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Tryptic Soy Agar with Lecithin and Polysorbate 20 (MCT Agar; Microbial Content Test Agar) - AS-1434
$87.00 AUD
Tryptic Soy Agar with Lecithin and Polysorbate 20
SCDLP20 Agar — Neutralising Solid Medium for Cosmetics & Pharmaceutical Microbiology | Cat. No. AS-1434
Cat. No. AS-1434 SCDLP20 Agar | MCT Agar pH 7.3 ± 0.2 ~46 g/L ISO 21149 | ISO 18415 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
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Full DocumentationCOA, TDS & SDS included🇦🇺
Australian StockNo import delays⚡
Same-Week DispatchMelbourne warehouse🔬
Technical SupportDirect from our teamOverview
Tryptic Soy Agar with Lecithin and Polysorbate 20 (AS-1434) is the solid plating counterpart of SCDLP20/TAT Broth. It is a TSB-based agar supplemented with Lecithin (0.7 g/L) and Polysorbate 20 (Tween 20, 5 mL/L) as neutralising agents. It is used for colony isolation, plate counting, and environmental surface monitoring in cosmetics and pharmaceutical QC workflows, particularly after SCDLP20 Broth pre-enrichment.
Mode of Action
Dual Neutralisation on Solid Medium → Colony Recovery & Enumeration
Soya Lecithin (0.7 g/L) neutralises cationic QACs, cetrimide, chlorhexidine, and polymyxin B. Polysorbate 20 (5 mL/L) neutralises phenolics, parabens, benzoic acid esters, and in combination with lecithin also ethanol. At these concentrations, both agents are effective without inhibiting colony formation. The TSB nutritional base (casein + soy peptone) provides the amino acids, vitamins, and growth factors for broad microbial recovery on the agar surface.
Soya Lecithin (0.7 g/L) neutralises cationic QACs, cetrimide, chlorhexidine, and polymyxin B. Polysorbate 20 (5 mL/L) neutralises phenolics, parabens, benzoic acid esters, and in combination with lecithin also ethanol. At these concentrations, both agents are effective without inhibiting colony formation. The TSB nutritional base (casein + soy peptone) provides the amino acids, vitamins, and growth factors for broad microbial recovery on the agar surface.
Applications
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Colony Isolation
After SCDLP20 Broth enrichment
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Plate Counts
ISO 21149 / USP <61> enumeration
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Environmental
Swab & contact plate monitoring
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Cosmetics QC
Total aerobic microbial count
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Pharma QC
Non-sterile product testing
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GMP Monitoring
Cleanroom & production areas
SCDLP20 Agar vs SCDLP20 Broth — Workflow Position
| Step | Medium | Purpose |
|---|---|---|
| 1. Pre-enrichment / sample dilution | SCDLP20 Broth (AS-1357) | Neutralise preservatives; recover sub-lethally injured organisms; disperse viscous samples |
| 2. Plating / enumeration | SCDLP20 Agar (AS-1434) | Colony isolation, plate count, morphology assessment |
| 3. Confirmation (optional) | Selective/differential media | Species identification of colonies of interest |
Cross-Reference / Equivalent Products
| Manufacturer | Product Name | Cat. No. | Notes |
|---|---|---|---|
| Merck/Sigma | TSA with Lecithin and Polysorbate 80 (NutriSelect®) | MS_DS1717EN | Polysorbate 80 variant — similar agar base |
| BD BBL | Trypticase Soy Agar with Lecithin and Polysorbate 80 | 221283 | Equivalent plating agar (Polysorbate 80) |
| Oxoid | Tryptone Soya Agar | CM0131 | Base only — no neutralisers |
AuSaMicS AS-1434 uses Polysorbate 20 consistent with the SCDLP20 Broth counterpart. Some commercial equivalents use Polysorbate 80 — verify with your validated method before substitution.
Related Products
Product Identification
| Product Name | Tryptic Soy Agar with Lecithin and Polysorbate 20 |
| Common Name | TSA with Lecithin and Polysorbate 20; MCT Agar; Microbial Content Test Agar |
| Catalogue Number | AS-1434 |
| Liquid Counterpart | SCDLP20 Broth / TAT Broth (AS-1352) |
| Dissolution | ~46 g/L (add Polysorbate 20 separately as 5 mL/L) |
| Final pH at 25°C | 7.3 ± 0.2 |
| Sterilisation | Autoclave 121°C for 15 minutes |
| Pour Temperature | 45–50°C |
| Prepared Agar Appearance | Light amber, firm gel; slight opalescence from lecithin is normal |
| Dehydrated Appearance | Light beige free-flowing powder |
Formula — Per Litre
✓ Verified formula based on TSA + Lecithin + Polysorbate 20 standard. Reference: Merck MS_DS1717EN (Tween 80 variant), BD BBL 221283. Lecithin 0.7 g/L and Polysorbate 20 at 5 mL/L are the validated concentrations for solid agar applications.
