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Bromocresol Purple Azide Broth | Selective Medium for Enterococci | AS‑1154
$60.00 AUD
AuSaMicS Life Science • Culture Media
Bromocresol Purple Azide Broth
Selective broth for enterococci and related faecal streptococci screening
Selective differential broth formulated for the detection, enrichment, and presumptive differentiation of enterococci and related faecal streptococci. The medium combines sodium azide to suppress much of the accompanying Gram-negative flora with bromocresol purple as a pH indicator to reveal carbohydrate fermentation through visible color change.
Cat. No: AS-1154
Selective Differential Broth
✓ Australian Stock
🧫 Enterococci Medium
🚀 Fast Dispatch: Australian supply
🧪 Microbiological Grade: Routine QC and lab use
📋 Documentation: COA + SDS available
🏆 Selective Enterococci Screening
🛡️ Sodium Azide - suppresses many Gram-negative bacteria
🟣 Bromocresol Purple - visible pH indicator response
🔬 Presumptive Differentiation - fermentation-based reading
💧 Water & Hygiene Testing - useful in routine labs
📞 Technical Support - workflow matching assistance
Indicator-Based Broth Reading
Designed for rapid visual interpretation after incubation
🔬 Product Overview
Bromocresol Purple Azide Broth is a selective enrichment and screening broth commonly used for the presumptive detection of enterococci, faecal streptococci, and related azide-tolerant Gram-positive cocci. The medium is designed to encourage growth of the target group while restricting many competing Gram-negative organisms.
Fermentation of the carbohydrate source lowers the pH of the medium, producing a color response in the bromocresol purple indicator. This makes the broth useful in routine water testing, food microbiology, environmental monitoring, and hygiene control workflows where enterococci are used as indicator organisms.
⚗️ Principle of the Medium
Sample inoculation
↓
Growth in azide-containing selective broth
↓
Target organisms ferment carbohydrate source
↓
Acid production shifts bromocresol purple indicator color
↓
Growth in azide-containing selective broth
↓
Target organisms ferment carbohydrate source
↓
Acid production shifts bromocresol purple indicator color
Sodium azide inhibits many respiratory Gram-negative bacteria, improving selectivity for enterococci and related organisms. The pH indicator bromocresol purple provides a simple visual readout of fermentation activity after incubation.
🧬 Key Applications
💧 Water Microbiology
Useful in workflows involving the detection of enterococci or faecal streptococci as indicators of sanitary quality and faecal contamination.
🍽️ Food & Beverage Testing
Applicable in food microbiology and hygiene investigations where enterococci are relevant indicators of process or handling contamination.
🏭 Environmental & Surface Monitoring
Can be used in broader environmental monitoring programs to enrich and screen for azide-tolerant Gram-positive indicator flora.
🔍 Presumptive Screening Step
Functions well as a presumptive enrichment broth prior to plating on confirmatory agar media or performing additional biochemical identification tests.
✨ Key Features
- Selective azide-containing formulation for enterococci-focused recovery
- Bromocresol purple indicator for visible pH-based differentiation
- Suitable for routine water and hygiene laboratories
- Useful as an enrichment or presumptive test medium
- Supports downstream confirmatory testing workflows
- Available with COA and SDS support
🧪 Typical Composition (g/L)
Exact formulation may vary slightly according to manufacturing standard and validated performance requirements.
📊 Related Enterococci Media Comparison
💡 Preparation & Use Guidance
Use Case:
For selective enrichment and presumptive screening of enterococci-type flora
For selective enrichment and presumptive screening of enterococci-type flora
Reading:
Interpret growth and indicator color shift after incubation
Interpret growth and indicator color shift after incubation
Next Step:
Subculture positive broths to confirmatory agar or biochemical tests
Subculture positive broths to confirmatory agar or biochemical tests
Storage:
Store dehydrated medium tightly closed in a cool, dry place
Store dehydrated medium tightly closed in a cool, dry place
📋 Technical Specifications
| Product Name | Bromocresol Purple Azide Broth |
| Catalog Number | AS-1154 |
| Format | Dehydrated powder |
| Medium Type | Selective differential broth |
| Primary Function | Enterococci / faecal streptococci screening |
| Selective Agent | Sodium azide |
| Indicator | Bromocresol purple |
| Appearance | Cream to pale beige powder |
| Prepared Medium | Purple broth |
| Storage | Keep tightly closed in a dry place, protected from light and moisture |
🎯 Typical Organism Scope
- ✓ Enterococcus spp.
- ✓ Faecal streptococci
- ✓ Azide-tolerant Gram-positive cocci
- ✓ Indicator organisms in sanitary testing
✅ Quality & Documentation
- ✓ Microbiological grade raw materials
- ✓ Batch QC documentation
- ✓ COA available
- ✓ SDS support
📄 Available Documents
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)
🧫 Related AuSaMicS Media
Need a complete enterococci detection workflow?
AuSaMicS can support selective broths, confirmatory agars, and technical documentation for water, food, and sanitation microbiology workflows.
For laboratory and research use only. Use according to validated laboratory methods and internal quality procedures.