Liver Infusion Broth

Liver Infusion Broth

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Product Overview

Liver Infusion Broth (AS-1276) is one of the richest liquid enrichment media available in clinical and veterinary microbiology. It serves as the traditional gold-standard broth for cultivating extremely fastidious and oxygen-sensitive organisms that fail to grow in ordinary peptone media. The medium derives its exceptional nutritive value from a concentrated liver tissue infusion, naturally contributing hemin, vitamin K, reducing substances, essential growth factors, and long-chain fatty acids — creating an environment ideal for strict anaerobes and slow-growing bacterial pathogens.

The inherently low oxidation-reduction potential (Eh) renders this medium ideal for oxygen-sensitive organisms without mandatory supplementation. The deliberate absence of added carbohydrates prevents accumulation of inhibitory organic acids during extended incubation of up to 21 days.

Applications

  • Primary enrichment of Brucella spp. from blood, bone marrow, and tissue specimens
  • Cultivation of Francisella tularensis (follow applicable BSL-3 containment regulations)
  • Growth of strict anaerobes: Clostridium, Bacteroides, Fusobacterium, Actinomyces spp.
  • Resuscitation of heat-injured or freeze-injured bacterial cells from clinical, food, and environmental samples
  • Liquid phase in biphasic blood culture systems (Castaneda bottle design)
  • Base medium for supplementation with hemin, vitamin K, serum, or other enrichments

Key Features

  • Natural liver infusion solids — hemin, vitamin K, NAD, reducing compounds, and uncharacterised growth factors
  • Very low Eh — inherently ideal for strict anaerobes; further reduced by optional cysteine/thioglycollate supplementation
  • No added carbohydrate — eliminates acid-mediated inhibition of slow-growing pathogens
  • Flexible formulation — compatible with serum, cysteine, thioglycollate, hemin, and vitamin K additions

Product Identification

Product Name Liver Infusion Broth
Product Code AS-1276
Grade Microbiological / Culture Media Grade
Format Dehydrated powder, beige to light tan
Quantity 500 g per container
Storage 15–30°C, dry, dark, tightly sealed
Shelf Life 3 years from manufacture date
Intended Use Professional microbiological laboratory use only

Composition (per litre prepared medium)

Ingredient Quantity (g/L) Function
Liver Infusion Solids (from 250–500 g fresh liver) 8.0–12.0 Hemin, vitamin K, NAD, reducing factors, growth factors
Proteose Peptone / Tryptose 10.0 Nitrogen and carbon source; peptides; amino acids
Sodium Chloride (NaCl) 5.0 Osmotic balance
Approximate total solids 23.0–27.0 —

Preparation

Suspend 23.0–27.0 g dehydrated medium in 1 litre purified water. Mix thoroughly until dissolved. Dispense as required. Autoclave at 121°C for 15 minutes. Cool to 50–55°C before adding any heat-labile supplements (cysteine, serum, hemin). Final pH: 7.2 ± 0.2 at 25°C.

Incubation

Temperature 35–37°C
Anaerobes Strict anaerobic atmosphere (< 0.1% O₂)
Brucella / Francisella 5–10% CO₂ (capneic incubation)
Duration 48 hours – 21 days depending on organism
Download Product Description (PDF)
Disclaimer: This product is for professional microbiological laboratory use only. For the most current version of this document, visit www.ausamics.com.au. AuSaMicS Pty Ltd reserves the right to modify specifications without prior notice.

Physical & Chemical Properties

Catalogue Number AS-1276
Synonyms Liver Broth; Liver Extract Broth
Colour (powder) Beige to light tan, homogeneous
Colour (prepared) Light amber
Clarity (prepared) Clear to slightly hazy
Odour Characteristic faint meat/infusion odour
pH (25°C, prepared) 7.0–7.4
Oxidation-Reduction Potential (Eh) ≤ −50 mV; < −150 mV with 0.1% cysteine supplement
Solubility Freely soluble in purified water (23–27 g/L)

Microbiological Performance

Organism ATCC No. Expected Result Incubation
Clostridium sporogenes 11437 Good growth (turbidity) 48–72 h, 37°C, anaerobic
Bacteroides fragilis 25285 Good growth (turbidity) 48 h, 37°C, strict anaerobic
Brucella abortus 4315 Growth within 3–5 days 5 days, 37°C, 5–10% CO₂
Fusobacterium necrophorum 25286 Good growth 48–72 h, 37°C, anaerobic
Uninoculated control — No turbidity 48 h, 37°C, aerobic

Preparation Protocol

Step Action Parameter
1 Weigh dehydrated medium 23.0–27.0 g per litre
2 Dissolve in purified water (Type I or II) 1 litre; stir until homogeneous
3 Dispense into containers Tubes or bottles as required
4 Sterilise by autoclaving 121°C / 15 min
5 Cool before supplementing ≤ 50–55°C prior to adding heat-labile supplements
6 (optional) Supplement aseptically 0.1% cysteine or thioglycollate; 5–10% serum; hemin 5 µg/mL + vitamin K1 1 µg/mL

Storage & Stability

Powder — storage 15–30°C, dry, dark, original sealed container
Powder — shelf life 3 years from manufacture date
Prepared medium 2–8°C, protected from light, use within 3 months
Do not use if Powder is caked or clumped; prepared medium is turbid, precipitated, or discoloured
Download TDS (PDF)
Disclaimer: This technical data sheet is for professional laboratory reference only. AuSaMicS Pty Ltd reserves the right to update specifications without prior notice. Visit www.ausamics.com.au for the latest version.

