Skip to product information
Request a Quote
Thermoacidurans Agar (TAA) | AS-1362 | Bacillus coagulans Isolation | AuSaMicS
$45.00 AUD
AuSaMicS Life Science • Selective Cultivation Medium for Bacillus coagulans
Thermoacidurans Agar (TAA)
Thermoacidurans Agar is used for isolation and cultivation of Bacillus coagulans, historically also referred to as Bacillus thermoacidurans, from food products. The medium supports acid-tolerant thermophilic sporeformers associated with flat-sour spoilage in canned and acid food products.
Cat No.: AS-1362
Food Spoilage AgarTAAAustralian Stock
Format: Dehydrated culture medium SKU: To be assigned Support: COA / technical support available
Application Summary
Professional laboratory use
Reliable workflow integration
Australian stock
Premium AuSaMicS product-page format
Overview & Applications
Thermoacidurans Agar is used for isolation and cultivation of Bacillus coagulans, historically also referred to as Bacillus thermoacidurans, from food products. The medium supports acid-tolerant thermophilic sporeformers associated with flat-sour spoilage in canned and acid food products.
Primary Applications
- Isolation of Bacillus coagulans from food products
- Flat-sour spoilage investigations in canned foods
- Food microbiology and quality control workflows
- Heat-shock and sporeformer recovery studies
Key Features
- Acidic pH supports target organism selection
- Useful for Bacillus coagulans / thermoacidurans recovery
- Applicable to canned food spoilage investigations
- Classical APHA-linked food microbiology medium
Typical Composition / Product Characteristics
| Component / Attribute | Amount / Value | Function | Practical Role |
|---|---|---|---|
| Proteose peptone | 5.0 g/L | Nitrogen source | Supports growth of target sporeformers |
| Yeast extract | 5.0 g/L | Vitamin source | Improves recovery |
| Dextrose | 5.0 g/L | Energy source | Supports growth and metabolism |
| Dipotassium phosphate | 4.0 g/L | Buffer | Maintains medium stability |
| Manganese sulfate | 0.2 g/L | Trace nutrient | Supports physiological requirements |
| Agar | 20.0 g/L | Solidifying agent | Forms plate medium |
| Final pH | 5.0 ± 0.2 | Acidic environment | Favors thermoacidurans / coagulans recovery |
Preparation, Incubation & Interpretation
Preparation
Reconstitute the dehydrated medium in purified water, heat to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes, cool to 45–50°C, and pour into plates.
Incubation / Use
Apply the laboratory's validated spoilage investigation conditions. Thermophilic, acid-tolerant sporeformers are commonly assessed under elevated temperature conditions appropriate to the method.
Interpretation
Bacillus coagulans / B. thermoacidurans typically form cream to white colonies. Final interpretation should consider heat-shock pretreatment, colony morphology, and downstream confirmation.
Comparative Media / Workflow Position
| Medium / Product | Primary Target | Principle | Typical Use | Key Advantage |
|---|---|---|---|---|
| Thermoacidurans Agar | Bacillus coagulans | Acidic selective cultivation | Flat-sour spoilage investigations | Supports thermoacidurans/coagulans recovery |
| Dextrose Tryptone Agar | General sporeformers | Nutrient agar | Food spoilage studies | Less selective background |
| Plate Count Agar | Aerobic bacteria | Enumeration medium | Routine counts | Broad general utility |
| Nutrient Agar | General bacteria | Simple nutrient base | Routine cultivation | Non-selective baseline medium |
Recommended Workflow
1. Heat-shock sample
Apply heat treatment where the method targets sporeformers.
Apply heat treatment where the method targets sporeformers.
2. Plate onto TAA
Pour or spread according to the SOP.
Pour or spread according to the SOP.
3. Incubate appropriately
Use the validated elevated-temperature spoilage protocol.
Use the validated elevated-temperature spoilage protocol.
4. Confirm target colonies
Assess colony morphology and perform confirmatory testing if needed.
Assess colony morphology and perform confirmatory testing if needed.
Strategic Use
Advantages
- Useful for Bacillus coagulans food spoilage investigations
- Acidic formulation helps target relevant organisms
- Applicable to canned and acid food QC
Considerations
- Not intended as a broad general-purpose medium
- Best suited to validated food spoilage workflows
- Incubation conditions are method-dependent
Selection Guide
- Choose for flat-sour spoilage and Bacillus coagulans studies
- Useful in canned food and acid food microbiology
- Appropriate when acidic selective cultivation is desired