Skip to product information
Arginine Brilliant-Green Glucose Peptone Broth
  • Arginine Brilliant-Green Glucose Peptone Broth
  • Arginine Brilliant-Green Glucose Peptone Broth
Arginine Brilliant-Green Glucose Peptone Broth

Arginine Brilliant-Green Glucose Peptone Broth

$45.00
Weight

Arginine Brilliant-Green Glucose Peptone Broth (ABGP)

Selective Enrichment Broth for Group B Streptococcus (GBS)
Catalog Number: AS-1122

Overview

Arginine Brilliant-Green Glucose Peptone Broth (ABGP) is a selective enrichment medium originally developed by Schubert (1966) for the isolation of Streptococcus agalactiae (Group B Streptococcus, GBS) from mixed microbial populations. It has been used for enrichment from clinical specimens (vaginal and rectal swabs, neonatal samples) as well as raw milk and dairy products.

The selectivity of ABGP Broth is achieved through the combined action of arginine, brilliant-green dye, and nalidixic acid, which strongly inhibit Gram-negative bacteria, enterococci, staphylococci, and most non-GBS streptococci. Under these conditions, GBS grows luxuriantly and produces a characteristic colour change from purple to yellow, resulting from arginine deamination followed by glucose fermentation.

ABGP Broth has been recommended in several older European protocols (notably in Germany, Switzerland, and Austria) as an alternative or complementary enrichment medium to LIM or Todd–Hewitt broth.

Applications

  • Selective enrichment of Streptococcus agalactiae (Group B Streptococcus) from mixed clinical samples

  • Screening of pregnant women for GBS carriage (typically at 35–37 weeks gestation)

  • Detection of Group B Streptococcus in raw milk and dairy products

  • Use as an alternative or complement to LIM or Todd–Hewitt enrichment media

Key Features & Benefits

  • Highly selective enrichment broth for Group B Streptococcus

  • Brilliant-green dye and nalidixic acid suppress most competing flora

  • Arginine promotes strong GBS growth and characteristic metabolic response

  • Built-in colour indicator system (purple → yellow) for presumptive detection

  • Historically validated formulation still used in selected laboratories

Composition (per litre)

  • Peptone – 10.0 g

  • Glucose – 5.0 g

  • L-Arginine hydrochloride – 5.0 g

  • Sodium chloride – 5.0 g

  • Disodium hydrogen phosphate (Na₂HPO₄) – 2.0 g

  • Brilliant Green – 0.01 g (10 mg)

  • Nalidixic Acid – 0.015 g (15 mg)

  • Bromocresol Purple – 0.02 g

Final pH: 7.2 ± 0.2 at 25 °C

Preparation

  • Dissolve all components except nalidixic acid and bromocresol purple in 900 mL distilled water

  • Adjust pH to 7.2

  • Add bromocresol purple dissolved in a few drops of 0.1 N NaOH

  • Make up to 1 L with distilled water

  • Dispense 5–10 mL into tubes

  • Sterilise by autoclaving at 121 °C for 15 minutes

  • After cooling to below 60 °C, aseptically add filter-sterilised nalidixic acid (15 mg/L)

(Some commercial formulations may include heat-stable nalidixic acid derivatives.)

Incubation & Interpretation

  • Incubation: 35–37 °C for 18–24 hours (up to 48 hours if required)

  • Positive reaction: Medium turns yellow (presumptive GBS growth)

  • Negative reaction: Medium remains purple

Subculture & Confirmation

Presumptive positive cultures should be subcultured onto:

  • Columbia CNA blood agar

  • Granada medium

  • CHROMagar™ StrepB

Storage & Stability

  • Prepared broth (with nalidixic acid): Store at 2–8 °C, protected from light

  • Shelf life: Use within 4 weeks

Intended Use

For laboratory use only.
Not for diagnostic use without appropriate validation.

Quality & Compliance

  • Selective enrichment medium for Group B Streptococcus

  • Manufactured under controlled conditions for consistency

  • Suitable for research, screening, and method-development applications

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

CUSTOMERS ALSO BOUGHT