| Component | Amount / L | Role |
|---|---|---|
| Casein Peptone (Tryptone) | 15.0 g | Primary nitrogen — peptides, amino acids |
| Soy Peptone | 5.0 g | Supplementary nitrogen, B-vitamins, cystine |
| Sodium Chloride (NaCl) | 5.0 g | Osmotic balance |
| Soya Lecithin | 0.7 g | QAC neutraliser — cetrimide, chlorhexidine, benzalkonium chloride |
| Polysorbate 20 (Tween® 20) | 5.0 mL | Phenolic & paraben neutraliser; synergistic with lecithin |
| Agar | 15.0 g | Solidifying agent — colony isolation & enumeration |
| Total (approx.) | ~46 g/L | Final pH 7.3 ± 0.2 at 25°C |
Preparation Protocol
| 1 | Suspend approximately 41 g of dehydrated base in 950 mL distilled water. Heat with stirring until completely dissolved. |
| 2 | Add 5 mL Polysorbate 20 (Tween 20). Mix until homogeneous. Adjust to 1 litre final volume. |
| 3 | Adjust pH to 7.3 ± 0.2 if required. |
| 4 | Autoclave at 121°C for 15 minutes. Do not over-autoclave — excessive heat reduces agar gel strength. |
| 5 | Cool to 45–50°C in a water bath. Pour ~20 mL per 90 mm Petri dish on a level surface. Allow to solidify. |
| 6 | Dry plates at 37°C / 30 min (lids ajar). Store at 2–8°C in sealed bags. Discard plates showing contamination, desiccation, or cracking. |
⚠️ Slight opalescence of prepared plates is normal due to lecithin — does not affect performance. Do not incubate plates directly from 2–8°C storage; equilibrate to room temperature to prevent condensation on colonies.
Quality Control — Performance Organisms
| Organism (ATCC) | Inoculum (CFU) | Incubation | Recovery |
|---|---|---|---|
| Staphylococcus aureus (6538) | ≤100 | 30–35°C / 48h | Good — typical colonies |
| Pseudomonas aeruginosa (9027) | ≤100 | 30–35°C / 48h | Good |
| Escherichia coli (8739) | ≤100 | 30–35°C / 48h | Good |
| Candida albicans (10231) | ≤100 | 20–25°C / 5 days | Good |
| Aspergillus brasiliensis (16404) | ≤100 | 20–25°C / 5 days | Good |
Regulatory Standards
| ISO 21149:2017 | Cosmetics — Enumeration of aerobic mesophilic bacteria (plating medium) |
| ISO 18415:2017 | Cosmetics — Detection of specified and non-specified microorganisms |
| USP <61> / <62> | Microbial examination of non-sterile products |
| EP 2.6.12 | Microbiological examination — non-sterile products |
Storage
| Dehydrated Powder | 15–30°C, tightly sealed, away from moisture and light |
| Prepared Plates | 2–8°C in sealed bags; use within 3 months |
| Shelf Life | Refer to expiry date on label |
📄 Full SDS available: 16-section GHS SDS (Australian WHS Regulations 2023 / GHS 7th Edition) — support@ausamics.com.au
Section 1 — Identification
| Product Name | Tryptic Soy Agar with Lecithin and Polysorbate 20 |
| Catalogue No. | AS-1434 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr) |
| Phone | +61 412 520 598 |
| support@ausamics.com.au |
Section 2 — Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | NOT classified as a hazardous substance under Australian WHS Regulations 2023. |
| Signal Word | None required |
| Thermal Hazard | Molten agar (45–50°C) presents a thermal burn risk during plate pouring — use appropriate thermal protection. |
Sections 4–8 — First Aid & PPE
| Inhalation | Remove to fresh air. Seek advice if symptoms persist. |
| Skin Contact (molten agar) | Cool immediately under cold running water. Seek medical attention for burns. |
| Eye Contact | Irrigate with water ≥15 min. Seek medical attention if irritation persists. |
| Ingestion | Rinse mouth. Call Poisons Centre 13 11 26 if large amounts ingested. |
| PPE | Thermal gloves for handling hot molten agar. Nitrile gloves and lab coat for handling inoculated plates. |
| Biosafety | Autoclave all inoculated plates (121°C / 15 min) before disposal as biological waste. |
Sections 13–15 — Disposal & Regulatory
| Disposal | Autoclave all inoculated plates before disposal per institutional biosafety procedures and local EPA regulations. |
| Transport | Not classified as dangerous goods — ADG, IMDG, IATA |
| Regulatory | Australian WHS Regulations 2023 | GHS 7th Edition | Safe Work Australia COP 2021 |
| AICIS | All components listed on AIIC or exempt |
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Light beige free-flowing powder; homogeneous | Visual |
| Appearance (prepared agar) | Light amber, firm gel; slight opalescence from lecithin — normal | Visual after pouring |
| pH (prepared, 25°C) | 7.3 ± 0.2 | pH meter (calibrated) |
| Gel Strength | Firm, uniform gel; no syneresis on plates | Visual / tilt test |
| Moisture (powder) | ≤5.0% | Loss on drying |
| Neutralisation — QAC | Colony recovery in presence of 0.1% benzalkonium chloride | Challenge test per lot |
| Neutralisation — Paraben | Colony recovery in presence of 0.1% methylparaben | Challenge test per lot |
| Growth Promotion | Good recovery of all 5 QC organisms per ISO 11133 criteria | Plate inoculation test |
| Batch COA | Available for every production lot | On request or included |
Manufacturing & Documentation
Manufactured by AuSaMicS Pty Ltd
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Neutralisation efficacy tested per batch
✓ Full GHS-compliant documentation (COA, TDS, SDS)
✓ Australian stock — no import delays
✓ ISO 21149 / ISO 18415 compliant formulation
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ Neutralisation efficacy tested per batch
✓ Full GHS-compliant documentation (COA, TDS, SDS)
✓ Australian stock — no import delays
✓ ISO 21149 / ISO 18415 compliant formulation
⚠️ Intended Use: For laboratory, pharmaceutical QC, cosmetics microbiology, and environmental monitoring use only. Not for human consumption or direct therapeutic use.