Orange Serum Broth
Orange Serum Broth (OSB)
Selective Liquid Medium for Detection of Aciduric Spoilage Microorganisms
Product Identification
Chemical & Physical Information
Product Name: Orange Serum Broth (OSB)
Catalogue Number: AS-1313
Medium Type: Selective liquid culture medium
Form: Dehydrated powder
Product Description
Orange Serum Broth (OSB) is a selective liquid culture medium developed for the isolation, cultivation, and enumeration of aciduric microorganisms associated with the spoilage of fruit juices, soft drinks, wines, ciders, and other acidic food and beverage products.
The formulation supports the growth of acid-tolerant bacteria, yeasts, and moulds, including Lactobacillus, Leuconostoc, Acetobacter, and Gluconobacter species. The medium is widely used in beverage production facilities, wineries, breweries, juice processing plants, and contract microbiology laboratories for routine quality control testing.
Ausamics Orange Serum Broth is supplied as a high-quality dehydrated powder to ensure consistent selectivity, reproducible growth, and reliable recovery of spoilage organisms.
Principle of the Medium
Functional Components
Casein Enzymic Hydrolysate (Peptone): Provides nitrogen, peptides, and amino acids
Yeast Extract: Supplies vitamins and growth factors
Dextrose: Readily fermentable carbohydrate source
Dipotassium Hydrogen Phosphate: Buffering component
Orange Serum Solids: Provide natural organic acids and carbon sources characteristic of citrus products
The low final pH (approximately 5.5) suppresses most non-aciduric background flora while enabling robust growth of targeted spoilage microorganisms.
Typical Characteristics
Medium Properties
Appearance (dehydrated): Light beige to pale orange powder
Appearance (prepared): Clear to slightly opalescent amber broth
pH (prepared medium): 5.5 ± 0.2 at 25 °C
Selectivity: Aciduric microorganisms
Differential: No
Composition
Typical Formula (per litre)
Casein Enzymic Hydrolysate (Peptone): 10.0 g
Yeast Extract: 3.0 g
Dextrose: 4.0 g
Dipotassium Hydrogen Phosphate: 3.0 g
Orange Extract / Serum Solids: 5.0 g
Typical Applications
Beverage & Juice Microbiology
Detection of aciduric spoilage organisms in fruit juices, soft drinks, and concentrates
Quality control testing of acidic beverages and syrups
Fermentation & Production Monitoring
Enumeration of lactic acid bacteria and acetic acid bacteria
Monitoring of spoilage organisms in wine, cider, and beverage fermentation processes
Routine Quality Control
Microbiological testing in beverage production and processing facilities
Preparation Instructions
Medium Preparation
Suspend the required quantity of dehydrated medium in purified water
Heat with agitation until fully dissolved
Sterilise by autoclaving at 121 °C for 15 minutes
Cool to room temperature before use
Storage & Stability
Storage Conditions
Dehydrated medium: Store tightly sealed at 4–8 °C in a cool, dry place
Protect from moisture and direct light
Shelf life (unopened): 36 months from date of manufacture
Intended Use
Regulatory Statement
For food, beverage, fermentation, and laboratory quality control testing only.
Not intended for medical, veterinary, or therapeutic use.
Quality & Supply Assurance
Manufacturing & Documentation
Manufactured under controlled conditions
Consistent batch-to-batch quality
Certificate of Analysis (COA) available upon request
Technical support provided by Ausamics Life Science
Customs & Trade Information
Classification
HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms