PE-2 Broth (AS-1317) | Culture Media for Anaerobic Spore-formers

$39.00 AUD

AuSaMicS Life Science • Brewery Microbiology Media

PE-2 Broth

Selective Enrichment Broth for Beer Spoilage Bacteria

Highly selective liquid enrichment medium developed for detection of beer-spoilage pediococci and hop-resistant lactobacilli in brewery samples, pitching yeast, and finished beer. PE-2 Broth is widely used in brewery quality control programmes and is recognized in EBC and MEBAK workflows for forced spoilage detection.

AS-1317 🍺 Brewery QC Medium ✓ Selective Enrichment 🔬 Beer Spoilage Detection

Target: Pediococcus & hop-resistant Lactobacillus Reference Use: EBC / MEBAK brewery methods Readout: Turbidity, sediment, ropiness

Brewery Microbiology Specialist

Beer-Based Selection - Realistic brewery growth conditions

Yeast Suppression - Cycloheximine control

Spoilage Focused - Selects true beer spoilers

Routine Brewery QC - Pitching yeast to finished beer

Reference Method Style - Legacy brewery microbiology workflows

Forced Spoilage Enrichment

Designed for low-level brewery contaminants

🧪 Complete Formulation & Selectivity Profile

📋 Standard Brewery Formulation (per Liter)

Ingredient	Concentration	Primary Function	Contribution
Finished Beer / Unfiltered Lager	1000 mL	Natural selective base	Simulates real brewery growth conditions
Actidione (Cycloheximine)	10 mg/L	Yeast inhibitor	Suppresses culture yeast and wild yeasts

Final pH: As beer, typically 4.0–4.4. No adjustment required.

📋 Alternative Laboratory Version

Ingredient	Concentration	Purpose
Malt Extract Broth or Wort	1000 mL	Nutrient base
Ethanol	60–80 mL	Final alcohol level ~6–8% v/v
Cycloheximine	10 mg	Yeast inhibition
Hop Extract / Iso-α-Acids	20–30 EBC units	Selection for hop-resistant spoilers

⚗️ Functional Design

Real Beer Matrix
Uses beer or wort to mimic brewery spoilage conditions

Strong Selectivity
Ethanol, low pH, hops, and cycloheximine enrich true beer spoilers

Early Detection
Low-level contamination can be amplified before plating or confirmation

Typical positive reaction: Turbidity, sediment, and sometimes rope formation appearing after several days of incubation.

Complete Brewery Spoilage Detection Media Portfolio

Medium	Type	Target	Selectivity Basis	Best Application	Key Advantage
PE-2 Broth	Selective enrichment broth	Beer-spoilage pediococci & hop-resistant lactobacilli	Beer matrix + ethanol + hops + cycloheximine	Brewery contamination enrichment	Reference-style brewery spoilage detection
Universal Beer Agar	Solid detection medium	Beer spoilage bacteria	Beer-compatible nutrients and inhibitors	Isolation after enrichment	Colony visualization on plates
MRS Broth	LAB enrichment broth	Lactic acid bacteria	Nutrient selection rather than beer stress	General lactobacilli cultivation	Broad LAB support
Lactobacillus/Pediococcus Selective Media	Selective brewery medium	Specific brewery spoilers	Selective inhibitory system	Targeted brewery spoilage screening	Alternative selective workflow
MRS Agar	Isolation medium	Lactobacilli and related flora	Low pH / rich nutrient base	Subculture and confirmation	Routine LAB recovery

Strategic Medium Selection for Brewery Spoilage Monitoring

✅ PE-2 Broth Advantages

✓ Realistic Brewery Matrix: Beer or wort simulates actual spoilage conditions
✓ High Selectivity: Suppresses yeasts and non-spoiler organisms
✓ Low-Level Detection: Useful for forced enrichment of hidden contamination
✓ Reference Workflow: Recognized in brewery microbiology practice
✓ Broad Brewery QC Utility: Pitching yeast, finished beer, and process samples

⚠️ Method Considerations

▲ Longer Incubation: Positive tubes may require several days to develop
▲ Beer Quality Matters: Fresh, suitable beer matrix is important for consistency
▲ No Autoclaving After Cycloheximine: Supplement must be added aseptically
▲ Specialized Use: Intended for brewery microbiology rather than general lab testing

🎯 Best-Fit Applications

◆ Pitching Yeast QC: Detection of hidden spoilage flora
◆ Finished Beer Monitoring: Post-filtration contamination checks
◆ Process Hygiene Testing: Brewery sampling points and environmental control
◆ Forced Spoilage Studies: Enrichment of low-level contaminants

📊 Positive Reaction Guide

Observation	Interpretation	Typical Meaning	Notes
Turbidity	Positive growth	Beer-spoilage organism enrichment	Often visible from day 3 onward
Sediment	Positive growth	Possible pediococci or lactobacilli development	Check with subculture if needed
Ropiness	Strong presumptive positive	Spoilage-associated exopolysaccharide formation	Common with some pediococci

🔬 Quality Control & Performance Guidance

Typical Targets:
Pediococcus damnosus
Hop-resistant lactobacilli

Incubation:
25–28°C
5–14 days, anaerobic or microaerobic

Expected Positive:
Turbidity and/or sediment
Sometimes ropiness

Handling Note:
Do not autoclave after cycloheximine addition
Use aseptic supplement addition

Positioning: Specialized brewery microbiology enrichment broth for reference-style spoilage detection workflows in beer production and quality assurance programs.

📋 Technical Specifications

Catalogue Number	AS-1317
Medium Type	Selective enrichment broth
Target Organisms	Beer-spoilage pediococci and hop-resistant lactobacilli
Base Matrix	Beer, lager, wort, or malt extract broth
Cycloheximine	10 mg/L
Final pH	Typically 4.0–4.4
Incubation	25–28°C, 5–14 days
Atmosphere	Anaerobic or microaerobic / CO₂
Prepared Medium Storage	2–8°C, protected from light
Recommended Use Period	Within 1 month

🧫 Primary Applications

✓ Detection of low-level brewery spoilage contamination
✓ Monitoring of pitching yeast, filtered beer, and process samples
✓ Brewery hygiene and contamination trend studies
✓ Reference-style EBC / MEBAK enrichment workflows

⚗️ Preparation & Use Protocol

1. Use fresh filtered non-pasteurised beer, preferably lager, or prepare the alternative lab version.
2. Add filter-sterilized cycloheximine to a final concentration of 10 mg/L.
3. Dispense 10–50 mL into sterile screw-cap tubes or bottles.
4. Inoculate with sample or pitching yeast slurry.
5. Incubate under anaerobic or microaerobic conditions and inspect daily from day 3 onward.

📦 Storage & Safety

Storage: Store prepared PE-2 Broth at 2–8°C, protected from light.

Use period: Use within 1 month for optimal selectivity and performance.

Important note: Do not autoclave after adding cycloheximine.

Notice: For brewery microbiology use only.

AuSaMicS Life Science: Professional microbiology media for brewery quality control, industrial microbiology, research, and specialized contamination detection workflows. Product information is provided for laboratory and technical evaluation purposes only.