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Violet Red Bile Glucose Agar (VRBGA) | AS-1376 | Enterobacteriaceae | AuSaMicS
  • Violet Red Bile Glucose Agar (VRBGA) | AS-1376 | Enterobacteriaceae | AuSaMicS
Violet Red Bile Glucose Agar (VRBGA / VRBD) (AS-1376)

Violet Red Bile Glucose Agar (VRBGA / VRBD) (AS-1376)

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AuSaMicS Life Science • Selective Enumeration Agar for Enterobacteriaceae

Violet Red Bile Glucose Agar (VRBGA / VRBD)

Violet Red Bile Glucose Agar is a selective medium for isolation and enumeration of Enterobacteriaceae. It is a modification of MacConkey-type media in which glucose replaces lactose, enabling improved overall recovery of enterobacteria rather than specifically differentiating lactose fermenters.

Cat No.: AS-1376

Enterobacteriaceae AgarVRBG / VRBDAustralian Stock
Format: Dehydrated culture medium Support: COA / technical support available

Application Summary

Professional laboratory use
Reliable workflow integration
Australian stock
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Overview & Applications

Violet Red Bile Glucose Agar is a selective medium for isolation and enumeration of Enterobacteriaceae. It is a modification of MacConkey-type media in which glucose replaces lactose, enabling improved overall recovery of enterobacteria rather than specifically differentiating lactose fermenters.
Primary Applications
  • Enumeration of Enterobacteriaceae in food, pharmaceuticals, and cosmetics
  • Routine hygiene monitoring and quality control
  • Post-enrichment plating for overall enterobacteria counts
  • Useful where broader Enterobacteriaceae recovery is required
Key Features
  • Glucose-based formulation for Enterobacteriaceae enumeration
  • Bile salts and crystal violet inhibit Gram-positive bacteria
  • Neutral red supports colony contrast and halo interpretation
  • Classical medium for food and hygiene testing

Typical Composition / Product Characteristics

Component / Attribute Amount / Value Function Practical Role
Peptone from gelatin 7.0 g/L Primary nutrient source Supports enterobacterial growth
Yeast extract 3.0 g/L Vitamin source Enhances recovery
Sodium chloride 5.0 g/L Osmotic balance Maintains suitable ionic strength
Bile salts 1.5 g/L Selective agent Suppresses many Gram-positive organisms
Glucose 10.0 g/L Fermentable carbohydrate Supports Enterobacteriaceae recovery
Neutral red 0.03 g/L Indicator Helps visualize colony halos
Crystal violet 0.002 g/L Selective dye Additional suppression of Gram-positive bacteria
Agar 13.0 g/L Solidifying agent Forms plate medium
Final pH 7.4 ± 0.2 Optimized condition Supports intended selectivity and readout

Preparation, Incubation & Interpretation

Preparation

Suspend 39.5 g in 1 L distilled water. Bring to the boil to fully dissolve. Sterilize by boiling for about 1 minute with frequent agitation. Do not autoclave. Avoid prolonged heating.

Incubation / Use

Typical incubation is around 30–37°C for about 18–24 hours depending on the validated method.

Interpretation

Enterobacteriaceae typically produce dark red to purple colonies, often with reddish halos caused by glucose fermentation. Colony count and morphology should be interpreted according to the applicable standard method.

Comparative Media / Workflow Position

Medium / Product Primary Target Principle Typical Use Key Advantage
VRBGA / VRBD Enterobacteriaceae Selective glucose agar Food and hygiene enumeration Broader enterobacteria recovery
VRBA Coliforms Selective lactose agar Coliform enumeration Focused on lactose-fermenting coliforms
MacConkey Agar Gram-negative enterics Bile/dye differential agar General enteric differentiation Widely used clinical medium
Endo / EMB Coliforms and enterics Differential indicator agar Water and food work Classic lactose-based differentiation

Recommended Workflow

1. Prepare sample
Homogenize or dilute according to the validated food or hygiene method.
2. Plate medium
Pour or surface-inoculate onto VRBG agar.
3. Incubate
Use the standard incubation time and temperature for the target protocol.
4. Count colonies
Enumerate typical Enterobacteriaceae colonies and confirm if required.

Strategic Use

Advantages

  • Classical and widely used Enterobacteriaceae enumeration medium
  • Good recovery of overall enterobacteria
  • Useful in food, pharmaceutical, and cosmetic QC

Considerations

  • Not designed to differentiate lactose-positive from lactose-negative enterics in the same way as VRBA
  • Do not autoclave
  • Interpretation should follow the method-specific colony criteria

Selection Guide

  • Choose for Enterobacteriaceae counts rather than strict coliform counts
  • Useful in routine food hygiene testing
  • Good option when broader enterobacteria recovery is needed

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