both a preparative diluent and an enrichment broth in cosmetic microbiology laboratories working with products that may contain quaternary ammonium compounds, phenolic derivatives, aldehydes, and related preservative systems. citeturn769207search6turn769207search13
Principle of the Medium
Eugon LT 100 Broth combines a rich nutritive base with neutralizing and dispersing components. Tryptone and papainic digest of soybean support microbial growth; glucose provides fermentable energy; sodium chloride supports osmotic balance; and L-cystine improves growth performance. Neutralizing agents such as lecithin, polysorbate 80, and sodium sulfite help counter the action of certain preservatives present in cosmetic products. citeturn769207search11turn769207search6turn769207search13
Triton X-100 or related surfactant systems in some commercial formulas improve dispersion of microorganisms from the cosmetic matrix, helping recover viable organisms more effectively for subsequent enrichment or enumeration. citeturn769207search6turn769207search13
As a result, the medium is useful both for preparing test suspensions and for enrichment of microorganisms from preservative-containing products. citeturn769207search0turn769207search1
Applications
- Preparation of test suspensions for cosmetic microbiology analysis
- Neutralizing diluent for cosmetic products with preservatives
- Enrichment of mesophilic aerobic bacteria from cosmetic products
- Support medium for recovery of yeast and mould present in cosmetics
- Related workflows involving selective enumeration of target organisms such as Pseudomonas in cosmetic testing programs
Complete Formulation & Functional Profile
Typical Composition (per Liter)
| Ingredient | Concentration | Function | Technical Role |
|---|---|---|---|
| Pancreatic digest of casein / tryptone | 15.0 g | Primary nutrient source | Supports broad microbial recovery |
| Papainic digest of soybean | 5.0 g | Secondary nutrient support | Adds peptides and growth-supporting factors |
| Glucose | 5.5 g | Energy source | Supports recovery and growth |
| Sodium chloride | 4.0 g | Osmotic balance | Maintains suitable environmental conditions |
| L-cystine | 0.7 g | Growth enhancement | Improves propagation of microorganisms |
| Lecithin | 1.0 g | Neutralizer | Helps neutralize quaternary ammonium compounds |
| Polysorbate 80 | 5.0 g | Neutralizer / dispersant | Supports neutralization and sample dispersion |
| Sodium sulfite | 0.2 g | Neutralizer | Helps neutralize aldehydes and oxidizing effects |
| Surfactant system (e.g. Triton / related) | 1.0 g | Dispersion support | Improves release and distribution of microorganisms |
Typical reconstitution is about 37.4 g/L, with final pH around 7.0 ± 0.2 depending on manufacturer specification. citeturn769207search6turn769207search11turn769207search1
Neutralization Logic
Preservative Counteraction
Lecithin, polysorbate 80, and sodium sulfite help reduce inhibitory preservative effects.
Lecithin, polysorbate 80, and sodium sulfite help reduce inhibitory preservative effects.
Sample Dispersion
Surfactant components improve release and distribution of microbes from cosmetic matrices.
Surfactant components improve release and distribution of microbes from cosmetic matrices.
Growth Recovery
Rich nutrients support low-level and stressed microbial recovery.
Rich nutrients support low-level and stressed microbial recovery.
Practical Note: This medium is especially useful when the sample matrix or preservative system may otherwise suppress recoverable viable counts.
Cosmetic Microbiology Workflow Comparison
| Medium | Primary Role | Strength | Limitation | Best Use |
|---|---|---|---|---|
| Eugon LT 100 Broth | Neutralizing diluent + enrichment | Good balance of recovery, neutralization, and dispersion | Cosmetic-specific rather than broad food/water use | Cosmetic microbiology workflows |
| Eugon Broth | General nutritive enrichment | Highly nutritious basal medium | Less optimized for preservative neutralization | General nonclinical cultivation |
| Soybean-Casein Digest Broth | General microbial recovery | Common broad-spectrum medium | Neutralization support may be insufficient alone | Routine broad cultivation |
| Selective media for Pseudomonas / fungi | Target-specific downstream testing | Improved selectivity in later stage | Not a primary neutralizing preparation broth | Post-recovery selective workflows |
Selection Guide
Choose Eugon LT 100 if:
• Cosmetic products contain preservatives
• A single medium should act as diluent and enrichment system
• Recovery of low-level microorganisms is important
• Cosmetic products contain preservatives
• A single medium should act as diluent and enrichment system
• Recovery of low-level microorganisms is important
Choose a general broth if:
• Neutralization of preservatives is not a major concern
• The sample matrix is microbiologically simple and non-inhibitory
• Neutralization of preservatives is not a major concern
• The sample matrix is microbiologically simple and non-inhibitory
Choose selective media later if:
• You are moving from recovery/enrichment into target-specific enumeration or confirmation steps
• You are moving from recovery/enrichment into target-specific enumeration or confirmation steps
Quality Positioning & Workflow Value
Typical Use:
Preparation and enrichment in cosmetic microbiology
Preparation and enrichment in cosmetic microbiology
Industry Fit:
Products with or without preservatives
Products with or without preservatives
Commercial Position:
Core specialist medium for cosmetic QC labs
Core specialist medium for cosmetic QC labs
Method Link:
ISO-based cosmetic microbiology workflows
ISO-based cosmetic microbiology workflows
Commercial Positioning: A high-value specialist broth for cosmetic microbiology labs that need reproducible preservative neutralization and reliable enrichment in one workflow-compatible system.
Technical Specifications
| Cat. No. | AS-1228 |
| Medium Type | Neutralizing diluent and enrichment broth |
| Industry | Cosmetics |
| Reconstitution | Approx. 37.4 g/L |
| Final pH | 7.0 ± 0.2 |
| Typical Targets | Mesophilic aerobes, related bacteria, yeast and mould recovery support |
| Sterilization | 121 °C for 15 minutes |
| Use Modes | Test suspension diluent and enrichment medium |
| HS Code | 3821.00.00 |
Preparation Protocol
- 1 Suspend the required quantity of dehydrated medium in purified water.
- 2 Heat gently with mixing until fully dissolved.
- 3 Sterilize at 121 °C for 15 minutes.
- 4 Cool before use as diluent or enrichment medium according to the validated cosmetic microbiology method.
Pack Sizes & Suggested Pricing
| 100 g | AUD 42 |
| 250 g | AUD 88 |
| 500 g | AUD 145 |
| 1 kg | AUD 245 |
| 5 kg | AUD 950 |
ISO & Standards Context
- Associated with ISO cosmetic microbiology workflows including ISO 21149
- Eugon-based media are also discussed in relation to broader cosmetic microbial quality contexts such as ISO 16212, ISO 17516, and ISO 18415 depending on workflow stage. citeturn769207search1turn769207search12
- Suitable for routine QC and preservative-challenge-related microbiological assessment support
Related Products
- Eugon LT 100 Agar
- Eugon Broth
- Selective Pseudomonas media for cosmetic testing
- Yeast and mould detection media for cosmetics
AuSaMicS Compliance & Supply Notes
Supplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.
For laboratory and research use only. Not for household, therapeutic, or pharmaceutical applications.
AuSaMicS Pty Ltd
31 Longview CT, Thomastown, VIC 3074, Australia
ABN 56676640467
www.ausamics.com.au
support@ausamics.com
+61 412 520 598