Skip to product information
Antibiotic Medium No. 3
  • Antibiotic Medium No. 3
  • Antibiotic Medium No. 3
  • Antibiotic Medium No. 3
  • Antibiotic Medium No. 3
Antibiotic Medium No. 3

Antibiotic Medium No. 3

$45.00
Weight

Antibiotic Medium No. 3 (Turbidimetric Assay Broth)

Official Liquid Medium for Turbidimetric (Photometric) Antibiotic Assays
Catalog Number: AS-1110

Overview

Antibiotic Medium No. 3 is the official standardized liquid medium used for the cultivation of assay organisms and for the turbidimetric (photometric) microbiological assay of antibiotics. It is designated as “Medium C” in all major global regulatory and pharmacopeial standards, including FDA 21 CFR 436.102, USP <81>, European Pharmacopoeia 2.7.2, British Pharmacopoeia, Japanese Pharmacopoeia (JP 17), WHO, and ISO 20776-2.

The balanced nutrient composition supports rapid, uniform, and heavy growth of standard assay organisms such as Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, and Kocuria rhizophila. This ensures accurate, sensitive, and reproducible antibiotic potency determination using the tube-turbidimetric method.

Ausamics Antibiotic Medium No. 3 is manufactured to deliver minimal batch-to-batch variation, consistent growth kinetics, and reliable compendial performance for regulated pharmaceutical quality-control laboratories.

Applications

  • Turbidimetric (photometric) antibiotic potency assays

  • Preparation of dense inoculum suspensions for cylinder-plate assays

  • Growth and maintenance of test organisms for USP, EP, and JP antibiotic bioassays

  • Pharmaceutical quality-control laboratories using compendial methods

Key Features & Benefits

  • Internationally harmonised formulation (USP / EP / BP / JP / WHO / ISO)

  • Produces dense, uniform growth within 3–5 hours at 37 °C

  • Highly consistent growth response with low batch-to-batch variability

  • Recognised reference broth for regulatory turbidimetric antibiotic assays

  • Supports a wide range of standard assay organisms

Composition (per litre)

  • Beef Extract – 1.5 g

  • Yeast Extract – 3.0 g

  • Peptone (meat or casein) – 6.0 g

  • D-Glucose – 1.0 g

Final pH: 7.0 ± 0.1 at 25 °C (after sterilisation)

Preparation

  • Dissolve 11.5 g of dehydrated medium in 1 L purified water

  • Mix thoroughly until completely dissolved

  • Dispense into tubes or flasks as required

  • Autoclave at 121 °C for 15 minutes

  • Cool to room temperature before use

Storage & Stability

  • Dehydrated medium: Store below 30 °C in a cool, dry place

  • Prepared broth: Store at 2–8 °C

  • Shelf life (prepared broth): Use within 1 month

Standard Test Organisms (Examples)

  • Staphylococcus aureus ATCC 6538P

  • Bacillus subtilis ATCC 6633

  • Klebsiella pneumoniae subsp. pneumoniae ATCC 10031

  • Kocuria rhizophila ATCC 9341

Intended Use

For pharmaceutical quality-control and compendial antibiotic assay applications only.
Not for clinical diagnostic, food, or veterinary use.

Quality & Compliance

  • Official reference medium for turbidimetric antibiotic potency assays

  • Complies with USP <81>, EP 2.7.2, JP 17, WHO, FDA, and ISO requirements

  • Manufactured under controlled conditions for batch consistency

  • COA, SDS, and TDS available upon request

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

CUSTOMERS ALSO BOUGHT