Container of AusaMics Enterococcosel Broth with red lid on a gray background

Enterococcosel Broth | Selective Medium for Enterococci Detection | AS‑1222

$150.00 AUD

AuSaMicS Life Science • Culture Media

Enterococcosel Broth

Selective Differential Broth for Detection of Enterococci via Esculin Hydrolysis

Enterococcosel Broth is a selective and differential medium designed for the detection and presumptive identification of Enterococcus species. It combines bile salt selectivity with esculin hydrolysis detection, producing a characteristic blackening reaction in the presence of esculin-positive organisms.

AS-1222 Enterococcus Detection ✓ Esculin Positive Bile Selective

🧪 Esculin + ferric iron indicator system 🧫 Bile inhibition of non-enterococci 🏥 Clinical & water microbiology applications

🏆 Enterococcus Screening Broth

🦠 Selective for Group D streptococci

⚗️ Strong blackening reaction

📋 COA + SDS available

💧 Suitable for environmental testing

🔬 Reliable presumptive identification

Key Reaction

Blackening of broth indicates esculin hydrolysis

🔬 Principle of the Medium

Enterococcosel Broth contains bile salts to inhibit most Gram-positive organisms other than enterococci, and esculin as a differential substrate. Enterococci hydrolyse esculin to esculetin, which reacts with ferric ions to produce a black complex.

The combination of bile tolerance and esculin hydrolysis provides a reliable presumptive identification system for Enterococcus spp. and Group D streptococci.

            Esculin

            ↓ hydrolysis

            Esculetin + dextrose

            ↓ + ferric ions

            Black precipitate (positive)

🧬 Applications

🏥 Clinical Microbiology

Used for presumptive identification of enterococci from clinical specimens including urine, wound, and blood cultures.

💧 Water Testing

Suitable for detection and confirmation of enterococci in environmental and recreational water microbiology workflows.

🍽️ Food Microbiology

Used for monitoring enterococcal contamination in food products and production environments.

🔬 QC & Research

Useful in quality control workflows and research applications requiring reliable detection of bile-tolerant esculin-positive organisms.

📊 Enterococcus Media Comparison

Medium	Type	Role	Key Feature
Enterococcosel Broth	Selective & Differential	Detection & confirmation	Bile + esculin blackening
Enterococcosel Agar	Selective & Differential	Isolation	Solid medium equivalent
Bile Esculin Agar	Selective	Group D identification	Classic bile-esculin test
M-Enterococcus Agar	Selective	Initial recovery	Used before confirmation

💡 Practical Notes

Blackening indicates esculin-positive organisms
Bile ensures selectivity against non-enterococci
Used as presumptive (not final confirmatory) test
Best used alongside selective isolation media

📋 Technical Specifications

Product	Enterococcosel Broth
Cat No	AS-1222
Format	Dehydrated powder
Type	Selective differential broth
Key Components	Bile salts, esculin, ferric citrate
Readout	Blackening reaction
Storage	10–30°C dry
HS Code	3821.00.00

🧫 Interpretation

✓ Positive: Blackened broth
✓ Negative: No color change
✓ Indicates bile-tolerant esculin hydrolysis

✅ Quality

✓ Consistent selectivity
✓ Clear differential reaction
✓ COA per lot

Disclaimer: For laboratory and research use only.