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A1 Medium (ATCC Medium No. 1)
  • A1 Medium (ATCC Medium No. 1)
  • A1 Medium (ATCC Medium No. 1)
  • A1 Medium (ATCC Medium No. 1)
  • A1 Medium (ATCC Medium No. 1)
A1 Medium (ATCC Medium No. 1)

A1 Medium (ATCC Medium No. 1)

$45.00
Weight

A1 Medium (ATCC Medium No. 1)

Highly Enriched Broth for Recovery of Fastidious Microorganisms

Catalog Number: AS-1101

Overview

A1 Medium, also known as ATCC Medium No. 1, is a highly enriched, non-selective liquid culture medium formulated for the maximum recovery and luxuriant growth of fastidious and delicate microorganisms. It is particularly effective for specimens containing low bacterial counts or stressed, injured, or antibiotic-exposed cells.

This medium is widely used in clinical and research microbiology laboratories for the enrichment and primary recovery of demanding bacterial species, including Streptococcus, Neisseria, Haemophilus, and Brucella. Its complex formulation supplies essential amino acids, vitamins, sulphur compounds, and growth factors, ensuring reliable growth even under challenging conditions.

Ausamics A1 Medium is manufactured to deliver consistent performance and reproducibility, in line with ATCC and international reference protocols.

Key Features & Benefits

  • Extremely nutrient-rich formulation for maximum recovery

  • Supports fastidious, slow-growing, and injured organisms

  • Excellent for low-inoculum clinical specimens

  • Recognised ATCC reference medium

  • Reliable performance in enrichment and recovery workflows

Typical Applications

Clinical & Research Microbiology

  • Enrichment and isolation of fastidious pathogens from:

    • Blood

    • Cerebrospinal fluid (CSF)

    • Sterile body fluids

Target Organisms

  • Streptococcus pneumoniae

  • Neisseria meningitidis

  • Haemophilus influenzae

  • Brucella spp.

Specialised Uses

  • Blood culture enrichment (often supplemented with SPS or neutralisers)

  • Recovery of microorganisms following:

    • Antibiotic treatment

    • Environmental or chemical stress

Principle of the Medium

A1 Medium provides a rich blend of peptones, yeast extract, carbohydrates, and sulphur-containing compounds that support metabolic recovery and rapid multiplication of nutritionally demanding bacteria. The inclusion of reducing agents aids in the recovery of oxygen-sensitive or injured cells, making the medium especially effective for primary isolation.

Composition (per litre)

  • Trypticase Peptone (Pancreatic Digest of Casein) – 20.0 g

  • Yeast Extract – 5.0 g

  • Glucose – 5.0 g

  • Sodium Chloride (NaCl) – 5.0 g

  • L-Cystine – 0.2 g

  • Sodium Sulphite (Na₂SO₃) – 0.1 g

  • Thiouracil – 0.01 g

  • Phenol Red (optional indicator) – 0.01 g

Final pH: 7.2 ± 0.2 at 25 °C

Preparation

  1. Suspend 35.31 g of dehydrated medium in 1 L purified water

  2. Mix thoroughly and warm gently until fully dissolved

  3. Dispense into tubes or bottles as required

  4. Sterilize by autoclaving at 121 °C for 15 minutes

  5. Cool to room temperature before use

Storage & Stability

  • Dehydrated medium: Store below 30 °C in a cool, dry place, protected from light

  • Prepared broth: Store at 2–8 °C and use within 14 days

Intended Use

For laboratory and research use only.
Not for human or veterinary consumption.
Not for diagnostic procedures without appropriate validation.

Quality & Documentation

  • Manufactured under controlled conditions for batch consistency

  • Certificate of Analysis (COA) available

  • SDS available upon request

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

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