Container of AusaMics Enterococcosel Agar with red lid on a gray background

Enterococcosel Agar | Selective Bile‑Esculin Medium for Enterococci | AS‑1221

$155.00 AUD

AuSaMicS Life Science • Culture Media

Enterococcosel Agar

Selective Differential Agar for Rapid Detection and Enumeration of Enterococci

Enterococcosel Agar is a bile esculin azide agar designed for the rapid, selective detection and enumeration of enterococci. The medium combines bile tolerance, sodium azide selectivity, and esculin hydrolysis detection to generate a clear dark brown to black reaction around positive colonies.

AS-1221 Bile Esculin Azide Agar ✓ Enterococcus Selective Blackening Readout

🧪 Esculin + ferric ammonium citrate indicator 🧫 Bile and azide selectivity 💧 Clinical, food, and water microbiology

🏆 Enterococcus Isolation Plate

🦠 Selective for enterococci and Group D streptococci

⚗️ Strong brown-black esculin reaction

📋 COA + SDS available

📈 Suitable for enumeration workflows

🔬 Good follow-up or direct isolation medium

Key Readout

Black halo or darkening around positive colonies

🔬 Principle of the Medium

Enterococcosel Agar is a Bile Esculin Agar with Azide. Enterococci hydrolyse esculin to esculetin and dextrose; esculetin then reacts with ferric citrate or ferric ammonium citrate to form a dark brown to black complex. Oxgall provides bile selectivity, while sodium azide suppresses many Gram-negative organisms, increasing recovery of enterococci and related Group D streptococci. :contentReference[oaicite:0]{index=0}

            Esculin

            ↓ hydrolysis

            Esculetin + dextrose

            ↓ + ferric iron

            Brown-black complex (positive)

🧬 Applications

🏥 Clinical Isolation

Used for rapid selective isolation and presumptive identification of enterococci from clinical specimens.

💧 Water Microbiology

Relevant for enterococcal detection workflows where bile tolerance and esculin hydrolysis are useful screening traits.

🍽️ Food & Environmental Testing

Suitable for detection and enumeration of enterococci in food, environmental, and hygiene-monitoring programs.

🔬 QC & Research

Useful in quality control and research laboratories requiring a reliable selective differential plate for enterococci.

📊 Enterococcus Media Comparison

Medium	Type	Primary Role	Key Feature
Enterococcosel Agar	Selective & differential agar	Rapid detection and enumeration	Bile + azide + esculin blackening
Enterococcosel Broth	Selective & differential broth	Presumptive detection / enrichment	Blackening in liquid format
Bile Esculin Agar	Selective & differential agar	Group D screening	Bile-esculin without azide emphasis
M-Enterococcus Agar	Selective agar	Primary recovery in some workflows	Often paired with confirmation media

💡 Practical Notes

Medium Type:
Selective differential agar

Selectivity:
Oxgall plus sodium azide

Primary Readout:
Blackening from esculin hydrolysis

Workflow Fit:
Isolation, screening, presumptive enumeration

📋 Technical Specifications

Product	Enterococcosel Agar
Cat. No.	AS-1221
Format	Dehydrated powder
Medium Type	Selective differential agar
Core Components	Casein digest, animal tissue digest, yeast extract, oxgall, sodium chloride, esculin, ferric ammonium citrate, sodium azide, sodium citrate, agar
Indicative Formula Load	Approx. 51–56 g/L depending on supplier format
Prepared Appearance	Clear to slightly hazy beige to amber agar
Storage	10–30°C, dry, tightly closed
HS Code	3821.00.00

🧫 Interpretation

✓ Positive: growth with dark brown/black surrounding medium
✓ Selective for enterococci and related bile-tolerant organisms
✓ Useful for rapid presumptive screening and counting

✅ Quality

✓ Clear selective differential performance
✓ Enumeration-friendly plate format
✓ COA issued per lot

Disclaimer: For laboratory and research use only. Not for household, food, or therapeutic use.