4-Methylumbelliferyl β-D-Glucopyranosiduronic Acid (MUG)

4-Methylumbelliferyl β-D-Glucopyranosiduronic Acid (MUG)

$20.00
Weight

4-Methylumbelliferyl β-D-Glucopyranosiduronic Acid (MUG)

Fluorogenic Substrate for β-Glucuronidase Detection of Escherichia coli

Catalog Number: ASC-1019
CAS Number: 881005-91-0
Molecular Formula: C₁₆H₁₆O₉ · 2H₂O
Molecular Weight: 388.32 g/mol

Overview

4-Methylumbelliferyl β-D-Glucopyranosiduronic Acid (MUG) is a fluorogenic substrate widely used for the detection of β-glucuronidase activity, a key enzymatic marker associated with most strains of Escherichia coli.

Upon enzymatic hydrolysis by β-glucuronidase, MUG releases 4-methylumbelliferone, a compound that exhibits strong blue fluorescence under ultraviolet (UV) light. This fluorescence provides a rapid, sensitive, and highly specific indication of E. coli presence, making MUG an essential reagent in microbiological testing, food safety, water analysis, and research laboratories.

Ausamics MUG is supplied as a high-quality powder with consistent performance for routine and research applications.

Key Features & Benefits

  • Fluorogenic substrate for β-glucuronidase detection

  • Produces bright blue fluorescence under UV light

  • Enables rapid and sensitive identification of E. coli

  • Widely used in food, water, and microbiology testing

  • Reliable performance for routine and research laboratories

Typical Applications

Microbiology & Diagnostics

  • Detection of Escherichia coli via β-glucuronidase activity

  • Differentiation of E. coli from other coliform bacteria

  • Fluorogenic confirmation in culture-based assays

Food & Beverage Testing

  • Monitoring E. coli contamination in food products

  • Quality-control testing in food production environments

Water & Environmental Testing

  • Detection of E. coli in drinking water and water treatment systems

  • Monitoring microbial contamination in environmental samples

Research & Teaching Laboratories

  • Enzyme activity studies

  • Demonstration of fluorogenic enzyme reactions

  • Microbial physiology and screening assays

Principle of the Substrate

β-Glucuronidase cleaves the glucuronide bond of MUG, releasing 4-methylumbelliferone, which fluoresces under UV illumination (typically at 365 nm). The appearance of fluorescence indicates active β-glucuronidase, providing a clear and rapid method for identifying E. coli.

Physical & Chemical Properties

  • Appearance: White to off-white crystalline powder

  • Solubility: Soluble in water and suitable solvents

  • Reaction: Non-fluorescent → blue fluorescence after hydrolysis

  • Light sensitivity: Protect from light

Storage & Stability

  • Store at 2–8 °C or as specified on the label

  • Protect from light and moisture

  • Keep container tightly closed

  • Use before the stated expiry date

Intended Use

For laboratory research use only.
Not for human or animal use.
Not for food, feed, or pharmaceutical administration.

Quality & Documentation

  • Supplied with Certificate of Analysis (COA)

  • SDS available upon request

  • Manufactured and handled under controlled conditions

Customs & Trade Information

HS / AHECC Code: 2932.99.00
Heterocyclic organic compounds

Why Buy MUG from Ausamics?

Ausamics is an Australian life-science supplier specialising in microbiological substrates, dyes, and reagents, offering:

  • Reliable local supply

  • Consistent batch quality

  • Competitive research pricing

  • Responsive technical support

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