Phenol Red Agar Base
$35.00 AUD
AuSaMicS Life Science • Biochemical Identification Media
Phenol Red Agar Base
Carbohydrate Fermentation Agar Base
Standard solid base for preparing carbohydrate, polyalcohol, and glycoside fermentation agars. Widely used in conventional biochemical identification workflows for Enterobacteriaceae, non-fermenters, Gram-positive cocci, and other clinically or industrially relevant organisms.
AS-1325
🧪 Biochemical Base Medium
✓ Australian Stock
🔬 Fermentation Testing
Applications: Sugar and polyol fermentation studies
Indicator: Phenol Red
Format: Slants, deeps, or Durham tubes
Biochemical Media Specialist
Clear Indicator Shift - Red to yellow acid reaction
Low Peptone Base - Helps reduce alkaline reversion
Flexible Formulation - Add multiple sugars or polyols
Gas Detection Ready - Durham tube compatible
Classic Identification Tool - Routine microbiology workflows
Modular Fermentation Base
One base for many carbohydrate tests
🧪 Complete Formulation & Indicator Profile
📋 Base Composition (per Liter)
Final pH: 7.4 ± 0.2 at 25°C
⚗️ Functional Design
Colour-Based Reading
Phenol red provides clear acid/alkaline interpretation
Phenol red provides clear acid/alkaline interpretation
Low Reversion Risk
Lower nutrient loading helps reduce alkaline masking
Lower nutrient loading helps reduce alkaline masking
Flexible Test System
Supports multiple sugars, polyols, and glycosides
Supports multiple sugars, polyols, and glycosides
Carbohydrate Addition: Add 5–10 g/L of the desired carbohydrate or polyol to prepare specific fermentation media such as glucose, lactose, sucrose, mannitol, dulcitol, salicin, adonitol, or inositol variants.
Complete Biochemical Identification Media Portfolio
Strategic Medium Selection for Fermentation Testing
✅ Phenol Red Agar Base Advantages
- ✓ Versatile Base: Supports many sugars and polyols
- ✓ Easy Interpretation: Clear acid-associated yellow shift
- ✓ Gas Detection Compatible: Durham tube option
- ✓ Broad Utility: Routine biochemical identification labs
- ✓ Customizable: User selects carbohydrate according to method
⚠️ Method Considerations
- ▲ Carbohydrate Required: Base alone is not the final test medium
- ▲ Heat-Labile Sugars: Some carbohydrates should be filter-sterilized
- ▲ Slow Reactions: Some organisms need prolonged incubation
- ▲ Not a Standalone ID System: Best used with complementary biochemical tests
🎯 Best-Fit Applications
- ◆ Enterobacteriaceae Testing: Sugar fermentation profiles
- ◆ Clinical ID Labs: Supplementary biochemical confirmation
- ◆ Food & Water Labs: Conventional microbial characterization
- ◆ Teaching & QC Labs: Demonstration of fermentation reactions
📊 Interpretation Guide
🔬 Quality Control & Performance Guidance
Typical Use:
Preparation of fermentation agars
Conventional biochemical identification
Preparation of fermentation agars
Conventional biochemical identification
Target Organisms:
Enterics, non-fermenters, Gram-positive cocci
Broad routine microbiology use
Enterics, non-fermenters, Gram-positive cocci
Broad routine microbiology use
Physical Properties:
pH 7.4 ± 0.2
Red to orange base medium
pH 7.4 ± 0.2
Red to orange base medium
Preparation:
31.025 g/L base before carbohydrate addition
Autoclave after dispensing
31.025 g/L base before carbohydrate addition
Autoclave after dispensing
Reference Positioning: Commonly used as a classical fermentation test base in food, pharmaceutical, clinical, and educational microbiology laboratories.
📋 Technical Specifications
| Catalogue Number | AS-1325 |
| Medium Type | Carbohydrate fermentation agar base |
| Base Weight | 31.025 g/L |
| Final pH | 7.4 ± 0.2 (at 25°C) |
| Appearance | Red to orange base medium |
| Carbohydrate Addition | 5–10 g/L desired sugar or polyol |
| Sterilization | 121°C, 15 minutes |
| Heat-Labile Sugars | Use filter sterilization or lower-temperature handling where appropriate |
| Prepared Tube Storage | 2–8°C, protected from light |
| Recommended Use Period | Within 3 months for prepared tubes |
🧫 Primary Applications
- ✓ Preparation of fermentation agars for Enterobacteriaceae and other groups
- ✓ IMViC extension and conventional identification workflows
- ✓ API-style supportive biochemical testing
- ✓ Optional Durham tube gas detection studies
⚗️ Preparation Protocol
- 1. Suspend 31.025 g base plus 5–10 g of the chosen carbohydrate in 1 L purified water.
- 2. Heat with agitation and boil for 1 minute.
- 3. Dispense into tubes as slants or deeps, with Durham tubes if gas detection is required.
- 4. Autoclave at 121°C for 15 minutes, or use a reduced-temperature approach for heat-labile sugars where appropriate.
- 5. Allow to solidify as slants or butts before inoculation.
📦 Incubation, Storage & Safety
Incubation: 35–37°C for 18–48 h; slow reactions may require extended observation.
Prepared tubes: Store at 2–8°C, protected from light, and use within 3 months.
Interpretation: Yellow = acid, gas = Durham tube displacement, red = alkaline or no change.
Notice: For laboratory identification use only.
AuSaMicS Life Science: Professional microbiology media for biochemical identification, quality control, industrial, food, pharmaceutical, and research laboratories. Product information is provided for laboratory and technical evaluation purposes.