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Lysine Iron Agar (LIA) | Differential Medium for Enteric Pathogens | AS-1279
Lysine Iron Agar (LIA) | Differential Medium for Enteric Pathogens | AS-1279
$95.00 AUD
AuSaMicS Life Science • Differential Enteric Media
Lysine Iron Agar (LIA)
Differential Medium for Enterobacteriaceae & Enteric Pathogens
Diagnostic agar for differentiation of enteric bacteria based on lysine decarboxylation, lysine deamination, and hydrogen sulfide production. Widely used in Salmonella and enteric identification workflows.
AS-1279
🧫 Differential Medium
✓ Australian Stock
🧪 H₂S / Lysine Reactions
Workflow: Enteric identification
Format: Dehydrated culture medium
Support: COA / SDS / TDS available
Recommended Pack Sizes
| 100 g | AUD 45 |
| 250 g | AUD 89 |
| 500 g | AUD 145 |
| 1 kg | AUD 239 |
| 5 kg | AUD 925 |
Pricing strategy: 500 g positioned as best-seller, 1 kg as best-value for QC and routine lab use.
Overview
Lysine Iron Agar (LIA) is a differential medium used for the characterization of enteric bacteria on the basis of three key biochemical reactions: lysine decarboxylation, lysine deamination, and hydrogen sulfide generation. It is commonly used as part of enteric identification schemes, especially for differentiation of Salmonella, Proteus, Providencia, Citrobacter, and related Enterobacteriaceae.
The medium combines lysine, glucose, ferric ammonium citrate, sodium thiosulfate, and bromcresol purple in an agar slant/deep format to provide clear reaction patterns. LIA is often used alongside TSI, urea, citrate, and motility media in routine microbiology workflows.
Applications
- Differentiation of enteric bacteria based on lysine reactions and H₂S production
- Supplementary identification of Salmonella and related enteric pathogens
- Routine use in food, environmental, water, and clinical microbiology laboratories
- Use in enteric screening panels alongside TSI, citrate, urease, and motility tests
- Teaching, research, and QC microbiology applications
Key Features
Lysine decarboxylation
Supports detection of alkaline butt reactions after glucose utilization.
Supports detection of alkaline butt reactions after glucose utilization.
Lysine deamination
Characteristic slant reactions aid differentiation of Proteus-Providencia-Morganella group members.
Characteristic slant reactions aid differentiation of Proteus-Providencia-Morganella group members.
H₂S indication
Ferric ammonium citrate and sodium thiosulfate permit blackening in positive organisms.
Ferric ammonium citrate and sodium thiosulfate permit blackening in positive organisms.
Enteric workflow fit
Designed for slant/deep biochemical interpretation in routine identification panels.
Designed for slant/deep biochemical interpretation in routine identification panels.
Typical Composition (per Litre)
| Ingredient | Typical Amount | Function |
|---|---|---|
| Peptone / balanced peptone | 5.0 g | General nutrient support |
| Yeast Extract | 3.0 g | Vitamins and growth factors |
| Glucose | 1.0 g | Fermentable carbohydrate |
| L-Lysine | 10.0 g | Decarboxylation / deamination substrate |
| Ferric Ammonium Citrate | 0.5 g | H₂S indicator component |
| Sodium Thiosulfate | 0.04 g | Sulfur source for H₂S production |
| Bromcresol Purple | 0.02 g | pH indicator |
| Agar | 12.0–15.0 g | Solidifying agent |
Reconstitution guide for commercial LIA products commonly falls around 31.5–34.0 g/L, depending on the manufacturer formula and agar content.
Comparative Medium Guide
| Medium | Primary Readout | Best Use | Key Strength | Limitation |
|---|---|---|---|---|
| Lysine Iron Agar (AS-1279) | Lysine decarb / deam / H₂S | Enteric identification panel | Highly informative biochemical differentiation | Usually not used as sole identification test |
| Triple Sugar Iron Agar (TSI) | Sugar fermentation / gas / H₂S | General enteric differentiation | Classic broad screening test | Does not assess lysine reactions |
| Urea Agar / Broth | Urease | Proteus / urease-positive organisms | Fast strong urease readout | Narrower biochemical scope |
| Simmons Citrate Agar | Citrate utilization | Supplementary enteric ID | Simple and widely recognized | Not a lysine/H₂S medium |
Interpretation Highlights
Typical positive patterns
- Purple butt may indicate lysine decarboxylation after glucose use
- Blackening indicates H₂S production
- Useful for recognizing many Salmonella-like reactions
Method considerations
- Interpret in conjunction with TSI, urea, citrate, motility, and serology as needed
- Use correct slant/deep preparation for valid reactions
- Read within validated SOP time window
Technical Specifications
| Catalogue Number | AS-1279 |
| Product Name | Lysine Iron Agar (LIA) |
| Format | Dehydrated medium |
| Use Format | Slant with butt / deep |
| Reconstitution | Approx. 31.5–34.0 g/L |
| Final pH | 6.7–6.8 ± 0.2 |
| Sterilization | 121°C for 15 min |
| HS Code | 3821.00.00 |
| Shelf Life | 3 years |
Common Organism Use Cases
- ✓ Salmonella spp.
- ✓ Proteus spp.
- ✓ Providencia spp.
- ✓ Citrobacter spp.
- ✓ Other enteric Gram-negative bacilli
Preparation & Handling
Suspend the dehydrated medium in purified water, heat to dissolve fully, dispense into tubes, sterilize, and cool in a slanted position to produce a deep butt with slant. Use according to validated internal SOPs and interpretation criteria.
Suggested Cross-Links
- Triple Sugar Iron Agar (TSI)
- Simmons Citrate Agar
- Urea Agar / Urea Broth
- Motility Test Medium
- XLD Agar / HE Agar / SS Agar
AuSaMicS Compliance & Supply Notes
Supplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer and compliance requirements.
For laboratory and research use only. Not for food, household, therapeutic, or pharmaceutical applications.
AuSaMicS Pty Ltd
31 Longview CT, Thomastown, VIC 3074, Australia
ABN 56676640467
www.ausamics.com.au
support@ausamics.com
+61 412 520 598