DEV Gelatin Agar | Medium for Gelatin Liquefaction Testing | AS‑1195

$125.00 AUD

AuSaMicS Life Science • Culture Media

DEV Gelatin Agar

Differential Nutrient Agar for Total Count and Detection of Gelatin-Liquefying Microorganisms in Water

DEV Gelatin Agar is a non-selective nutrient medium used for the determination of total microbial count and for the detection of gelatin-liquefying microorganisms, especially in water-testing workflows aligned with classical German DEV methodology.

AS-1195 Gelatinase Detection ✓ Water Testing DEV / DIN Style

💧 Water and wastewater microbiology 🧫 Total count + proteolysis readout 🔬 Classical gelatinase screening medium

🏆 Proteolysis Indicator Medium

💧 Built for water microbiology workflows

🦠 Detects gelatin-liquefying microorganisms

📋 COA + SDS available

🧪 Gelatin acts as nutrient and substrate

🔬 Supports total viable count applications

Key Positioning

Count medium plus gelatinase detection in one plate system

🔬 Principle of the Medium

DEV Gelatin Agar is described as a medium for determining total microbial count and for detecting gelatin-liquefying microorganisms in water. Gelatin in the formulation serves not only as a nutrient source but also as the substrate for gelatinase / proteinase activity. Microorganisms capable of proteolysis digest the gelatin, supporting identification of gelatin-liquefying colonies.

The nutrient base typically includes peptone from meat, meat extract, sodium chloride, gelatin, and agar, providing broad support for non-fastidious bacteria while retaining the classical DEV performance profile.

            Nutrient base + gelatin substrate

            ↓

            Broad growth + detection of gelatinase-positive microorganisms

🧬 Applications

💧 Water Testing

Used for total microbial population testing in water and wastewater, especially in classical DEV-aligned workflows.

🧪 Gelatinase Detection

Suitable for detecting microorganisms that secrete proteolytic enzymes capable of digesting gelatin.

🏭 Food & Environmental Hygiene

Relevant for monitoring hygienic quality where total count and proteolytic flora are both of interest.

🔬 Routine Microbiology

Can also be used as a classical non-selective nutrient medium for broad cultivation of non-fastidious organisms.

📊 Comparative Water Count & Proteolysis Media Guide

Medium	Primary Role	Best Use	Key Advantage
DEV Gelatin Agar	Total count + gelatinase detection	Water and wastewater microbiology	Combines count function with proteolysis readout
DEV Nutrient Agar	Total microbial count	Water total count only	Classical count medium without gelatinase focus
Plate Count Agar	Standard viable count medium	General food and dairy counts	Widely used standard enumeration agar
Nutrient Gelatin	Gelatin liquefaction testing	Biochemical differentiation of isolates	Classical liquefaction test format

💡 Practical Notes

Medium Type:
Non-selective nutrient agar with gelatin substrate

Typical Workflow:
Water count plus gelatin-liquefier screening

Commercial Positioning:
Best for classical DEV-style water labs needing proteolysis information

Useful Distinction:
More specialized than DEV Nutrient Agar because gelatin is part of the detection concept

📋 Technical Specifications

Product	DEV Gelatin Agar
Cat. No.	AS-1195
Format	Dehydrated powder
Medium Type	Non-selective nutrient agar with gelatin substrate
Typical Composition	Peptone from meat 10 g/L, meat extract 10 g/L, sodium chloride 5 g/L, gelatin 10 g/L, agar 15 g/L
Preparation Load	About 50 g/L in some supplier formats
Final pH	7.1–7.5 at 25°C
Prepared Appearance	Yellowish-brown, clear to slightly turbid
Special Property	Liquid after 2 hours at 45°C in Merck spec
Storage	15–25°C or ambient dry storage depending on supplier guidance
HS Code	3821.00.00

🧫 Interpretation & Performance

✓ Suitable for total microbial population testing
✓ Detects gelatin-liquefying / gelatinase-positive microorganisms
✓ Merck QC data lists recovery for E. coli, Proteus hauseri, S. aureus, E. faecalis, P. aeruginosa, and A. hydrophila

✅ Quality

✓ Reproducible classical DEV performance
✓ Count and proteolysis assessment on one medium
✓ COA issued per lot

Disclaimer: For laboratory and research use only. Not for household, food, or therapeutic use.