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MUG (Fluorogenic) | ASE-903 | beta-D-Glucuronidase Substrate
$25.00 AUD
Documentation
Enzyme Substrates · Environmental Testing · Molecular Biology
MUG (Fluorogenic Substrate)
4-Methylumbelliferyl-β-D-glucuronide dihydrate · CAS 881005-91-0
GUS Reporter Substrate
E. coli Detection
Ex 365nm / Em 450nm
High Sensitivity
AuSaMicS
Laboratory Division
Cat No.
ASE-903
CAS No.
881005-91-0
Signal
Fluorescent Blue
HS Code
2938.90.90
Storage
-20°C, Dark
Description
MUG (4-Methylumbelliferyl-β-D-glucuronide dihydrate) is an exceptionally sensitive fluorogenic substrate used for the detection of β-glucuronidase (GUS) activity. It is the gold standard for rapid microbial testing and reporter gene assays where higher sensitivity is required than that offered by traditional visible chromogens.
Detection Principle
The enzyme β-glucuronidase cleaves the glycosidic bond of MUG to release the aglycone 4-methylumbelliferone (4-MU). While the intact substrate is non-fluorescent, the 4-MU product exhibits intense blue fluorescence when excited by long-wave UV light (approx. 365 nm), allowing for qualitative visual confirmation or precise quantitative measurement via fluorometry.
Primary Applications
Water Microbiology
Rapid detection and enumeration of E. coli and coliforms in environmental water samples and food testing.
GUS Reporter Assays
Widely used in plant molecular biology and microbiology as a reporter for gene expression (uidA gene).
Diagnostic Media
Incorporation into fluorogenic confirmation media for rapid screening of β-glucuronidase-positive isolates.
Chemical Identity
| Catalogue Number | ASE-903 |
| CAS Number | 881005-91-0 (Dihydrate) / 6160-80-1 (Anhydrous) |
| Target Enzyme | β-D-Glucuronidase (GUS / uidA) |
| Excitation Maximum | ~365 nm |
| Emission Maximum | ~450 nm |
Storage & Handling
● Storage: Store tightly closed at −20°C in a desiccated environment.
● Light Sensitivity: Highly sensitive to light. Store in amber vials or dark conditions.
● Safety: Use appropriate UV protection when observing fluorescence results.
Ordering Information
Available in research-scale packs (100 mg to 5 g) and custom industrial bulk. AuSaMicS supports OEM and custom formulation for diagnostic kit manufacturers.
AuSaMicS ensures consistent batch-to-batch performance for sensitive fluorometric assays. Full technical support and documentation accompany each order.
TDS
Technical Data Sheet including fluorometric assay protocols.
SDS
Safety Data Sheet available for laboratory risk assessment.
COA
Certificate of Analysis confirming purity and fluorescence background specs.
Safety Notice: For laboratory research and industrial use only. Not for human, animal, or therapeutic use. Standard lab safety protocols must be followed.
Ausamics · Enzyme Substrates & Molecular Reagents · Cat No: ASE-903