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Buffered Listeria Enrichment Broth Base | Selective Enrichment for Listeria spp. | AS‑1157

$45.00 AUD
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AuSaMicS Life Science • Culture Media

Buffered Listeria Enrichment Broth Base

Selective enrichment broth base for Listeria recovery from food, environmental, and hygiene samples

Buffered Listeria Enrichment Broth Base is a high-performance pre-enrichment and selective enrichment medium base developed for the recovery of Listeria spp., including Listeria monocytogenes, from food products, raw materials, environmental swabs, and production hygiene samples. Its buffered nutrient system helps support injured or stressed cells, while the final supplemented formulation improves selectivity against competing flora.

Cat. No: AS-1157 Selective Enrichment Broth Base ✓ Australian Stock 🧫 Listeria Workflow
🚀 Fast Dispatch: Australian supply 🧪 Microbiological Grade: Food microbiology ready 📋 Documentation: COA + SDS available

🏆 Listeria Recovery Focus

🛡️ Buffered System - helps protect stressed cells
🧫 Selective Workflow Base - designed for supplement addition
🍽️ Food & Environmental Use - routine Listeria screening
🔬 Recovery-Oriented - suitable for low-level contamination work
📞 Technical Support - method guidance available
Supplement-Ready Base
Used with selective supplements according to the validated Listeria method

🔬 Product Overview

Buffered Listeria Enrichment Broth Base is a nutrient-rich, buffered medium base developed for the selective enrichment of Listeria species from samples that may contain competing background flora or sublethally injured target cells. The formulation supports recovery before subsequent isolation on selective Listeria agars.

It is commonly used in food microbiology, dairy testing, ready-to-eat product monitoring, raw material screening, and environmental hygiene programs. When prepared with the appropriate selective supplements, it becomes a practical enrichment step in validated Listeria detection workflows.

⚗️ Principle of the Medium

Sample inoculation

Buffered nutrient enrichment

Recovery and multiplication of Listeria spp.

Subculture to selective Listeria agar after enrichment

The buffered composition helps reduce the effects of sample-associated stress while providing nutrients for target recovery. Selective agents, added according to the intended method, improve suppression of competing organisms and enhance the recovery of Listeria from complex sample matrices.

🧬 Key Applications

🍽️ Food Microbiology

Suitable for detection workflows involving foods, dairy, ready-to-eat products, raw ingredients, and other materials where Listeria is a critical target organism.

🏭 Environmental Monitoring

Useful for swabs, drains, surfaces, and food-production environment monitoring programs focused on Listeria control.

🧪 Recovery of Injured Cells

Buffered enrichment conditions help support stressed or sublethally damaged Listeria cells that may be difficult to recover directly on strongly selective plating media.

🔍 Selective Enrichment Step

Functions as an enrichment stage prior to plating onto selective Listeria agars and further biochemical or confirmatory testing.

✨ Key Features

  • Buffered nutrient formulation supports target recovery
  • Designed for Listeria enrichment workflows
  • Suitable for supplement addition according to method requirements
  • Useful for food and environmental microbiology
  • Supports downstream isolation on selective agar media
  • Available with COA and SDS support

🧪 Typical Composition (g/L)

Component Approx. Role Function
Peptones / digests Primary nutrient source Support growth and recovery of Listeria cells
Yeast extract Growth factor source Provides vitamins and accessory nutrients
Dextrose / carbohydrate source Energy source Supports enrichment and active metabolism
Buffering salts pH stabilization Help maintain suitable enrichment conditions
Selective supplements (added separately) Selectivity enhancement Improve inhibition of competing flora

Exact formulation may vary slightly according to manufacturing standard and intended performance specification.

📊 Related Listeria Media Comparison

Medium Type Main Use Typical Role
Buffered Listeria Enrichment Broth Base Selective enrichment broth base Recovery and enrichment of Listeria spp. Primary enrichment step
Half Fraser Broth Selective enrichment broth Primary Listeria enrichment with selective agents Early enrichment workflow
Fraser Broth Secondary selective enrichment broth Further selective enrichment of Listeria Secondary enrichment stage
Oxford Agar / PALCAM Agar / Chromogenic Listeria Agar Selective differential agar Isolation and colony differentiation Post-enrichment plating

💡 Preparation & Use Guidance

Use Case:
For selective enrichment of Listeria from food and environmental samples
Supplementation:
Add the required selective supplement according to your validated method
Next Step:
Subculture enriched broth onto suitable selective Listeria agar
Storage:
Store dehydrated medium tightly closed in a cool, dry place

📋 Technical Specifications

Product Name Buffered Listeria Enrichment Broth Base
Catalog Number AS-1157
Format Dehydrated powder
Medium Type Selective enrichment broth base
Primary Function Enrichment of Listeria spp.
Typical Supplementation Selective supplement added separately
Appearance Light beige to cream powder
Prepared Medium Amber to pale brown broth
Storage Keep tightly closed in a dry place, protected from moisture and light

🎯 Typical Organism Scope

  • Listeria monocytogenes
  • Other Listeria spp.
  • Stressed or low-level target cells
  • Food and environmental monitoring isolates

✅ Quality & Documentation

  • Microbiological grade raw materials
  • Batch QC documentation
  • COA available
  • SDS support

📄 Available Documents

Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)

🧫 Related AuSaMicS Media

Half Fraser Broth

Primary Listeria enrichment

View Product
Fraser Broth

Secondary selective enrichment

View Product
Oxford / PALCAM / Chromogenic Listeria Agar

Post-enrichment plating media

View Collection
Listeria Workflow Range

Broths and agars for Listeria testing

View Collection

Need a complete Listeria detection workflow?

AuSaMicS can support enrichment broths, selective agars, supplements, and technical documentation for food and environmental Listeria testing.

For laboratory and research use only. Use according to validated laboratory methods and internal quality procedures.

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