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Fermentation Medium for Staphylococcus and Micrococcus | Carbohydrate Utilization Test | AS‑1230
$115.00 AUD
AuSaMicS Life Science • Culture Media
Fermentation Medium for Staphylococcus and Micrococcus
Differential Glucose Fermentation Medium for Catalase-Positive Gram-Positive Cocci
Specialized differential medium for distinguishing Staphylococcus from Micrococcus based on anaerobic glucose fermentation. The formulation supports clear acid detection with bromocresol purple while low agar content helps maintain reduced-oxygen conditions within the depth of the tube.
AS-1230
Staphylococcus vs Micrococcus
✓ Differential Test Medium
FDA BAM Style Use
🧪 Glucose Fermentation Test
🔬 Useful in clinical and food microbiology
🟣 Bromocresol Purple Indicator
🏆 Differential ID Support
🦠 Helps separate Staphylococcus from Micrococcus
⚗️ Detects anaerobic acid production from glucose
📋 COA + SDS available
🧫 Tube-based differential workflow
🍽️ Useful in food and clinical labs
Key Readout
Purple to yellow shift = acid formation
🔬 Principle of Differentiation
This medium is used to differentiate Staphylococcus and Micrococcus based on their ability to produce acid from glucose under anaerobic conditions. In standard use, Staphylococcus gives an acid reaction whereas Micrococcus generally does not ferment glucose anaerobically.
Tryptone and yeast extract provide essential nutrients, glucose acts as the fermentable carbohydrate, and bromocresol purple serves as the visual pH indicator. A small amount of agar helps create reduced oxygen conditions deeper in the tube.
Glucose fermentation under anaerobic conditions
↓
Acid production
↓
Bromocresol purple shifts toward yellow
↓
Acid production
↓
Bromocresol purple shifts toward yellow
🧬 Applications
🏥 Clinical Differentiation
Supports differentiation of catalase-positive Gram-positive cocci in routine diagnostic workflows where Staphylococcus and Micrococcus need to be separated.
🍽️ Food Microbiology
Useful as a confirmatory differential test for suspected staphylococcal isolates recovered during food microbiology investigations and BAM-style workflows.
🔬 Character Testing
Suitable for studying anaerobic carbohydrate metabolism as part of organism characterization and identification panels.
🧫 Tube-Based Confirmation
Works well in duplicate tube testing formats where surface overlay is used to reinforce anaerobic conditions during incubation.
📊 Comparative Identification Workflow
💡 Use & Interpretation Notes
Format:
Tube-based differential medium
Tube-based differential medium
Indicator:
Bromocresol purple for acid detection
Bromocresol purple for acid detection
Anaerobic Setup:
Low agar depth; oil overlay may be used in practice
Low agar depth; oil overlay may be used in practice
Interpretation:
Yellowing indicates acid production from glucose
Yellowing indicates acid production from glucose
📋 Technical Specifications
| Product Name | Fermentation Medium for Staphylococcus and Micrococcus |
| Catalog Number | AS-1230 |
| Format | Dehydrated powder |
| Medium Type | Differential glucose fermentation medium |
| Typical Composition Basis | Tryptone, yeast extract, glucose, bromocresol purple, low agar |
| Typical pH | About 7.0 ± 0.2 |
| Prepared Appearance | Purple tube medium |
| Storage | Store dry at 10–30°C in tightly closed container |
| HS Code | 3821.00.00 |
🧫 Interpretation Guide
- ✓ Positive: yellow acid reaction in the anaerobic zone
- ✓ Typical Staphylococcus: ferments glucose anaerobically
- ✓ Typical Micrococcus: no anaerobic glucose fermentation
- ✓ Best interpreted with complementary ID tests
✅ Quality Standards
- ✓ Controlled indicator performance
- ✓ Reproducible differential reaction
- ✓ COA available per lot
- ✓ Suitable for institutional procurement
Related Staphylococcal Identification Media
Disclaimer: For laboratory and research use only. Not for food, household, therapeutic, or direct diagnostic use without appropriate validation.