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BROLAC Agar | Bromothymol‑Blue Lactose Agar for Enteric Differentiation | AS‑1153
$55.00 AUD
AuSaMicS Life Science • Culture Media
BROLAC Agar
Bromothymol-blue Lactose Agar for lactose fermentation differentiation
Differential solid medium formulated for the detection and differentiation of lactose-fermenting and non-lactose-fermenting microorganisms. BROLAC Agar uses lactose as a fermentable carbohydrate and bromothymol blue as the pH indicator, enabling clear visual distinction based on acid production and colony-associated color change.
Cat. No: AS-1153 Differential Agar ✓ Australian Stock 🧫 Lactose Indicator Medium
🚀 Fast Dispatch: Australian supply 🧪 Microbiological Grade: Routine laboratory use 📋 Documentation: COA + SDS available
🏆 Clear Lactose Differentiation
🟡 Lactose Fermentation - acid reaction visible
🔵 Bromothymol Blue - pH-based color contrast
🔬 Differential Use - colony reaction screening
🍽️ Food & Water Labs - routine microbiology fit
📞 Technical Support - method alignment assistance
Indicator-Based Reading
Designed for rapid visual interpretation of lactose fermentation behavior
🔬 Product Overview
BROLAC Agar is a differential microbiological medium developed for the cultivation and differentiation of organisms based on their ability to ferment lactose. The medium incorporates bromothymol blue as a pH indicator, allowing acid-producing organisms to be distinguished from non-fermenters through visible color change in the colony and surrounding medium.
It is suitable for routine microbiology laboratories performing screening, isolation support, and preliminary differentiation of enteric and other lactose-utilizing organisms from food, water, environmental, and general laboratory samples.
⚗️ Principle of the Medium
Lactose in medium
↓
Lactose-fermenting organism
↓
Acid production lowers local pH
↓
Bromothymol blue shifts color, aiding colony differentiation
↓
Lactose-fermenting organism
↓
Acid production lowers local pH
↓
Bromothymol blue shifts color, aiding colony differentiation
Organisms capable of lactose fermentation produce acid, causing a change in the pH indicator response. Non-lactose fermenters generally retain the original medium appearance or produce distinctly different colony reactions, making the medium useful for rapid presumptive differentiation.
🧬 Key Applications
🦠 Enteric Differentiation
Useful for separating lactose-fermenting organisms from non-fermenters in routine enteric screening workflows.
🍽️ Food Microbiology
Applicable in food and beverage laboratories for preliminary differentiation of lactose-utilizing bacteria from samples and production environments.
💧 Water & Environmental Testing
Can be used as part of broader indicator-organism and hygiene-monitoring workflows where lactose fermentation is a useful screening criterion.
🔍 Preliminary Identification Support
Supports presumptive differentiation before biochemical confirmation or subculture onto more selective media.
✨ Key Features
- Lactose-based differentiation for rapid visual screening
- Bromothymol blue indicator enhances reaction visibility
- Suitable for routine microbiology laboratories
- Supports colony characterization before confirmation
- Useful in food, water, and general microbiology workflows
- Available with COA and SDS support
🧪 Typical Composition (g/L)
Exact formulation may vary slightly by manufacturing standard and validated performance requirements.
📊 Related Differential Media Comparison
💡 Preparation & Use Guidance
Use Case:
For differentiation based on lactose fermentation reactions
For differentiation based on lactose fermentation reactions
Reading:
Observe colony color and surrounding medium response after incubation
Observe colony color and surrounding medium response after incubation
Next Step:
Confirm presumptive results with biochemical or selective follow-up tests
Confirm presumptive results with biochemical or selective follow-up tests
Storage:
Store dehydrated medium tightly closed in a cool, dry place
Store dehydrated medium tightly closed in a cool, dry place
📋 Technical Specifications
| Product Name | BROLAC Agar |
| Full Name | Bromothymol-blue Lactose Agar |
| Catalog Number | AS-1153 |
| Format | Dehydrated powder |
| Medium Type | Differential agar |
| Primary Function | Lactose fermentation differentiation |
| Appearance | Light beige to greenish powder |
| Prepared Medium | Green to blue-green agar |
| Storage | Keep tightly closed in a dry place, protected from moisture and light |
| Application | Food, water, environmental, and routine microbiology |
🎯 Typical Organism Scope
- ✓ Lactose-fermenting enterics
- ✓ Non-lactose-fermenting screening isolates
- ✓ Routine mixed culture differentiation
- ✓ Indicator organism workflows
✅ Quality & Documentation
- ✓ Microbiological grade raw materials
- ✓ Batch QC documentation
- ✓ COA available
- ✓ SDS support
📄 Available Documents
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)
🧫 Related AuSaMicS Media
Need a complete lactose-differentiation workflow?
AuSaMicS can support plating media, enrichment broths, and technical documentation for food, water, and routine microbiology workflows.
For laboratory and research use only. Use according to validated laboratory methods and your internal quality procedures.