DEV Glutamate Broth | Nutrient‑Rich Medium for Bacterial Cultivation | AS‑1196
$125.00 AUD
AuSaMicS Life Science • Culture Media
DEV Glutamate Broth
Selective Enrichment Medium for Coliform Detection in Water (DEV / DIN Method)
DEV Glutamate Broth is a selective enrichment medium designed for the detection and recovery of coliform bacteria in water samples. Based on the classical German DEV methodology, the medium uses glutamate as the primary carbon and nitrogen source, favouring coliform growth while suppressing competing flora.
AS-1196
Coliform Enrichment
✓ Water Testing
DEV / DIN Standard
💧 Drinking water microbiology
🧫 Selective coliform enrichment
🔬 Supports stressed organism recovery
🏆 Coliform Detection Medium
🦠 Targets coliform bacteria
⚗️ Glutamate-based selectivity
📋 COA + SDS available
💧 Water analysis ready
Key Function
Selective enrichment of coliforms
🔬 Principle of the Medium
The medium utilizes L-glutamate as the primary nutrient source, which is efficiently metabolized by coliform bacteria. This selective nutrient environment limits the growth of non-target organisms while allowing coliforms to proliferate.
Phosphate buffering maintains a stable pH during bacterial growth, and selective conditions help enhance recovery from low-level or stressed populations typically encountered in treated water systems.
Glutamate metabolism → growth of coliforms
↓
Suppression of competing flora
↓
Suppression of competing flora
🧪 Applications
- Detection of coliform bacteria in drinking water
- Water quality monitoring (potable, processed, and environmental water)
- Enrichment step prior to confirmatory plating
- Routine microbiological control in water systems
📊 Coliform Detection Media Comparison
| Medium | Type | Selectivity | Use |
|---|---|---|---|
| DEV Glutamate Broth | Enrichment | Moderate | Water coliform detection (DEV) |
| Lauryl Sulphate Broth (LSB) | Enrichment | Selective | Presumptive coliform test |
| EC Broth | Selective | High | E. coli confirmation |
| Endo Agar | Differential | Moderate | Coliform enumeration |
💡 Practical Notes
- Designed specifically for water microbiology workflows
- Often used as a pre-enrichment step
- Compatible with downstream confirmatory media
- Alternative to traditional lactose-based enrichment systems
📋 Technical Specifications
| Product Code | AS-1196 |
| Format | Dehydrated powder |
| Type | Selective enrichment broth |
| Key Component | L-glutamate |
| Final pH | ~6.7–7.0 |
| Storage | 15–25°C dry |
| HS Code | 3821.00.00 |
🧫 Expected Results
- ✓ Growth of coliform bacteria
- ✓ Suppression of competing organisms
- ✓ Suitable for further confirmation steps
✅ Quality
- ✓ Reliable enrichment performance
- ✓ Consistent batch quality
- ✓ COA available
Disclaimer: For laboratory and research use only.