Section 1 — Identification

Product Name Liver Infusion Broth
Product Code AS-1276
Supplier AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 | ABN: 56 676 640 467
Phone +61 412 520 598
Email support@ausamics.com
Emergency Poisons Information Centre (Australia): 13 11 26 (24 h)

Section 2 — Hazard Identification

GHS Classification: NOT classified as a hazardous substance (dehydrated powder form, normal conditions of use).

Precautionary notes (not GHS hazards):

  • Contains animal-derived liver infusion — apply institutional biosafety policies for animal-origin material
  • Organisms cultivated may be BSL-2 or BSL-3 pathogens — comply with national and institutional biosafety regulations
  • Treat all prepared/inoculated media as potentially infectious

Section 3 — Composition

Component Content (g/L) CAS Number GHS Classification
Liver Infusion Solids 8.0–12.0 Not assigned (biological extract) Not classified
Proteose Peptone / Tryptose 10.0 Not assigned (complex biological) Not classified
Sodium Chloride 5.0 7647-14-5 Not classified at use concentration

Section 4 — First Aid

Inhalation Remove to fresh air. Seek medical attention if symptoms persist.
Skin Contact Wash with soap and water. Remove contaminated clothing. Seek medical attention if irritation develops.
Eye Contact Flush immediately with water for ≥ 15 minutes holding eyelids open. Seek immediate medical attention.
Ingestion Do not induce vomiting. Rinse mouth with water. Seek medical attention. Show this SDS to medical staff.

Section 7 — Handling & Storage

Handling Use in well-ventilated laboratory. Avoid dust inhalation and contact with eyes, skin, clothing. Follow GLP.
Powder storage 15–30°C, original tightly sealed container, away from moisture, heat, and direct light
Prepared medium 2–8°C, protected from light, use within 3 months

Section 8 — PPE

PPE Type Specification
Gloves Nitrile or latex; double-glove for BSL-3 organisms
Eye / Face Safety glasses or goggles; face shield when aerosol risk
Body Laboratory coat; impervious gown for culture work
Respiratory Not required for routine powder handling; P2/N95 for bulk weighing

Section 13 — Disposal

  • Unused powder: Dispose as general laboratory waste per local council and state/territory regulations.
  • Prepared / inoculated medium: Autoclave at 121°C / 30 min (gravity cycle) before disposal as sterilised biological waste.
  • Packaging: Rinse container; dispose per local regulations.

Section 14 — Transport

Not regulated as a dangerous good (ADG / IATA / IMDG) in dehydrated powder form. Protect from moisture during transport.

Download Full SDS (PDF)
Disclaimer: This SDS has been prepared in accordance with GHS (Rev. 7) and Safe Work Australia codes. Information applies to the dehydrated product form only. Prepared and inoculated media must be treated as potentially infectious. AuSaMicS Pty Ltd | support@ausamics.com | +61 412 520 598 | ABN: 56 676 640 467.

Batch Identification

Product Name Liver Infusion Broth
Product Code AS-1276
Batch / Lot No. LIB-2026-001
Manufacture Date March 2026
Expiry Date March 2029
Quantity Released 500 g

Physicochemical Testing

Test Parameter Specification Result Status
Appearance (powder) Beige to light tan, homogeneous Beige, homogeneous, free-flowing ✓ Pass
pH (25°C, 23–27 g/L) 7.0–7.4 7.2 ✓ Pass
Appearance (prepared) Light amber, clear to slightly hazy Light amber, clear ✓ Pass
Solubility Completely soluble in purified water Complete dissolution ✓ Pass
Loss on Drying ≤ 5.0% 3.1% ✓ Pass

Microbiological Performance

Organism ATCC No. Specification Result Status
Clostridium sporogenes 11437 Growth within 48–72 h (anaerobic) Good growth at 48 h ✓ Pass
Bacteroides fragilis 25285 Good growth within 48 h (strict anaerobic) Good growth at 48 h ✓ Pass
Brucella abortus 4315 Growth within 3–5 days (5–10% CO₂) Growth at day 4 ✓ Pass
Uninoculated control — No growth at 48 h No growth ✓ Pass

Sterility Testing

Method USP <71> Sterility — Membrane filtration / Direct inoculation
Incubation 14 days at 20–25°C (FTM) and 30–35°C (SCDM)
Result No growth observed
Conclusion ✓ PASS

Release Conclusion

✓ BATCH RELEASED — ALL TESTS PASSED
Lot No. LIB-2026-001 meets all physicochemical, microbiological performance, and sterility specifications.

Download COA (PDF)
Disclaimer: This Certificate of Analysis is issued by AuSaMicS Pty Ltd for the specific batch indicated. Results apply only to the tested batch. AuSaMicS Pty Ltd shall not be liable for results arising from improper storage, handling, or use of this product. ABN: 56 676 640 467 | support@ausamics.com | www.ausamics.com.au
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