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Gelatin Peptone Agar | AS-1406 | Standard Plate Count Base | AuSaMicS
$35.00 AUD
AuSaMicS Life Science • Simple Non-Selective Agar for Non-Fastidious Bacteria
Gelatin Peptone Agar
Gelatin Peptone Agar is a simple, non-selective solid medium containing gelatin peptone as the principal nutrient source. It is suitable for cultivation of non-fastidious bacteria and can be used for plate count applications where a simple, low-complexity medium is preferred.
Cat No.: AS-1406
Simple Agar
Non-SelectiveAustralian Stock
Format: Dehydrated culture medium SKU: To be assigned Support: COA / technical support available
Application Summary
Professional laboratory use
Reliable workflow integration
Australian stock
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Overview & Applications
Gelatin Peptone Agar is a simple, non-selective solid medium containing gelatin peptone as the principal nutrient source. It is suitable for cultivation of non-fastidious bacteria and can be used for plate count applications where a simple, low-complexity medium is preferred.
Primary Applications
- Cultivation of non-fastidious bacteria
- Simple plate count work
- General microbiology and teaching labs
- Useful in dairy and related routine enumeration workflows
Key Features
- Very simple two-component formulation
- Non-selective and easy to interpret
- Based on gelatin peptone nutrient profile
- Neutral pH for broad routine cultivation
Typical Composition / Product Characteristics
| Component / Attribute | Amount / Value | Function | Practical Role |
|---|---|---|---|
| Gelatin peptone | 5.0 g/L | Primary nutrient source | Provides peptides and amino acids |
| Agar | 15.0 g/L | Solidifying agent | Creates firm plate surface |
| Final pH | 7.0 ± 0.1 | Neutral growth condition | Supports routine cultivation |
| Medium character | Non-selective | General cultivation | Simple background and straightforward colony observation |
Preparation, Incubation & Interpretation
Preparation
Suspend 20.0 g in 1 L distilled or purified water. Heat to boiling with agitation to dissolve completely. Sterilize by autoclaving at 121°C for 20 minutes. Cool to 45–50°C and pour into sterile Petri dishes.
Incubation / Use
Typical incubation: 35–37°C for about 18–48 hours, depending on the organism and protocol.
Interpretation
Because the medium is non-selective and non-differential, interpretation is based mainly on growth quantity, colony appearance, and any downstream identification tests.
Comparative Media / Workflow Position
| Medium / Product | Primary Target | Principle | Typical Use | Key Advantage |
|---|---|---|---|---|
| Gelatin Peptone Agar | Non-fastidious bacteria | Simple non-selective agar | General cultivation / plate count | Very clean, simple formula |
| Nutrient Agar | General bacteria | Peptone + extract base | Routine cultivation | Broader classical nutrient support |
| Tryptic Soy Agar | Broad routine microbiology | Casein + soy digest base | Clinical and environmental labs | Richer growth support |
| Plate Count Agar | Viable aerobic counts | Standard enumeration medium | Food and water testing | Widely standardized count medium |
Recommended Workflow
1. Prepare medium
Reconstitute, autoclave, and pour plates under aseptic conditions.
Reconstitute, autoclave, and pour plates under aseptic conditions.
2. Inoculate sample
Spread or streak according to the target workflow.
Spread or streak according to the target workflow.
3. Incubate
Use the organism-appropriate temperature and time window.
Use the organism-appropriate temperature and time window.
4. Read colonies
Assess count, growth quality, and morphology as needed.
Assess count, growth quality, and morphology as needed.
Strategic Use
Advantages
- Simple and easy-to-prepare medium
- Useful for teaching and routine microbiology
- Low complexity helps reduce background variables
Considerations
- Not selective for target groups
- Not ideal for very fastidious organisms
- No built-in differential indicator system
Selection Guide
- Choose when a simple non-selective agar is sufficient
- Suitable for non-fastidious routine cultivation
- Useful as a low-complexity alternative to richer agars