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Heart Infusion Broth | Nutrient‑Rich Medium for Fastidious Microorganisms | AS‑1247
$55.00 AUD
AuSaMicS Life Science • Enriched Broth Media
Heart Infusion Broth
Highly Nutritious Broth for Fastidious Microorganisms
Heart Infusion Broth (HIB) is a rich, non-selective, general-purpose liquid medium formulated for the cultivation and propagation of fastidious microorganisms. It is widely used in blood culture work and related clinical microbiology applications, and is suitable for organisms such as streptococci, pneumococci, meningococci, and other nutritionally demanding bacteria.
AS-1247 pH 7.4 ± 0.2 ✓ Australian Stock 🧫 Fastidious Organisms 🩸 Blood Culture Work
Use: Fastidious bacterial propagation Type: Enriched, non-selective broth Documents: COA + SDS + TDS
Why Labs Use HIB
Rich nutrition for demanding organisms
Blood culture support in routine workflows
Well buffered for stable growth conditions
General-purpose clinical medium
Consistent quality for reproducible performance
Typical Organism Scope
Streptococci
Pneumococci
Meningococci
Other fastidious bacteria
Pneumococci
Meningococci
Other fastidious bacteria
⚠️ Important: Heart Infusion Broth vs Brain Heart Infusion Broth
Heart Infusion Broth (AS-1247)
A classic, highly nutritious broth based on heart infusion, intended for cultivation of a wide range of fastidious organisms and commonly recommended for blood culture work.
A classic, highly nutritious broth based on heart infusion, intended for cultivation of a wide range of fastidious organisms and commonly recommended for blood culture work.
Brain Heart Infusion Broth (BHI)
A related but distinct enriched medium containing both brain and heart infusions, often described as even broader in scope for fastidious and non-fastidious organisms, including many aerobic and anaerobic bacteria.
A related but distinct enriched medium containing both brain and heart infusions, often described as even broader in scope for fastidious and non-fastidious organisms, including many aerobic and anaerobic bacteria.
🔬 Technical Overview & Principle
Heart Infusion Broth is a non-selective enriched liquid medium developed to support organisms with demanding nutritional requirements. Official product literature describes it as a highly nutritious and well-buffered medium that supports growth of a wide variety of organisms, particularly fastidious bacteria. :contentReference[oaicite:1]{index=1}
Historically, infusion media derived from animal tissue were among the earliest rich media used in bacteriology, and modern Heart Infusion Broth continues that role by providing a concentrated nutrient environment for the propagation and maintenance of clinical isolates and other delicate organisms. :contentReference[oaicite:2]{index=2}
Why this medium performs well:
Heart infusion contributes soluble growth factors and organic nutrients, while the broth system provides a uniform, well-buffered liquid environment suitable for recovery, propagation, and routine cultivation of fastidious organisms.
Heart infusion contributes soluble growth factors and organic nutrients, while the broth system provides a uniform, well-buffered liquid environment suitable for recovery, propagation, and routine cultivation of fastidious organisms.
Common use cases: blood culture-related work, propagation of pathogenic cocci, maintenance of fastidious organisms, and general enriched broth cultivation in clinical and research laboratories. :contentReference[oaicite:3]{index=3}
🧪 Typical Functional Composition
🧫 Typical Laboratory Use Workflow
1
Prepare the broth
Reconstitute the dehydrated medium according to your validated laboratory method and sterilise as required for broth media preparation.
Reconstitute the dehydrated medium according to your validated laboratory method and sterilise as required for broth media preparation.
2
Inoculate the specimen or culture
Use aseptic technique to inoculate with the target isolate or clinical material where an enriched, non-selective broth is appropriate.
Use aseptic technique to inoculate with the target isolate or clinical material where an enriched, non-selective broth is appropriate.
3
Incubate under appropriate conditions
Incubation temperature, atmosphere, and duration depend on the organism and laboratory protocol. The broth is especially suited to fastidious organisms requiring enriched nutrition. :contentReference[oaicite:8]{index=8}
Incubation temperature, atmosphere, and duration depend on the organism and laboratory protocol. The broth is especially suited to fastidious organisms requiring enriched nutrition. :contentReference[oaicite:8]{index=8}
4
Use for propagation or downstream testing
Following growth, the broth culture may be used for subculture, maintenance, or other downstream microbiological procedures consistent with validated methods.
Following growth, the broth culture may be used for subculture, maintenance, or other downstream microbiological procedures consistent with validated methods.
📊 Comparative Enriched Broth Media
📌 Quick Specifications
Product: Heart Infusion Broth
Cat. No.: AS-1247
Format: Dehydrated culture medium
Type: Enriched, non-selective liquid broth
Typical organisms: Streptococci, pneumococci, meningococci, other fastidious bacteria
Typical application: Blood culture work and allied investigations
Medium class: General-purpose enriched broth
Storage: 10–25°C, dry, tightly closed, protected from moisture and light
⭐ Key Advantages
✓Highly nutritious for delicate or demanding bacteria
✓Useful in blood culture-related workflows
✓Well-buffered for dependable broth performance
✓Good for propagation, maintenance, and recovery applications
✓Suitable for clinical, educational, and research laboratories
⚙️ Preparation Notes
Prepare according to the product instructions and your validated laboratory procedure.
Because it is a non-selective enriched broth, sterility and good aseptic handling are important for reliable results.
Incubation conditions should be selected based on the target organism and intended use.
🔗 Related AuSaMicS Products
Brain Heart Infusion Broth
Broader enriched broth for fastidious and general microbiology use
Tryptic Soy Broth
Routine general-purpose broth for broad laboratory use
Blood Agar Base Media
Complementary solid media for fastidious organism isolation
Reliable Enriched Broth for Fastidious Organism Recovery
AuSaMicS supplies high-quality microbiological media designed for dependable performance in clinical, research, and industrial laboratories. Contact us for bulk supply, technical documentation, or method-aligned support.
For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
www.ausamics.com.au • support@ausamics.com • +61 412 520 598