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Cetrimide Agar Base (AS-1171) | Pseudomonas aeruginosa Isolation
$45.00 AUD
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AuSaMicS Life Science • Culture Media
Cetrimide Agar Base
Selective medium for isolation of Pseudomonas aeruginosa
Cetrimide Agar Base is a selective culture medium designed for the isolation and presumptive identification of Pseudomonas aeruginosa from clinical, environmental, and industrial samples. Cetrimide suppresses most competing microbiota, while magnesium chloride and potassium sulfate enhance pyocyanin production for clearer presumptive identification.
AS-1171
Selective Medium
✓ Pseudomonas Recovery
🧫 Pyocyanin Support
🦠 Target Organism: P. aeruginosa
💧 Sample Types: water, surfaces, clinical samples
📄 Available: COA + TDS + SDS
🧪 Pseudomonas Specialist Medium
🛡️ Cetrimide Selectivity - inhibits most competing flora
💙 Pyocyanin Enhancement - supports blue-green pigment expression
🔬 Routine Monitoring - suitable for environmental and industrial use
📋 Clear Readout - distinctive colony appearance
📞 Technical Support - workflow guidance available
Characteristic Colony Appearance
Blue-green pigmented colonies typical of pyocyanin-positive strains
🔬 Product Overview
Cetrimide Agar Base is formulated for the selective isolation and presumptive identification of Pseudomonas aeruginosa from mixed microbial populations. It is commonly used where reliable recovery of this organism is needed from clinical specimens, environmental waters, surfaces, and industrial hygiene samples.
The medium uses cetrimide, a quaternary ammonium compound, to inhibit the growth of many non-target organisms by disrupting cell membranes. P. aeruginosa shows natural tolerance under these selective conditions, enabling preferential recovery.
Magnesium chloride and potassium sulfate promote production of pyocyanin, the characteristic blue-green pigment that supports presumptive recognition of typical colonies.
⚗️ Principle of the Medium
Cetrimide Agar Base combines selective inhibition with pigment enhancement for practical presumptive identification:
Cetrimide present in medium
↓
Inhibition of most competing organisms
↓
Selective recovery of P. aeruginosa
Magnesium chloride + potassium sulfate
↓
Enhanced pyocyanin production
↓
Blue-green pigmented colonies
↓
Inhibition of most competing organisms
↓
Selective recovery of P. aeruginosa
Magnesium chloride + potassium sulfate
↓
Enhanced pyocyanin production
↓
Blue-green pigmented colonies
Interpretation note: typical P. aeruginosa colonies may show blue-green pigmentation due to pyocyanin production, while most non-target organisms are inhibited or markedly suppressed.
🧫 Applications
🏥 Clinical Microbiology
Isolation of Pseudomonas aeruginosa from clinical specimens where selective recovery is required.
💧 Water Monitoring
Environmental monitoring of water systems and enumeration of P. aeruginosa in manufacturing or distribution systems.
🏭 Industrial Hygiene
Suitable for environmental and hygiene testing in pharmaceutical, cosmetic, and manufacturing facilities.
🧪 Routine QC
Useful in routine quality control and environmental monitoring programs where P. aeruginosa must be screened selectively.
📊 Key Features & Benefits
Selective Inhibition
Suppresses most Gram-positive and many Gram-negative competing organisms.
Suppresses most Gram-positive and many Gram-negative competing organisms.
Promotes Target Recovery
Supports selective isolation of Pseudomonas aeruginosa.
Supports selective isolation of Pseudomonas aeruginosa.
Pyocyanin Enhancement
Encourages blue-green pigment production for easier presumptive recognition.
Encourages blue-green pigment production for easier presumptive recognition.
Broad Utility
Suitable for clinical, environmental, and industrial microbiology workflows.
Suitable for clinical, environmental, and industrial microbiology workflows.
🧪 Typical Colony Appearance
Pseudomonas aeruginosa
Blue-green pigmented colonies typical of pyocyanin-positive strains.
Blue-green pigmented colonies typical of pyocyanin-positive strains.
Other organisms
Usually inhibited or significantly suppressed under selective conditions.
Usually inhibited or significantly suppressed under selective conditions.
💡 Preparation Guidance
Prepare according to the product label instructions for dehydrated medium. Dissolve in purified water with heating and agitation until completely dissolved, sterilize by autoclaving at 121 °C for 15 minutes, cool to 45–50 °C, and pour into sterile Petri dishes.
📋 Technical Specifications
| Product Name | Cetrimide Agar Base |
| Catalogue Number | AS-1171 |
| Medium Type | Selective agar medium |
| Primary Use | Isolation of P. aeruginosa |
| Selective Agent | Cetrimide |
| Differentiation Aid | Enhanced pyocyanin production |
| Sample Types | Clinical, environmental, industrial |
| Sterilization | Autoclave at 121 °C for 15 min |
| Prepared Plate Storage | 2–8 °C |
| HS / AHECC Code | 3821.00 |
🌡️ Storage & Stability
- ✓ Dehydrated medium: 15–30 °C
- ✓ Keep tightly sealed
- ✓ Prepared plates: 2–8 °C
- ✓ Use within recommended shelf life for optimal performance
✅ Intended Use
- ✓ Laboratory microbiology
- ✓ Environmental monitoring
- ✓ Industrial microbiology use
- ✓ Not for human or veterinary therapeutic use
🏭 Quality & Compliance
Manufactured under controlled conditions to ensure consistent selectivity and performance, and suitable for routine quality control and environmental monitoring programs.
📄 Available Documentation
Technical Data Sheet (TDS)
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
🧬 Related Pseudomonas Testing Products
Complementary Media & Workflow Products
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AuSaMicS Pty Ltd • 31 Longview CT, Thomastown, VIC 3074, Australia • ABN 56676640467
Website: www.ausamics.com.au • Email: support@ausamics.com • Phone: +61 412 520 598
For laboratory, environmental, and industrial microbiology use only. Product suitability should be verified by the user under validated isolation, confirmation, and monitoring procedures.