LB Broth (Miller)

LB Broth, Miller

Luria-Bertani Broth (Miller Modification)
Catalog Number: AS-1270

Product Information

• Catalog Number: AS-1270

• Form: Dehydrated powder

• pH (prepared): 7.0 ± 0.2 at 25 °C

• HS Tariff Code: 3821.00 — prepared culture media for development of microorganisms

Typical Composition (per liter)

• Tryptone – 10.0 g

• Yeast Extract – 5.0 g

• Sodium Chloride – 10.0 g

Overview

LB Broth, Miller modification is a nutrient-rich, general-purpose culture medium widely used for the cultivation and maintenance of Escherichia coli, particularly in molecular biology and recombinant DNA applications. It is a modification of the original Luria-Bertani formulation with a higher sodium chloride concentration, which supports osmotic balance and membrane transport.

The combination of tryptone and yeast extract supplies essential amino acids, peptides, vitamins, and growth factors, supporting robust growth of E. coli strains, including K-12 derivatives with auxotrophic requirements. LB Broth, Miller is commonly used as both a liquid medium and as a base for LB agar preparation.

Applications

• Routine cultivation of Escherichia coli

• Molecular biology and recombinant DNA work

• Plasmid maintenance and protein expression

• Preparation of competent cells

• Microbial fermentation and research laboratories

Key Features

• Standard Miller-formulation LB medium

• Optimised sodium chloride concentration

• Supports rapid and reproducible E. coli growth

• Easily modified with supplements (e.g. antibiotics, sugars)

• Suitable for liquid culture or agar preparation

Storage & Handling

• Dehydrated powder: Store at 15–30 °C in a dry, sealed container

• Prepared medium: Store at 2–8 °C

• Protect from moisture and light

• Use prepared medium within recommended laboratory timeframes

Intended Use

For laboratory use only.
Not for medical, food, or veterinary applications.

Quality & Compliance

• Manufactured under controlled conditions

• Consistent batch-to-batch performance

• COA and SDS available upon request