Nutrient Broth | General Purpose Liquid Culture Medium
Nutrient Broth
General-Purpose Liquid Culture Medium
Catalog Number: AS-1309
Overview
Nutrient Broth is a non-selective, general-purpose liquid culture medium widely used in microbiology for the cultivation and maintenance of non-fastidious bacteria. Its balanced formulation supports reliable bacterial growth, making it a standard medium in teaching, research, and quality-control laboratories.
The combination of peptone and yeast extract supplies essential amino acids, peptides, vitamins, and growth factors, while sodium chloride maintains osmotic balance. Nutrient Broth is commonly used for enrichment cultures, routine growth, purity checks, and preparation of inocula prior to further biochemical, molecular, or immunological testing.
Product Details
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Catalog Number: AS-1309
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Form: Dehydrated powder
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Prepared medium: Clear to slightly opalescent broth after sterilisation
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HS Tariff Code: 3821.00 — prepared culture media for development of microorganisms
Typical Composition (per liter)
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Peptone: 5.0 g
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Yeast Extract: 3.0 g
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Sodium Chloride: 5.0 g
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Final pH: 7.2 ± 0.2 at 25 °C
Applications
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Cultivation of non-fastidious bacteria
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Enrichment of bacterial cultures
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Routine culture maintenance
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Purity testing prior to biochemical or immunological assays
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Preparation of inocula for further microbiological procedures
Key Features
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General-purpose, non-selective formulation
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Supports a wide range of bacterial species
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Reliable and reproducible growth performance
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Simple preparation and versatile laboratory use
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Suitable for teaching, research, and QC laboratories
Storage & Handling
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Dehydrated medium: Store at 15–30 °C in a dry, sealed container
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Prepared broth: Store at 2–8 °C
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Use prepared medium within recommended laboratory timeframes
Intended Use
For laboratory use only.
Not for medical, food, or veterinary applications.
Quality & Compliance
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Manufactured under controlled conditions
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Consistent batch-to-batch performance
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COA and SDS available upon request