Bile Salts Mixture
Bile Salts Mixture
Selective Agent for Enteric Bacteria Culture Media
Catalog Number: AS-1002
Overview
Bile Salts Mixture is a selective inhibitory agent widely used in microbiological culture media for the isolation and differentiation of enteric Gram-negative bacteria. Derived from purified bile acids, this mixture suppresses Gram-positive organisms while allowing the growth of bile-tolerant enteric pathogens such as Escherichia coli, Salmonella, Shigella, and Enterococcus (in appropriate formulations).
Bile Salts Mixture is a key component in many selective and differential media, including MacConkey Agar, Deoxycholate Citrate Agar, and various bile-based enrichment and transport media. It plays a critical role in food safety, water testing, clinical microbiology, and environmental monitoring.
Applications
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Selective inhibition of Gram-positive bacteria in culture media
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Component of MacConkey Agar, DCA, SS Agar, and related media
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Food, water, and environmental microbiology testing
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Clinical microbiology and enteric pathogen research
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Media formulation and custom microbiological media development
Key Features
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Strong inhibition of Gram-positive organisms
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Supports growth of bile-tolerant enteric bacteria
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Consistent performance in selective and differential media
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High purity and batch-to-batch reproducibility
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Suitable for routine, research, and regulatory microbiology
Principle of Action
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Bile salts disrupt cell membranes of Gram-positive bacteria
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Enteric Gram-negative bacteria exhibit bile resistance and survive
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Selectivity improves target organism recovery and colony differentiation
This selective pressure enhances accuracy in pathogen detection and enumeration.
Typical Chemical Characteristics
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Appearance: Pale yellow to yellow-brown powder
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Solubility: Partially soluble in water
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Odour: Characteristic
Storage & Stability
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Store in a cool, dry place below 30 °C
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Protect from moisture and direct light
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Reseal container tightly after use
Intended Use
For laboratory, research, food, water, and environmental microbiology use only.
Not intended for therapeutic, diagnostic, or human consumption.
Quality & Compliance
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Manufactured under controlled conditions
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Suitable for ISO, FDA-BAM, and APHA-aligned microbiology workflows
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Certificate of Analysis (COA) available
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Safety Data Sheet (SDS) available upon request