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Heart Infusion Agar | Nutrient‑Rich Medium for Fastidious Microorganisms | AS‑1246
$60.00 AUD
AuSaMicS Life Science • Enriched Agar Media
Heart Infusion Agar
Highly Nutritious Agar for Fastidious Microorganisms
Heart Infusion Agar is a rich, non-selective, general-purpose solid medium designed for the isolation and cultivation of fastidious microorganisms. It is widely used in clinical and research microbiology as a nutritive agar for delicate organisms and also serves as an excellent base for blood agar preparation when haemolytic reactions need to be observed.
AS-1246 pH 7.4 ± 0.2 ✓ Australian Stock 🧫 Fastidious Organisms 🩸 Blood Agar Base
Use: Fastidious organism isolation Type: Enriched, non-selective agar Documents: COA + SDS + TDS
Why Labs Use HI Agar
Rich nutrition for delicate organisms
Blood agar base for haemolysis work
General-purpose enriched solid medium
Classical formulation for clinical microbiology
Consistent quality for reliable plating
Typical Applications
Fastidious bacteria
Blood agar preparation
Haemolysis studies
Vaccine culture work
Blood agar preparation
Haemolysis studies
Vaccine culture work
⚠️ Important: Heart Infusion Agar vs Heart Infusion Broth
Heart Infusion Agar (AS-1246)
A solid enriched medium for colony isolation, cultivation, plate observation, and use as a blood agar base for haemolytic reactions. :contentReference[oaicite:1]{index=1}
A solid enriched medium for colony isolation, cultivation, plate observation, and use as a blood agar base for haemolytic reactions. :contentReference[oaicite:1]{index=1}
Heart Infusion Broth (AS-1247)
A liquid enriched medium better suited to propagation, suspension growth, and broth-based recovery or maintenance workflows.
A liquid enriched medium better suited to propagation, suspension growth, and broth-based recovery or maintenance workflows.
🔬 Technical Overview & Principle
Heart Infusion Agar is a highly nutritious, non-selective solid medium recommended for the isolation and cultivation of a broad variety of fastidious organisms. Published product sheets describe it as suitable for routine cultivation of demanding bacteria and note that it can also be used as a base for preparing blood agar for haemolysis determination. :contentReference[oaicite:2]{index=2}
Because the medium is rich but not inhibitory, it is useful where strong nutritional support is needed without selective agents or dyes. It has also been described as suitable for Vibrio cultivation, for supplement-based adaptations, and for mass cultivation of organisms in vaccine-related workflows. :contentReference[oaicite:3]{index=3}
Why this medium performs well:
Heart infusion provides rich soluble nutrients, tryptose contributes peptides and amino acids, sodium chloride maintains osmotic balance, and agar creates a firm surface for colony development and observation. This simple but nutrient-dense composition is why HI Agar remains a useful classical medium in clinical microbiology. :contentReference[oaicite:4]{index=4}
Heart infusion provides rich soluble nutrients, tryptose contributes peptides and amino acids, sodium chloride maintains osmotic balance, and agar creates a firm surface for colony development and observation. This simple but nutrient-dense composition is why HI Agar remains a useful classical medium in clinical microbiology. :contentReference[oaicite:4]{index=4}
Common use cases: isolation of fastidious organisms, preparation of blood agar, observation of haemolytic reactions, enriched plating for sensitive clinical isolates, and specialised supplemented workflows. :contentReference[oaicite:5]{index=5}
🧪 Typical Composition
🧫 Typical Laboratory Use Workflow
1
Prepare the medium
Reconstitute and sterilise according to the product instructions and validated laboratory method for enriched agar preparation.
Reconstitute and sterilise according to the product instructions and validated laboratory method for enriched agar preparation.
2
Use directly or enrich with blood
The medium may be used as an enriched non-selective agar on its own, or it may serve as a blood agar base for haemolytic reaction studies. :contentReference[oaicite:11]{index=11}
The medium may be used as an enriched non-selective agar on its own, or it may serve as a blood agar base for haemolytic reaction studies. :contentReference[oaicite:11]{index=11}
3
Inoculate and incubate
Inoculate with the target isolate or specimen and incubate under organism-appropriate conditions. Because the medium is non-selective, it is best suited to clean specimens or controlled workflows where broad recovery is desired.
Inoculate with the target isolate or specimen and incubate under organism-appropriate conditions. Because the medium is non-selective, it is best suited to clean specimens or controlled workflows where broad recovery is desired.
4
Observe colonies or haemolysis
Use the plate for colony isolation, fastidious organism recovery, or haemolytic pattern observation when prepared as blood agar.
Use the plate for colony isolation, fastidious organism recovery, or haemolytic pattern observation when prepared as blood agar.
📊 Comparative Enriched Solid Media
📌 Quick Specifications
Product: Heart Infusion Agar
Cat. No.: AS-1246
Format: Dehydrated culture medium
Type: Enriched, non-selective solid agar
Typical use: Fastidious organism isolation
Blood addition: Suitable as blood agar base
Special use: Haemolysis studies
Storage: 10–25°C, dry, tightly closed, protected from moisture and light
⭐ Key Advantages
✓Rich nutrient support for delicate organisms
✓Useful as a stand-alone enriched agar or blood agar base
✓Suitable for haemolytic reaction observation after blood addition
✓Classical medium for clinical, teaching, and research laboratories
✓Adaptable to supplemented workflows where needed
⚙️ Preparation Notes
Prepare according to the product instructions and your validated laboratory method.
For blood agar preparation, add sterile blood only after the basal medium has cooled to the appropriate temperature.
Because the medium is non-selective, good aseptic handling is important for reliable isolation performance.
🔗 Related AuSaMicS Products
Heart Infusion Broth
Liquid counterpart for propagation and enriched broth workflows
Brain Heart Infusion Agar
Broader enriched agar for maintenance and cultivation of fastidious microbes
Blood Agar Base Media
Complementary media for blood-enriched isolation workflows
Reliable Enriched Agar for Fastidious Organism Isolation
AuSaMicS supplies high-quality microbiological media designed for dependable performance in clinical, research, and industrial laboratories. Contact us for technical documentation, bulk supply, or method-aligned support.
For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
www.ausamics.com.au • support@ausamics.com • +61 412 520 598