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Tryptic Soy Broth (TSB) – Soybean-Casein Digest Broth
$77.00 AUD
Documentation
Tryptic Soy Broth (TSB)
Soybean-Casein Digest Broth for General Microbial Cultivation, Enrichment & Laboratory Quality Control | Cat. No. AS-1371
Cat. No. AS-1371
30 g/L
pH 7.3 ± 0.2 at 25°C
500 g → 16.7 L
COA · SDS · TDS Available
Catalogue
AS-1371
Preparation
30 g/L
Final pH
7.3 ± 0.2
500 g Yield
Approx. 16.7 L
Sterilisation
121°C / 15 min
Australian Supply
Australian laboratory supplier with local commercial support
COA Available
Batch-specific certificate available for supplied product
SDS & TDS
Safety and technical documentation available
Institutional Supply
Support for laboratories, procurement and bulk requirements
General-Purpose Broth for Broad Microbial Cultivation
Product
Tryptic Soy Broth (TSB)
Catalogue Number
AS-1371
Format
Dehydrated powder
Preparation Rate
30 g/L
Final pH
7.3 ± 0.2 at 25°C
Documentation
COA, SDS and TDS available
Overview
Tryptic Soy Broth (TSB) is a nutrient-rich, general-purpose, non-selective liquid culture medium used for cultivation, enrichment and maintenance of a broad range of microorganisms.
Also known as Tryptone Soya Broth, CASO Broth, Trypticase Soy Broth and Soybean-Casein Digest Broth, TSB combines pancreatic digest of casein and papaic digest of soybean meal with dextrose, sodium chloride and dipotassium phosphate.
The balanced nutrient composition makes TSB suitable for general microbiological cultivation, enrichment procedures, inoculum preparation, research, food and environmental microbiology and laboratory quality-control workflows where a non-selective nutrient broth is required.
How the Formulation Works
Casein Digest
Provides peptides, amino acids and other nitrogenous nutrients supporting microbial growth.
Soybean Meal Digest
Provides complementary nutrients and growth factors within the medium.
Dextrose
Provides a readily available carbohydrate source.
Sodium Chloride
Contributes to osmotic balance in the prepared broth.
Dipotassium Phosphate
Provides buffering capacity to support pH stability.
Typical Laboratory Applications
General Cultivation
Broad non-selective microbial cultivation and routine culture work.
Enrichment
Nutrient-rich broth for microbiological enrichment procedures.
Inoculum Preparation
Preparation and propagation of microbial cultures for subsequent testing.
Food Microbiology
Suitable for relevant general microbiological food laboratory workflows.
Environmental Testing
General cultivation and enrichment within environmental microbiology.
Research & Quality Control
General-purpose nutrient broth for professional laboratory applications.
Prepared-Medium Yield
| Pack Quantity | Preparation Rate | Approximate Prepared Volume |
|---|---|---|
| 100 g | 30 g/L | 3.33 L |
| 250 g | 30 g/L | 8.33 L |
| 500 g | 30 g/L | 16.67 L |
| 1 kg | 30 g/L | 33.33 L |
Yield calculation:
TSB is prepared at 30 g/L. A 500 g pack therefore prepares
approximately 16.7 litres of culture medium.
Cross-Reference & Supplier Evaluation
Changing from another culture-media supplier?
Ausamics can assist laboratories evaluating Tryptic Soy Broth against an existing supplier specification. Provide the manufacturer, product name and catalogue number currently used by your laboratory when requesting a technical or commercial comparison.
Documentation Available
Certificate of Analysis
Batch-specific COA available for product evaluation and procurement.
Safety Data Sheet
Formal SDS available for laboratory safety and procurement requirements.
Technical Data Sheet
Technical product information available for laboratory review.
Frequently Asked Questions
What is Tryptic Soy Broth?
TSB is a general-purpose, non-selective liquid culture medium used for microbial cultivation, enrichment and maintenance.
Is TSB also called Soybean-Casein Digest Broth?
Yes. Other commonly used names include Tryptone Soya Broth, CASO Broth and Trypticase Soy Broth.
How much TSB is used per litre?
Suspend 30 g in 1 litre of purified water.
How much medium does a 500 g pack prepare?
At 30 g/L, a 500 g pack prepares approximately 16.7 litres.
What is the final pH?
The specified final pH is 7.3 ± 0.2 at 25°C.
Does TSB require autoclaving?
Yes. Sterilise the prepared medium at 121°C for 15 minutes.
Can I obtain a COA, SDS or TDS?
Yes. Product documentation including COA, SDS and TDS is available from Ausamics.
Bulk, institutional or procurement enquiry?
Contact Ausamics for formal quotations, product documentation, laboratory evaluation requirements and commercial quantities of Tryptic Soy Broth.
Contact Ausamics for formal quotations, product documentation, laboratory evaluation requirements and commercial quantities of Tryptic Soy Broth.
