MRS Agar (De Man, Rogosa & Sharpe)

MRS Agar | De Man, Rogosa & Sharpe Medium

Selective Medium for Lactic Acid Bacteria
Cat No.: AS-1295

Description

MRS Agar (de Man, Rogosa and Sharpe Agar) is a selective, nutrient-rich microbiological culture medium specifically formulated for the isolation, enumeration, and cultivation of Lactobacillus species and other lactic acid bacteria (LAB).

Its optimized formulation of peptones, dextrose, yeast extract, and buffering agents supports strong LAB growth while suppressing competing microorganisms, making it a gold-standard medium in food, dairy, probiotic, and fermentation microbiology.

Product Details

• Catalog Number: AS-1295

• Format: Dehydrated powder

• Application: Microbiological culture medium

• Appearance: Beige to light yellow powder

• Storage: Store tightly sealed in a cool, dry place (≤30 °C)

Applications

• Cultivation and enumeration of Lactobacillus spp.

• Growth of other lactic acid bacteria (LAB)

• Probiotic research & quality control

• Food & dairy microbiology (yoghurt, cheese, fermented foods)

• Fermentation studies in academic and industrial laboratories

Key Features & Benefits

• High-nutrient formulation optimized for LAB growth

• Supports fastidious Lactobacillus strains

• Selective properties reduce background flora

• Consistent, reproducible batch performance

• Easy preparation – dissolve, sterilize, and pour

• Certificate of Analysis (CoA) available on request

Preparation (Typical)

1. Suspend the recommended amount of MRS Agar powder in purified water

2. Heat with agitation until completely dissolved

3. Sterilize by autoclaving at 121 °C for 15 minutes

4. Cool to 45–50 °C and pour into sterile Petri dishes

(Refer to internal SOPs or standard methods for exact concentrations.)

Safety & Intended Use

• For laboratory use only

• Not for human or animal consumption

• Refer to the MSDS/SDS for safe handling and disposal