Dixon’s Agar, Modified | Selective Medium for Malassezia Species | AS‑1210

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AuSaMicS Life Science • Culture Media

Dixon’s Agar, Modified

Lipid-Enriched Medium for Isolation and Cultivation of Malassezia Species

Dixon’s Agar, Modified is a specialized enriched medium developed for the primary isolation, cultivation, and maintenance of lipophilic yeasts, especially Malassezia furfur and related Malassezia species. Its malt extract base and added lipid components provide the fatty nutritional environment required for recovery of these fastidious skin-associated yeasts.

AS-1210 Malassezia Culture ✓ Lipid-Enriched Clinical Mycology
🧫 Designed for lipophilic yeasts 🧪 Tween 40 + glycerol mono-oleate support 🔬 Useful for skin mycology workflows

🏆 Malassezia Recovery Medium

🦠 Primary isolation of Malassezia
⚗️ Lipid supplementation for fastidious strains
📋 COA + SDS available
🩺 Suitable for dermatology and clinical mycology labs
🔬 Supports maintenance and subculture
Key Advantage
Provides fatty nutritional support absent in routine fungal media

🔬 Principle of the Medium

Malassezia species are lipophilic yeasts commonly associated with sebaceous areas of human and animal skin, so in vitro growth requires media supplemented with natural oils or other fatty substances. Modified Dixon’s Agar addresses this by combining a rich malt extract–peptone base with lipid supplements such as Tween 40 and glycerol mono-oleate. The acidic pH also favors fungal growth while helping suppress contaminating bacteria.

Malt extract + peptone + lipid supplements

Supports growth of lipophilic Malassezia spp.

🧬 Applications

🩺 Clinical Dermatology

Suitable for primary isolation of Malassezia from skin scrapings and other dermatological specimens in clinical mycology workflows.

🔬 Isolation & Maintenance

Useful not only for primary recovery but also for cultivation, maintenance, and subculture of lipophilic yeasts that grow poorly on routine fungal media.

🧫 Comparative Mycology Studies

Appropriate for laboratories comparing growth behavior of Malassezia strains against conventional yeast and mold media.

🐾 Veterinary & Research Use

Suitable for animal mycology and broader research involving warm-blooded host-associated lipophilic yeasts.

📊 Comparative Malassezia Media Guide

Medium Primary Role Best Use Key Advantage
Dixon’s Agar, Modified Enriched lipid-dependent fungal medium Primary isolation of Malassezia Lipid-rich support for fastidious lipophilic yeasts
Sabouraud Dextrose Agar General fungal isolation medium Routine yeasts and molds Broad fungal use, but less suited to strict lipid requirement
Leeming-Notman Agar Specialized lipid-enriched medium Malassezia culture and recovery Alternative specialized medium for lipophilic species
Potato Dextrose Agar General yeast and mold cultivation Routine fungal growth Useful broad medium, not optimized for lipid-dependent yeasts

💡 Practical Notes

Medium Type:
Enriched fungal agar
Key Supplements:
Tween 40 and glycerol mono-oleate
Primary Organism Group:
Lipophilic Malassezia species
Workflow Fit:
Isolation, maintenance, and clinical mycology research

📋 Technical Specifications

Product Dixon’s Agar, Modified
Cat. No. AS-1210
Format Dehydrated powder / lipid-supplemented system
Medium Type Specialized enriched fungal medium
Typical Composition Basis Malt extract, peptone, bile, agar, Tween 40, glycerol mono-oleate
Indicative pH 6.0 ± 0.2 at 25°C
Prepared Appearance Brownish-yellow, opalescent gel
Storage 10–30°C, dry, tightly closed
HS Code 3821.00.00

🧫 Growth Profile

  • ✓ Supports good to luxuriant growth of Malassezia furfur
  • ✓ Also supports several common yeasts in QC data
  • ✓ Best positioned for lipid-dependent yeast recovery

✅ Quality

  • ✓ Reproducible lipid-enriched formulation
  • ✓ Suitable for routine and specialist mycology labs
  • ✓ COA issued per lot

Disclaimer: For laboratory and research use only. Not for household, food, or therapeutic use.

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