Technical Data
Product Specifications
| Product Name | Tryptic Soy Broth (TSB) |
|---|---|
| Catalogue Number | AS-1371 |
| Synonyms | Tryptone Soya Broth; CASO Broth; Trypticase Soy Broth; Soybean-Casein Digest Broth |
| Form | Dehydrated powder |
| Medium Type | General-purpose, non-selective broth |
| Preparation Rate | 30 g/L |
| Final pH | 7.3 ± 0.2 at 25°C |
| Sterilisation | Autoclave at 121°C for 15 minutes |
| Prepared Medium Appearance | Clear to slightly opalescent broth |
| 500 g Prepared Yield | Approximately 16.67 L |
| Documentation | COA, SDS and TDS available |
| HS Tariff Code | 3821.00 |
Typical Composition per Litre
| Ingredient | g/L | Function |
|---|---|---|
| Pancreatic digest of casein | 17.0 | Source of peptides, amino acids and nitrogenous nutrients |
| Papaic digest of soybean meal | 3.0 | Complementary nutrient and growth-factor source |
| Sodium chloride | 5.0 | Osmotic balance |
| Dextrose | 2.5 | Carbohydrate source |
| Dipotassium phosphate | 2.5 | Buffering component |
| Total | 30.0 g/L | Final pH 7.3 ± 0.2 at 25°C |
Preparation Protocol
Suspend 30 g of dehydrated medium in 1 L purified water.
Mix thoroughly.
Heat gently with agitation until the powder is completely dissolved.
Dispense into suitable laboratory containers as required.
Sterilise by autoclaving at 121°C for 15 minutes.
Allow to cool before inoculation or laboratory use.
TSB and Tryptic Soy Agar
| Medium | Physical Form | Typical Role |
|---|---|---|
| Tryptic Soy Broth (TSB) | Liquid medium | Cultivation, enrichment and inoculum preparation |
| Tryptic Soy Agar (TSA) | Solid agar medium | General-purpose cultivation and isolation on a solid surface |
Supplier comparison:
Laboratories switching from another supplier can provide their current
product name and catalogue number to Ausamics for formulation and
purchasing evaluation.
Safety Data
The following provides a concise website summary only. Consult the current Ausamics Safety Data Sheet for complete product safety information.
Identification
| Product | Tryptic Soy Broth (TSB) |
|---|---|
| Catalogue Number | AS-1371 |
| Product Form | Dehydrated microbiological culture medium |
| Recommended Use | Laboratory microbiological culture medium |
Hazard & Handling Summary
The dehydrated product is not generally classified as hazardous under typical GHS criteria for this product form. Avoid unnecessary generation or inhalation of dust and follow normal laboratory handling practices.
- Avoid unnecessary dust generation.
- Avoid inhalation of airborne powder.
- Use appropriate laboratory protective clothing.
- Use gloves and eye protection where appropriate.
- Keep the container tightly closed when not in use.
- Protect the dehydrated medium from moisture.
First-Aid Summary
| Inhalation | Move to fresh air if dust has been inhaled. |
|---|---|
| Skin Contact | Wash with soap and water. |
| Eye Contact | Rinse cautiously with water for several minutes. |
| Ingestion | Rinse mouth. Seek medical advice if unwell. |
Spill & Disposal
Collect spilled dehydrated material carefully while minimising dust formation. Dispose of unused material and containers in accordance with applicable requirements.
Once inoculated, prepared culture medium should be handled and disposed of according to the biological risk associated with the microorganisms involved and the laboratory's approved microbiological waste procedures.
Safety Data Sheet available:
Consult the current formal Ausamics SDS for complete safety,
handling, first-aid, storage and disposal information.
Quality & COA
Ausamics provides product documentation to support laboratory evaluation, procurement and quality review. Batch-specific information should be taken from documentation applicable to the supplied lot.
Product Quality Specifications
| Parameter | Specification |
|---|---|
| Product | Tryptic Soy Broth (TSB) |
| Catalogue Number | AS-1371 |
| Product Form | Dehydrated powder |
| Prepared Medium | Clear to slightly opalescent broth |
| Preparation Rate | 30 g/L |
| pH at 25°C | 7.3 ± 0.2 |
| Sterilisation | 121°C for 15 minutes |
Documentation for Procurement & Quality Review
COA Available
Batch-specific Certificate of Analysis available on request
or with supply as applicable.
SDS Available
Formal safety documentation available for compliance,
procurement and safe handling.
TDS Available
Technical product information available for laboratory,
validation and QC review.
Batch-specific information:
Refer to the applicable COA for batch number, manufacture or
expiry information and any lot-specific test results provided
for the supplied batch.
Laboratory Evaluation
Laboratories incorporating TSB into validated methods or quality systems should confirm that the formulation, preparation method, documentation and performance requirements are suitable for their specific application.
Evaluating an alternative supplier?
Contact Ausamics with your current TSB manufacturer, catalogue number and required documentation. We can assist with technical evaluation, commercial quotation and institutional purchasing requirements.
Contact Ausamics with your current TSB manufacturer, catalogue number and required documentation. We can assist with technical evaluation, commercial quotation and institutional purchasing requirements.