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Diluting Fluid K (Buffered Peptone Diluent), Dehydrated | AS-1414 | AuSaMicS Melbourne
$115.00 AUD
Diluting Fluid K (Peptone-Polysorbate Diluent), Dehydrated | AS-1414
Buffered rinsing and dilution medium for sterility testing and microbiological analysis
Diluting Fluid K (AS-1414) is a dehydrated peptone-polysorbate diluent designed for preparation of rinsing and sample dilution fluids used in sterility testing and microbiological examination. The formulation supports effective wetting and recovery of microorganisms from oily, viscous, or difficult-to-filter samples.
Product Overview
| Product | Diluting Fluid K |
| Catalogue Number | AS-1414 |
| Form | Dehydrated powder |
| Prepared Medium | Peptone-polysorbate solution |
| pH (25°C) | 6.9 ± 0.2 |
| Standards | USP <71>, Ph. Eur. 2.6.1, JP equivalent methods |
| Manufacturer | AuSaMicS Life Science, Melbourne |
Applications
- Rinsing fluid for membrane filtration sterility testing
- Recovery of microorganisms from oily or hydrophobic samples
- Method suitability and validation studies
- Rinsing of medical device pathways and components
Why this formulation is used
- Polysorbate system improves wetting of difficult sample matrices
- Supports reliable microbial recovery in sterility testing
- Suitable for challenging formulations including oils and emulsions
- Consistent performance across microbiological applications
Preparation
Suspend 18.0 g of dehydrated medium in 1000 mL of purified water. Heat if necessary to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes or by a validated equivalent method.
Appearance
- Dehydrated medium: Cream to yellow, free-flowing powder
- Prepared medium: Clear to slightly opalescent pale yellow solution
Storage
Store the dehydrated medium at 10–30°C in a dry environment. Keep container tightly closed and protected from moisture and light.
Equivalent Products
- Comparable to Oxoid peptone-based rinsing fluids
- Alternative to BD Difco and Merck sterility testing diluents
- Equivalent to pharmacopeial Fluid K-type formulations
Frequently Asked Questions
What is Diluting Fluid K used for?
Diluting Fluid K is used as a rinsing and dilution fluid in sterility testing, particularly for samples that are oily, viscous, or difficult to wet. It improves microbial recovery during membrane filtration procedures.
Where can Diluting Fluid K be supplied in Australia?
AS-1414 is supplied by AuSaMicS Life Science with dispatch from Melbourne, supporting laboratories and quality control facilities across Australia.
Available pack sizes: 500 g, 2.5 kg, bulk quantities
Dispatch: 24–48 hours from Melbourne
Use: For laboratory and industrial use only
Dispatch: 24–48 hours from Melbourne
Use: For laboratory and industrial use only