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2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
  • 2× YT Agar (Double-Strength Yeast Tryptone Agar)
2× YT Agar (Double-Strength Yeast Tryptone Agar)

2× YT Agar (Double-Strength Yeast Tryptone Agar)

$25.00
Weight

2× YT Agar (Double-Strength Yeast Tryptone Agar)

Rich Medium for High-Density Growth of E. coli and Recombinant Bacteria

Catalog Number: AS-1395

Overview

2× YT Agar (Double-Strength Yeast Tryptone Agar) is a nutrient-rich, non-selective solid culture medium designed to support rapid and high-density growth of bacteria, particularly Escherichia coli used in molecular biology and recombinant DNA workflows.

Compared to standard LB or YT media, 2× YT Agar contains higher concentrations of yeast extract and tryptone, providing an increased supply of peptides, amino acids, vitamins, and growth factors. This enhanced formulation results in larger colonies, faster growth, and improved plasmid yield when used in cloning, expression, and phage-related applications.

Ausamics 2× YT Agar is manufactured to ensure consistent nutrient composition, reliable agar strength, and reproducible performance in research laboratories.

Key Features & Benefits

  • Double-strength formulation for enhanced bacterial growth

  • Rich in tryptone and yeast extract

  • Supports high cell density and rapid colony formation

  • Ideal for recombinant E. coli and plasmid-bearing strains

  • Suitable for antibiotic-supplemented selection plates

Typical Applications

Molecular Biology & Genetics

  • Cultivation of E. coli for cloning and plasmid propagation

  • Growth of recombinant and expression strains

  • Preparation of antibiotic selection plates

Phage & Protein Expression Work

  • Bacteriophage propagation

  • High-density bacterial lawns

  • Protein expression studies

Research & Teaching Laboratories

  • Routine bacterial culture

  • Demonstration of rapid growth media

  • Microbial physiology studies

Principle of the Medium

2× YT Agar provides an abundant supply of peptides and vitamins through tryptone and yeast extract, enabling bacteria to grow rapidly without nutritional limitation. Agar serves as a stable solidifying agent, allowing for clear colony morphology and uniform surface growth.

Typical Composition (per litre)

  • Tryptone – 16.0 g

  • Yeast Extract – 10.0 g

  • Sodium Chloride – 5.0 g

  • Agar – 15.0 g

Final pH: 7.0 ± 0.2 at 25 °C

(Composition may be adjusted to match specific institutional or published protocols.)

Preparation

  1. Suspend 46 g of dehydrated medium in 1 L purified water

  2. Heat with agitation until completely dissolved

  3. Sterilize by autoclaving at 121 °C for 15 minutes

  4. Cool to 50–55 °C

  5. Add antibiotics if required, mix gently, and pour plates

Storage & Stability

  • Dehydrated medium: Store at 15–30 °C in a cool, dry place

  • Prepared plates: Store at 2–8 °C, protected from drying

Intended Use

For laboratory research use only.
Not for human, veterinary, food, feed, or pharmaceutical use.

Quality & Compliance

  • Suitable for molecular biology and microbiology research

  • Manufactured under controlled conditions for batch consistency

  • Performance comparable to leading international suppliers

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

Why Buy 2× YT Agar from Ausamics?

Ausamics is an Australian life-science supplier specialising in research-grade microbiological media, offering:

  • Reliable local supply

  • Consistent quality and documentation

  • Competitive pricing

  • Responsive technical support

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