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LB Broth (Miller) (AS-1270) – High-Salt Medium for Bacterial Culture
$29.00 AUD
LB Broth (Miller)
Luria-Bertani Broth, Miller Modification — General-Purpose Liquid Culture Medium
Catalogue No: AS-1270
Luria-Bertani Broth, Miller Modification — General-Purpose Liquid Culture Medium
Catalogue No: AS-1270
E. coli & Molecular Biology
Miller Formulation
NaCl 10 g/L
Liquid Culture
Australian Made
QC Certified
Overview
LB Broth (Miller), Luria-Bertani Broth Miller modification, is a nutrient-rich, general-purpose liquid culture medium widely used for the cultivation and maintenance of Escherichia coli in molecular biology and recombinant DNA applications. The Miller modification contains a higher sodium chloride concentration (10.0 g/L) supporting osmotic balance and membrane transport across standard E. coli strains.
The combination of tryptone and yeast extract supplies essential amino acids, peptides, vitamins, and growth factors, supporting robust growth including K-12 derivatives. LB Broth (Miller) is used as both a liquid medium and as a base for LB Agar (Miller) plate preparation.
Composition (per litre)
| Component | CAS Number | Function | Amount |
|---|---|---|---|
| Tryptone | 73049-73-7 | Nitrogen, carbon, amino acids | 10.0 g |
| Yeast Extract | 8013-01-2 | B-vitamins, growth factors | 5.0 g |
| Sodium Chloride | 7647-14-5 | Osmotic balance — Miller high-salt | 10.0 g |
| Final pH | 7.0 ± 0.2 at 25 °C | ||
LB Broth Formulation Comparison
| Component | Miller (AS-1270) | Lennox (AS-1269) | Bertani |
|---|---|---|---|
| Tryptone | 10.0 g/L | 10.0 g/L | 10.0 g/L |
| Yeast Extract | 5.0 g/L | 5.0 g/L | 5.0 g/L |
| NaCl | 10.0 g/L | 5.0 g/L | 0 g/L |
| Osmolarity | ~310 mOsm/kg | ~170 mOsm/kg | ~60 mOsm/kg |
| Best for | Standard E. coli — global default | Salt-sensitive strains | Very low osmolarity |
Key Features
- Standard Miller formulation — global default for routine E. coli liquid culture
- Optimised NaCl (10.0 g/L) for osmotic balance and membrane transport
- Supports rapid growth to high OD₆₀₀ for overnight and log-phase cultures
- Suitable as liquid medium and as base for LB Agar (Miller) preparation
- Easily modified with antibiotics, IPTG, sugars, or other supplements
- Compatible with all standard E. coli K-12 and B strains
Applications
- Routine Liquid Culture: Overnight cultures for miniprep, midiprep, maxiprep; mid-log phase for transformation
- Competent Cell Preparation: Chemical (CaCl₂) and electrocompetent E. coli preparation
- Protein Expression: Pre-induction liquid culture for BL21, Rosetta, ArcticExpress
- Plasmid Maintenance: Propagation and amplification of plasmid-bearing strains
- Agar Base: Add 15.0 g/L agar before autoclaving to prepare LB Agar (Miller)
- Teaching: Standard molecular biology liquid culture instruction
Preparation
- Dissolve 25.0 g in 1 litre of demineralised or purified water.
- Mix or heat gently with agitation until fully dissolved.
- Adjust pH to 7.0 ± 0.2 if required.
- Sterilise by autoclaving at 121 °C for 15 minutes.
- Cool to room temperature before use or inoculation.
- Add filter-sterilised antibiotics or supplements aseptically as required.
For LB Agar (Miller): Add 15.0 g/L agar before autoclaving. Cool to 45–50 °C, add antibiotics, pour 20–25 mL per 90 mm plate.
Common Antibiotic Supplements
| Antibiotic | Working Concentration | Application |
|---|---|---|
| Ampicillin | 100 µg/mL | Amp-resistant plasmid selection |
| Kanamycin | 50 µg/mL | Kan-resistant plasmid selection |
| Chloramphenicol | 25 µg/mL | Cm-resistant selection |
| Tetracycline | 12.5 µg/mL | Tet-resistant selection |
| Gentamicin | 10 µg/mL | Gm-resistant selection |
| Spectinomycin | 50 µg/mL | Spec-resistant selection |
Storage
| Dehydrated powder | 15–30 °C, tightly closed, dry container |
| Prepared broth | 2–8 °C, use within 1 week |
| Protect from | Moisture, light, and extreme temperatures |
| Do not use if | Contamination, precipitation, turbidity, or pH shift observed |
For laboratory, molecular biology, and educational use only. Not for medical, food, or veterinary applications. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Identification
| Product Name | LB Broth (Miller) — Luria-Bertani Broth, Miller Modification |
| Catalogue Number | AS-1270 |
| Also known as | LB Medium (Miller), Luria Broth (Miller), LB-Miller |
| HS Code | 3821.00.00 |
| Physical Form | Fine homogeneous powder |
| Colour (powder) | Light beige to cream |
| Physical state (prepared) | Clear to slightly turbid light amber liquid |
| Original Reference | Miller J.H. (1972) Experiments in Molecular Genetics, CSHL |
| ATCC Medium | ATCC Medium 1065 |
Composition (per litre)
| Component | CAS Number | Function | Amount |
|---|---|---|---|
| Tryptone | 73049-73-7 | Nitrogen, carbon, amino acids, peptides | 10.0 g |
| Yeast Extract | 8013-01-2 | B-vitamins, growth factors, nucleotides | 5.0 g |
| Sodium Chloride | 7647-14-5 | Osmotic balance — Miller high-salt | 10.0 g |
