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Eugon Broth | Enrichment Medium for Fastidious & Non-Fastidious Organisms | AS‑1226
$135.00 AUD
Eugon Broth
Enrichment Medium for Fastidious & Non-Fastidious Organisms | Vera (1947) | Cat. No. AS-1226
Cat. No. AS-1226 Eugon Broth pH 7.0 ± 0.2 30.4 g/L L-Cystine 0.7 g/L + Na₂SO₃ 0.2 g/L ✓ BD Difco 246620 Equivalent 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
Cat. No.
AS-1226
Medium
Eugon Broth
pH (25°C)
7.0 ± 0.2
Dissolution
30.4 g/L
Sterilisation
121°C / 15 min
📄 Full DocumentationCOA, TDS & SDS every batch
🇦🇺 Australian StockNo import delays
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportDirect from our team
Overview
Eugon Broth (AS-1226) is a general-purpose enrichment broth formulated by Vera (1947) for luxuriant (eugonic) growth of fastidious and non-fastidious bacteria. The name comes from the Greek eugonos — meaning fertile or productive — reflecting the medium's design goal of maximum growth yield from demanding organisms.
Its triple-peptone base (Proteose Peptone + Trypticase + Soy Peptone) with a reducing system of L-Cystine and Sodium Sulphite supports fastidious aerobes including Neisseria, Haemophilus, Brucella, Francisella, and Lactobacillus. With the addition of blood or Supplement B, it supports even the most demanding clinical pathogens.
Mode of Action
Triple Peptone Base (20 g/L total): Proteose Peptone + Trypticase + Soy Peptone cover a wider amino acid, peptide, and vitamin spectrum than any single peptone — the key reason Eugon Broth outperforms simpler broths for fastidious organisms.
L-Cystine (0.7 g/L): Sulphur amino acid — essential nutrient for Neisseria and Haemophilus; also acts as a reducing agent lowering the ORP of the broth.
Sodium Sulphite (0.2 g/L): Additional O₂ scavenger — together with L-Cystine creates a mildly reducing environment that stimulates fastidious growth without full anaerobiosis.
High Dextrose (5.5 g/L): More than TSB (2.5 g/L) — provides abundant energy for rapid, luxuriant growth.
L-Cystine (0.7 g/L): Sulphur amino acid — essential nutrient for Neisseria and Haemophilus; also acts as a reducing agent lowering the ORP of the broth.
Sodium Sulphite (0.2 g/L): Additional O₂ scavenger — together with L-Cystine creates a mildly reducing environment that stimulates fastidious growth without full anaerobiosis.
High Dextrose (5.5 g/L): More than TSB (2.5 g/L) — provides abundant energy for rapid, luxuriant growth.
Target Organisms
Gram-negative
Neisseria spp.
Requires sulphur source; use with Supplement B
Gram-negative
Haemophilus spp.
Benefits from L-Cystine reducing environment
Gram-negative
Brucella spp.
Slow-growing; Eugon + Supplement B optimal
Gram-negative
Francisella spp.
Highly fastidious; cysteine supplement recommended
Gram-positive
Pasteurella spp.
Animal pathogens; grows well unenriched
Gram-positive
Lactobacillus spp.
Food microbiology; cured meat spoilage
Formula (Vera 1947 / BD Difco 246620 Reference)
| Ingredient | g / L | Function |
|---|---|---|
| Proteose Peptone No. 3 | 7.5 | Long-chain peptides; growth-promoting factors |
| Pancreatic Digest of Casein (Trypticase) | 7.5 | Free amino acids; rich in tryptophan |
| Soy Peptone | 5.0 | Carbohydrates, B-vitamins, accessory growth factors |
| Dextrose (D-Glucose) | 5.5 | Primary carbon and energy source |
| L-Cystine | 0.7 | Sulphur amino acid; reducing agent; stimulates fastidious growth |
| Sodium Chloride (NaCl) | 4.0 | Osmotic balance |
| Sodium Sulphite (Na₂SO₃) | 0.2 | Reducing agent; O₂ scavenger |
| Total | 30.4 g/L | pH 7.0 ± 0.2 at 25°C — Autoclave 121°C / 15 min |
ⓘ A slight white cystine precipitate may be visible in prepared broth — this is normal and does not affect performance.
FAQs
❓ What does "Eugon" mean?
From Greek eugonos meaning "fertile" or "productive." Vera coined "eugonic" to describe the luxuriant growth this medium produces in fastidious organisms that grow poorly in simpler media.
❓ What is Supplement B and when should I use it?
Supplement B (IsoVitaleX equivalent) contains NAD, coenzymes, vitamins. Add aseptically after autoclaving when cultivating Neisseria, Francisella, or Brucella — organisms that require growth factors not in the base formula.
❓ Is AS-1226 equivalent to BD Difco Eugon Broth 246620?
Yes — AS-1226 follows the same Vera (1947) reference formula as BD Difco 246620 and Thermo Scientific/Oxoid R453432. Full COA supplied with every batch.
❓ Why does Eugon Broth have three different peptones?
