Skip to product information
Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
  • Alkaline Peptone Water (APW)
Alkaline Peptone Water (APW)

Alkaline Peptone Water (APW)

$25.00
Weight

Alkaline Peptone Water (APW)

Selective Enrichment Broth for Vibrio Species

Catalog Number: AS-1105

Overview

Alkaline Peptone Water (APW) is a selective enrichment broth formulated for the isolation and recovery of Vibrio species, particularly Vibrio cholerae and Vibrio parahaemolyticus, from clinical specimens, seafood, and environmental water samples.

The medium’s high alkaline pH (8.4–8.6), combined with peptone and sodium chloride, promotes rapid multiplication of Vibrio spp. while inhibiting most competing enteric bacteria, including Enterobacteriaceae. APW is internationally recognised as the primary enrichment medium recommended by WHO, CDC, FDA, and ISO 21872-1 prior to plating on selective agars such as TCBS.

Key Features & Benefits

  • Selective alkaline pH (8.4–8.6) optimised for Vibrio growth

  • Rapid enrichment with visible turbidity in 6–18 hours

  • Simple, cost-effective, and globally standardised formulation

  • Essential first-step enrichment before selective plating

  • Widely used in public health, food safety, and environmental microbiology

Typical Applications

Clinical & Public Health Microbiology

  • Primary enrichment of Vibrio cholerae from faecal specimens and rectal swabs

  • Cholera surveillance and outbreak investigations

Food & Seafood Microbiology

  • Detection of Vibrio spp. in shrimp, oysters, fish, and shellfish

  • Food-safety monitoring and regulatory testing

Environmental & Water Testing

  • Isolation of Vibrio spp. from coastal, estuarine, and drinking water sources

  • Environmental surveillance and epidemiological studies

Principle of the Medium

Vibrio species exhibit rapid growth under alkaline conditions and tolerate elevated salt concentrations. APW exploits these characteristics by combining a nutrient-rich peptone base with alkaline pH, suppressing competing intestinal flora while allowing Vibrio spp. to proliferate before selective plating.

Composition (per litre)

  • Peptone (Bacteriological Peptone) – 10.0 g

  • Sodium Chloride (NaCl) – 10.0 g

Final pH: 8.5 ± 0.2 at 25 °C
(Adjusted with 1 N NaOH if required)

Preparation

  1. Dissolve 10 g peptone and 10 g NaCl in 1 L distilled or deionised water

  2. Mix thoroughly until completely dissolved

  3. Adjust pH to 8.5 using 1 N NaOH if necessary

  4. Dispense into tubes or flasks (commonly 10 mL volumes)

  5. Sterilise by autoclaving at 121 °C for 15 minutes

  6. Cool to room temperature before use

Common Variants

  • Marine / estuarine Vibrio (e.g. V. parahaemolyticus):
    NaCl increased to 20–30 g/L

  • Less halophilic Vibrio (e.g. V. vulnificus):
    NaCl reduced to 5 g/L

Incubation

  • 35–37 °C for 6–18 hours (primary enrichment)

  • A second enrichment (subculture into fresh APW) is recommended for heavily contaminated samples

Storage & Shelf Life

  • Dehydrated medium: Store dry at room temperature, protected from moisture

  • Prepared broth: Store at 2–8 °C; stable for up to 3 months

Intended Use

For laboratory and research use only.
Not for diagnostic use without appropriate validation.
Not for food, therapeutic, or veterinary applications.

Quality & Documentation

  • Manufactured under controlled conditions for batch consistency

  • Certificate of Analysis (COA) available

  • Safety Data Sheet (SDS) available upon request

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

CUSTOMERS ALSO BOUGHT