Brilliant Green Agar (BGA)
$35.00 AUD
Brilliant Green Agar (BGA)
ISO 6579-1 Reference Medium for Salmonella Isolation
CAT No.: AS-1150
Overview
Brilliant Green Agar (BGA) is a highly selective solid culture medium designed for the isolation and differentiation of Salmonella spp. (excluding S. Typhi and S. Paratyphi) from food, feed, and environmental samples.
It is the classical reference plating medium used globally and remains the mandatory second selective agar in ISO 6579-1:2017, used in parallel with XLD or equivalent media. BGA is widely adopted across Europe, Asia, and international food safety laboratories, particularly in poultry, egg, and meat testing.
Ausamics Brilliant Green Agar is manufactured to meet the performance expectations of ISO, DIN, and international reference methods, comparable to leading brands such as Difco™, Merck™, and Oxoid™.
Applications
-
Direct plating after selective enrichment
(Rappaport-Vassiliadis, Selenite Cystine, MKTTn) -
Official second selective agar in:
-
ISO 6579-1:2017 (Annex D)
-
DIN 10160
-
Multiple national and regional Salmonella testing methods
-
-
Routine Salmonella screening in:
-
Poultry & egg products
-
Meat & meat products
-
Food processing environments
-
Environmental monitoring samples
-
Key Features & Selectivity
-
Very strong inhibition of competing flora
(E. coli, Proteus, Pseudomonas) via:-
Brilliant Green dye
-
Phenol Red indicator system
-
-
Clear and characteristic Salmonella colony morphology
-
Reliable differentiation based on lactose/sucrose fermentation
-
Performance aligned with Edel–Kampelmacher formulation
Colony Characteristics
(18–24 hours at 35–37 °C)
| Organism | Appearance |
|---|---|
| Salmonella spp. (non-typhi) | Pink-red to red colonies, 1.5–4.0 mm, smooth; H₂S-positive strains may show greenish-black centres |
| E. coli / Coliforms | Strongly inhibited or very small yellowish colonies |
| Proteus / Pseudomonas | Inhibited or tiny pale colonies |
Composition (per litre)
(Modified Edel–Kampelmacher formulation)
| Component | Quantity |
|---|---|
| Yeast Extract | 3.0 g |
| Peptone / Proteose Peptone | 10.0 g |
| Lactose | 10.0 g |
| Sucrose | 10.0 g |
| Sodium Chloride | 5.0 g |
| Phenol Red | 0.08 g |
| Brilliant Green | 0.0125 g (12.5 mg) |
| Agar | 12.0–15.0 g |
Final pH: 6.9 ± 0.2 at 25 °C
Preparation Instructions
-
Suspend 50.0 g of dehydrated medium in 1 L purified water
-
Heat with frequent agitation and boil for 1 minute
-
DO NOT AUTOCLAVE
(Brilliant Green is heat-labile and excessive heat reduces selectivity) -
Cool to 45–50 °C, mix thoroughly
-
Pour plates and allow to solidify
Storage & Stability
-
Dehydrated medium:
Store in a cool, dry place below 30 °C, protected from light -
Prepared plates:
Store at 2–8 °C, protected from light
Use within 7 days
Quality & Compliance
-
Conforms to ISO 6579-1:2017 performance requirements
-
Manufactured under controlled conditions with batch traceability
-
Suitable for routine, regulatory, and accredited food microbiology laboratories
HS Tariff Code
-
HS Code: 3822.00
(Prepared diagnostic or laboratory reagents)
Comparison with Leading Brands
| Feature | Ausamics BGA | Difco™ | Oxoid™ | HiMedia™ |
|---|---|---|---|---|
| ISO 6579-1 compliant | ✅ | ✅ | ✅ | ✅ |
| Strong inhibition of coliforms | ✅ | ✅ | ✅ | ✅ |
| Edel-Kampelmacher formulation | ✅ | ✅ | ✅ | ✅ |
| Clear Salmonella morphology | ✅ | ✅ | ✅ | ✅ |
| Cost-effective supply | ⭐⭐⭐⭐ | ⭐⭐ | ⭐⭐ | ⭐⭐⭐ |
| Local Australian availability | ✅ | ❌ | ❌ | ❌ |
Why Buy from Ausamics?
-
Australian-based supplier with fast local dispatch
-
Transparent documentation & technical support
-
Competitive pricing without compromising reference performance
-
Trusted by food, environmental, and microbiology laboratories
Intended Use & Disclaimer
For food and environmental microbiology use only.
Not intended for diagnostic or clinical use in humans or animals.
Official References
-
ISO 6579-1:2017, Annex D
-
Edel, W. & Kampelmacher, E.H. (1969)