Clostridium difficile Agar (CCFA) | Selective Medium for C. difficile Isolation | AS‑1177
$155.00 AUD
AuSaMicS Life Science • Anaerobic Selective Media
Clostridium difficile Agar (CCFA)
Selective Cycloserine-Cefoxitin Fructose Agar for Isolation of C. difficile
CCFA is a highly selective medium formulated for the isolation of Clostridium difficile from clinical and environmental samples. The medium combines cycloserine and cefoxitin to suppress competing flora, while fructose fermentation and neutral red indicator enable clear colony differentiation.
AS-1177
C. difficile Selective
Cycloserine + Cefoxitin
✓ Differential
🧫 Strict anaerobe isolation
🏥 Clinical stool testing
🔬 Toxin-producing strains
🏆 C. difficile Standard Medium
🦠 Target: C. difficile
⚗️ High selectivity
📋 COA + SDS available
🔬 Clinical-grade formulation
Key Function
Selective recovery of toxigenic C. difficile
🔬 Principle of the Medium
CCFA contains cycloserine and cefoxitin to inhibit most facultative bacteria and Gram-negative organisms. This creates a highly selective environment favoring Clostridium difficile.
Fructose serves as a fermentable carbohydrate, while neutral red acts as a pH indicator. Fermentation results in acid production and color change, aiding differentiation of colonies.
Cycloserine + Cefoxitin → Inhibit competing flora
↓
Fructose fermentation → Acid → Yellow colonies
↓
Fructose fermentation → Acid → Yellow colonies
🧪 Applications
- Isolation of C. difficile from stool samples
- Hospital and clinical microbiology diagnostics
- Environmental monitoring (healthcare settings)
- Research on toxin-producing strains
📊 Clostridium difficile Media Comparison
| Medium | Selectivity | Differentiation | Best Use |
|---|---|---|---|
| CCFA | High | Fructose + indicator | Clinical isolation |
| Chromogenic C. difficile Agar | Very high | Chromogenic | Rapid identification |
| Cooked Meat Medium | Low | None | Anaerobic enrichment |
| Clostridial Agar | Moderate | Limited | General Clostridium spp. |
💡 Practical Notes
- Requires strict anaerobic incubation
- Use enrichment step for low-level samples
- Typical colonies: yellow, ground-glass appearance
- Characteristic odour may be observed
📋 Technical Specifications
| Product Code | AS-1177 |
| Format | Dehydrated powder |
| Type | Selective differential agar |
| Key Components | Cycloserine, cefoxitin, fructose |
| Final pH | ~7.4 ± 0.2 |
| Storage | 15–25°C dry |
| HS Code | 3821.00.00 |
🧫 Colony Morphology
- 🟡 Yellow colonies (fructose positive)
- ✨ Ground-glass texture
- 🦠 Characteristic odour
✅ Quality
- ✓ High selectivity performance
- ✓ Reliable differentiation
- ✓ COA available
Disclaimer: For laboratory and research use only.