Each peptone has a different amino acid/peptide profile. Together they cover a broader nutritional range than any single peptone, which is why Eugon Broth supports a wider spectrum of fastidious organisms compared to TSB or BHI.
Cross-Reference / Equivalents
| Manufacturer | Product Name | Cat. No. |
|---|---|---|
| BD Difco / BD Bacto | Eugon Broth | 246620 |
| Thermo Scientific / Oxoid (Remel) | Eugon Broth | R453432 |
| HiMedia | Eugonic Broth | M429 |
| Neogen (Acumedia) | Eugon Broth | 7149A |
Related Products
Product Specifications
| Product Name | Eugon Broth |
| Catalogue Number | AS-1226 |
| Synonyms | Eugonbroth; Eugon Medium; Eugonic Broth |
| Original Reference | Vera HD. J Bacteriol. 1947;54:14 |
| Commercial Equivalents | BD Difco 246620 | Thermo/Oxoid R453432 | HiMedia M429 |
| Dissolution | 30.4 g/L in distilled water |
| Final pH at 25°C | 7.0 ± 0.2 |
| Sterilisation | Autoclave 121°C for 15 minutes |
| Appearance (powder) | Beige, free-flowing homogeneous powder |
| Appearance (prepared) | Light amber, slightly opalescent; cystine precipitate may be visible (normal) |
| Storage (powder) | Below 30°C, dry, protected from light |
| Storage (prepared) | 2–8°C; use within 2 weeks |
| HS Tariff Code | 3821.00.00 |
Quality Control Organisms
| Organism (ATCC) | Inoculum | Conditions | Recovery |
|---|---|---|---|
| Neisseria meningitidis (13090) | ≤100 CFU | 35°C / 5% CO₂ + Supplement B / 48h | Good ✓ |
| Haemophilus influenzae (10211) | ≤100 CFU | 35°C / 5% CO₂ / 48h | Good ✓ |
| Staphylococcus aureus (25923) | ≤100 CFU | 35°C / aerobic / 24h | Good ✓ |
| Escherichia coli (25922) | ≤100 CFU | 35°C / aerobic / 24h | Good ✓ |
Literature & References
| # | Reference |
|---|---|
| 1 | Vera HD. Studies on the cultivation of the gonococcus. J Bacteriol. 1947;54:14. [Original Eugon formulation] |
| 2 | BD Difco & BBL Manual, 2nd ed. Becton Dickinson; 2009. Eugon Broth, Cat. 246620. |
| 3 | Niven CF. Lactobacilli in meat. Food Res. 1951;16:291. [Eugon Broth for Lactobacillus] |
| 4 | ISO 11133:2014. Performance testing of culture media. Geneva: ISO; 2014. |
📄 Full 16-section GHS SDS available — support@ausamics.com.au
Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | NOT classified as hazardous under Australian WHS Regulations 2023. |
| Signal Word | None required |
| Other Hazards | Sodium Sulphite dust: mild respiratory irritant — use P1 respirator when weighing bulk powder. Combustible powder. Sulphite sensitivity possible in asthmatic individuals. |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr) |
Composition
| Component | g/L | CAS | Hazard |
|---|---|---|---|
| Proteose Peptone No. 3 | 7.5 | 73049-73-7 | Not hazardous |
| Pancreatic Digest of Casein | 7.5 | 91079-38-8 | Not hazardous |
| Soy Peptone | 5.0 | 68153-28-6 | Not hazardous |
| Dextrose | 5.5 | 50-99-7 | Not hazardous |
| L-Cystine | 0.7 | 56-89-3 | Not hazardous at use level |
| Sodium Chloride | 4.0 | 7647-14-5 | Not hazardous |
| Sodium Sulphite | 0.2 | 7757-83-7 | Resp. irritant (dust) — not classified at use level |
| PPE | P1 respirator (bulk powder); safety glasses; nitrile gloves; lab coat |
| Waste | Autoclave inoculated broth (121°C / 15 min) before biological waste disposal |
| Transport | Not dangerous goods — ADG, IMDG, IATA |
⚠️ Biosafety: This medium supports Brucella and Francisella which may require BSL-3 containment. Consult your biosafety officer before use with these organisms.
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Beige, free-flowing, homogeneous | Visual |
| Appearance (prepared) | Light amber; cystine precipitate acceptable | Visual |
| pH (prepared, 25°C) | 7.0 ± 0.2 | pH meter |
| Growth promotion — S. aureus | Good growth ≤100 CFU / 35°C / 24h | ISO 11133:2014 |
| Growth promotion — E. coli | Good growth ≤100 CFU / 35°C / 24h | ISO 11133:2014 |
| Batch COA | Available every lot | On request |
Manufactured by AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 | ABN 56 676 640 467
✓ Formulated per Vera (1947) / BD Difco 246620 reference
✓ Batch QC per ISO 11133:2014 | COA, TDS, SDS with every order
✓ Australian stock — same-week dispatch
✓ Formulated per Vera (1947) / BD Difco 246620 reference
✓ Batch QC per ISO 11133:2014 | COA, TDS, SDS with every order
✓ Australian stock — same-week dispatch
⚠️ For laboratory and research use only. Not for food, therapeutic, or human use.