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King’s B Medium Base (AS-1260) | Pseudomonas Agar F for Fluorescein
$39.00 AUD
Documentation
AuSaMicS Life Science • Diagnostic Pigmentation Media
King's B Medium Base
Pseudomonas Agar F / Flo Agar
A specialized differential medium designed to enhance the production of fluorescein (pyoverdin). A critical tool for the definitive identification of Pseudomonas species in clinical and environmental samples.
AS-1260 ✨ UV Fluorescent ✓ USP/EP Compliant 🧬 Pigment Enhancement
Target: Pyoverdin Production Incubation: 25-30°C pH: 7.2 ± 0.2
Diagnostic Performance
Fluorescein (+)
Yellow-Green Pigment
Yellow-Green Pigment
UV Visualization
Bright Fluorescence at 366nm
Bright Fluorescence at 366nm
Optimized Peptones
Rich in Phosphorus for Pigment
Rich in Phosphorus for Pigment
🔬 Key Applications
Clinical Diagnostics
Differentiation of Pseudomonas aeruginosa from other non-fermenting Gram-negative bacilli.
Pharma Quality Control
Validated for use in USP/EP microbial limit tests for the detection of P. aeruginosa.
🧪 Nutrient Formulation (per Liter)
| Ingredient | Concentration | Function |
|---|---|---|
| Peptone Mixture | 20.0 g | Amino Acids (High Phosphorous) |
| Dipotassium Phosphate | 1.5 g | Phosphorous Source for Pigment |
| Magnesium Sulfate | 1.5 g | Cation Cofactor for Fluorescein |
| Bacteriological Agar | 15.0 g | Solidifying Agent |
*Note: Requires addition of 10-15 mL/L of Glycerol (not included in base powder).
📋 Technical Specs
| Catalog No. | AS-1260 |
| Final pH | 7.2 ± 0.2 |
| Yield | 38.0 g / L |
| Appearance | Light Amber (Clear) |
⚗️ Laboratory Prep
- 1 Suspend 38.0g in 1L purified water. Add 10-15mL Glycerol.
- 2 Heat to boiling to dissolve agar completely.
- 3 Autoclave at 121°C for 15 minutes.
- 4 Cool to 45°C and pour into sterile plates. Store at 2-8°C.
Technical Data Sheet Summary
Document: TDS-AS-1260 — full Technical Data Sheet available as a downloadable Word/PDF document. Summary specifications below.
Identity
| Product Name | King's B Medium Base |
| Catalog Number | AS-1260 |
| Medium Type | Differential, dehydrated (peptone-based) |
| Weighed Quantity | 38.0 g/L + 10–15 mL/L glycerol |
| Final pH (25°C) | 7.2 ± 0.2 |
Quality Control (Fluorescence / Growth Promotion Testing)
| Test Organism | Inoculum | Expected Result (24–48 h, 25–30°C) |
|---|---|---|
| Pseudomonas aeruginosa ATCC 27853 | ≤ 100 CFU | Good growth; bright yellow-green fluorescence under UV (366 nm) |
| Pseudomonas fluorescens ATCC 13525 | ≤ 100 CFU | Good growth; fluorescence expected (non-specific for species ID) |
| Escherichia coli ATCC 25922 | ≤ 100 CFU | Growth permitted; no fluorescence (differential check) |
| Staphylococcus aureus ATCC 25923 | ≤ 100 CFU | Growth permitted to inhibited; no fluorescence |
Principle & Mode of Action
Fluorescein (pyoverdin) is a siderophore synthesised by fluorescent pseudomonads under iron-limited conditions. King's B Medium supplies phosphorus-rich peptone and magnesium sulfate to activate this biosynthetic pathway while glycerol provides the carbon source, making pigment expression and UV fluorescence a reliable, medium-driven phenotypic marker rather than a strain-specific coincidence.
Historical Development
Described by King, Ward, and Raney (1954) alongside the complementary pyocyanin-enhancing King A Medium; codified by the U.S. FDA as BAM Media M128 for fluorescein production testing.
Literature / References
- King, E.O., Ward, M.K., & Raney, D.E. (1954). Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine, 44(2), 301–307.
- U.S. Food and Drug Administration. Bacteriological Analytical Manual (BAM), Media M128 — Pseudomonas Agar F (for fluorescein production).
- International Organization for Standardization. ISO 16266 — Water quality: detection and enumeration of Pseudomonas aeruginosa.
Full Technical Data Sheet — AS-1260Word/PDF document with complete QC methods and references
Download TDS
Safety Data Sheet Summary
Document: SDS-AS-1260 — full 16-section GHS-compliant Safety Data Sheet (Australian regulatory format) available as a downloadable Word/PDF document.
Product & Company Identification
| Product Name | King's B Medium Base |
| Catalog Number | AS-1260 |
| Recommended Use | Laboratory differential culture medium; not for diagnostic use without confirmatory testing, and not for human/animal administration |
| Manufacturer | AuSaMicS Pty Ltd, Melbourne, Victoria, Australia |
Hazard Overview
Dehydrated culture media of this type are generally regarded as having low acute hazard, but the full SDS should always be consulted before handling. The dry powder may cause mechanical/dust irritation to eyes, skin, and the respiratory tract; the prepared (autoclaved) gel presents minimal chemical hazard. Once inoculated and incubated, standard microbiological biosafety practices apply.
- Physical form: Dehydrated powder — minimise dust generation during weighing and reconstitution
- Biological hazard: Not a biological hazard as supplied; becomes a potential biohazard only after inoculation with microorganisms
First Aid & Handling Highlights
- Eye contact: Rinse cautiously with water for several minutes
- Skin contact: Wash with soap and water
- Inhalation: Remove to fresh air; avoid generating dust during handling
- Handling: Use appropriate PPE (gloves, safety glasses); weigh with local exhaust ventilation where practical; autoclave inoculated media before disposal
This is a summary for quick reference only. Always consult and follow the complete, current Safety Data Sheet before use, handling, storage, or disposal — particularly after inoculation with clinical or environmental isolates, where standard biosafety practices apply.
Full Safety Data Sheet — AS-126016-section GHS-compliant SDS, Australian format, with pictograms
Download SDS
Certificate of Analysis
Each batch of AS-1260 is supplied with a batch-specific Certificate of Analysis confirming compliance with the specifications and fluorescence performance results below.
| Test | Specification | Typical Result |
|---|---|---|
| Appearance (dehydrated) | Free-flowing powder | Conforms |
| Appearance (prepared) | Light amber, clear | Conforms |
| Final pH (25°C) | 7.2 ± 0.2 | Reported per batch |
| Fluorescence — P. aeruginosa ATCC 27853 | Positive (bright yellow-green, 366 nm) | Reported per batch |
| Negative control — E. coli ATCC 25922 | No fluorescence | Reported per batch |
Batch Traceability
Batch/lot number, manufacture date, and re-test/expiry date are printed on the product label and the corresponding Certificate of Analysis issued with each shipment. Quote your batch number when requesting a COA reprint.
Request Batch-Specific COA — AS-1260Provide your batch/lot number to receive the matching Certificate of Analysis
Request COA