Total: 25.0 g/L | pH: 7.0 ± 0.2 at 25 °C | Osmolarity: ~310 mOsm/kg
Physical & Chemical Specifications
| pH (prepared, 25 °C) | 7.0 ± 0.2 |
| Moisture (loss on drying) | ≤ 5.0% |
| Solubility | Readily and completely soluble in purified water |
| NaCl concentration | 10.0 g/L — Miller high-salt formulation |
| Osmolarity (approx.) | ~310 mOsm/kg |
| Selectivity | Non-selective |
Performance Characteristics
| Organism | ATCC No. | Expected Result | Incubation |
|---|---|---|---|
| E. coli K-12 | 23716 | Good growth — turbid broth | 37 °C, shaking, 18–24 h |
| E. coli DH5α | N/A | Good growth — cloning host | 37 °C, shaking, 12–16 h |
| E. coli BL21(DE3) | N/A | Good growth — expression host | 37 °C, shaking, 3–4 h to OD 0.6 |
| E. coli TOP10 | N/A | Good growth — cloning host | 37 °C, shaking, 12–16 h |
| Salmonella typhimurium | 14028 | Good growth | 37 °C, shaking, 12–16 h |
Quality Standards
| Original Reference | Miller J.H. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory |
| Standard References | Sambrook & Russell — Molecular Cloning; Current Protocols in Molecular Biology |
| ATCC Medium | ATCC Medium 1065 |
| Country of Manufacture | Australia |
For laboratory and research use only. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
GHS Classification: Not classified as hazardous (AU WHS Regulations 2011) | Signal Word: None | ADG: Not regulated
Section 1 — Identification
| Product Name | LB Broth (Miller) — Luria-Bertani Broth, Miller Modification |
| Catalogue Number | AS-1270 |
| Intended Use | Non-selective liquid culture medium — laboratory research, molecular biology, and educational use only |
| Supplier | AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 |
| ABN | 56 676 640 467 |
| Emergency Phone | +61 412 520 598 |
| support@ausamics.com.au |
Section 2 — Hazard Identification
Not classified as hazardous according to GHS criteria (AU WHS Regulations 2011). No hazard pictograms, signal word, or hazard statements required. Standard laboratory precautions apply.
Section 3 — Composition
| Component | CAS Number | Proportion | Classification |
|---|---|---|---|
| Tryptone | 73049-73-7 | ~40% | Not classified |
| Yeast Extract | 8013-01-2 | ~20% | Not classified |
| Sodium Chloride | 7647-14-5 | ~40% | Not classified |
Section 4 — First Aid
| Inhalation | Remove to fresh air. Seek medical advice if symptoms persist. |
| Skin Contact | Wash thoroughly with soap and water. |
| Eye Contact | Flush with water for at least 15 minutes. Seek medical advice if irritation persists. |
| Ingestion | Rinse mouth. Seek medical advice if large quantities ingested. |
Section 7 — Handling & Storage
| Handling | Standard lab precautions. Avoid dust. Wash hands after use. |
| Storage (powder) | 15–30 °C, tightly closed, dry, away from moisture and light |
| Storage (prepared broth) | 2–8 °C, use within 1 week |
Section 8 — Personal Protection
| Respiratory | Dust mask (P1) if handling in poorly ventilated area |
| Eye protection | Safety glasses recommended |
| Gloves | Disposable nitrile or latex laboratory gloves |
Section 13 — Disposal
Autoclave inoculated liquid cultures (121 °C, 30 min) before disposal. Dispose of unused powder as general laboratory waste in accordance with local regulations.
Section 14 — Transport
Not classified as dangerous goods under ADG, IATA, or IMDG. No special transport requirements.
⬇ Download Full SDS — 16 Sections (PDF)Prepared in accordance with Australian WHS Regulations and GHS 7th Edition. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au | Version 1.0 — March 2026
Product Information
| Product Name | LB Broth (Miller) — Luria-Bertani Broth, Miller Modification |
| Catalogue Number | AS-1270 |
| Lot / Batch Number | Contact us for batch-specific COA |
| Manufacturing Date | Available on request |
| Expiry Date | Available on request |
| Storage Conditions | 15–30 °C, tightly closed, dry |
Specification & Test Results
| Test | Specification | Typical Result | Status |
|---|---|---|---|
| Appearance (powder) | Light beige to cream fine powder | Complies | ✓ PASS |
| Appearance (prepared) | Clear to slightly turbid light amber | Complies | ✓ PASS |
| pH (prepared, 25 °C) | 7.0 ± 0.2 | 7.0 | ✓ PASS |
| Moisture (loss on drying) | ≤ 5.0% | Complies | ✓ PASS |
| Solubility | Completely soluble in purified water | Complies | ✓ PASS |
| Sterility (prepared medium) | No microbial growth | Complies | ✓ PASS |
| E. coli K-12 ATCC 23716 | Good growth — turbid broth | Complies | ✓ PASS |
| NaCl content (Miller) | 10.0 ± 0.3 g/L | Complies | ✓ PASS |
Authorisation
| Released by | Hassan Salimi |
| Title | Founder and Technical Director |
| Organisation | AuSaMicS Pty Ltd |
Need a batch-specific COA?
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
⬇ Download Certificate of Analysis (PDF)
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
This Certificate of Analysis is issued by AuSaMicS Pty Ltd and is valid for the batch identified above. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au