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Fluid Thioglycollate Medium (FTM) | USP <71> Sterility Testing Medium for Aerobic & Anaerobic Bacteria
$65.00 AUD
Fluid Thioglycollate Medium (FTM)
Multi-Purpose Enriched Reducing Medium for Aerobic and Anaerobic Cultivation
Catalogue No: AS-1233
Multi-Purpose Enriched Reducing Medium for Aerobic and Anaerobic Cultivation
Catalogue No: AS-1233
USP <71>
BP 2023
EP 2.6.1
FDA-BAM
ISO 21149
Anaerobic Culture
Australian Made
Overview
Fluid Thioglycollate Medium (FTM) is a multi-purpose enriched reducing liquid medium for the simultaneous cultivation of obligate aerobes, microaerophiles, facultative anaerobes, and obligate anaerobes. Sodium thioglycollate and L-cystine create a self-generating oxygen gradient — aerobic at the surface, anaerobic at the bottom — without requiring anaerobic jars.
Resazurin provides visual monitoring of oxygen penetration (pink = aerobic; colourless = anaerobic). The semi-solid agar (0.75 g/L) maintains fluid consistency for easy pipetting while retarding convection to preserve the gradient. Validated per USP <71>, BP 2023, EP 2.6.1, FDA-BAM, APHA, and ISO 21149.
FTM vs Brewer Modified — Key Differences
| Parameter | FTM (AS-1233) | Brewer Modified (AS-1364) |
|---|---|---|
| Dextrose | 5.5 g/L | 2.5 g/L |
| Agar (semi-solid) | 0.75 g/L | 0.5 g/L |
| NaCl | 2.5 g/L | 5.0 g/L |
| Pharmacopoeial standard | USP <71>, BP, EP 2.6.1 | USP <71>, BP, EP 2.6.1 |
| Best use | Pharmaceutical sterility testing; clinical blood cultures | Routine anaerobic enrichment; Brewer protocols |
Typical Composition (per litre)
| Ingredient | Amount (g/L) | Function |
|---|---|---|
| Pancreatic Digest of Casein | 15.0 | Nitrogen, peptides, amino acids |
| Yeast Extract | 5.0 | Vitamins, growth factors |
| Dextrose (D-Glucose) | 5.5 | Carbon/energy source, mild reductant |
| L-Cystine | 0.5 | Secondary reducing agent, sulfur source |
| Sodium Chloride | 2.5 | Osmotic balance |
| Sodium Thioglycollate | 0.5 | Primary reducing agent — O₂ scavenger |
| Agar (semi-solid) | 0.75 | Oxygen gradient maintenance |
| Resazurin (sodium salt) | 0.001 | Redox indicator |
| Final pH (25 °C) | 7.1 ± 0.2 | |
Applications
- Pharmaceutical sterility testing: USP <71>, BP 2023, EP 2.6.1 — parenterals, ophthalmics, biologics, medical devices
- Clinical microbiology: Blood culture enrichment, anaerobe isolation from wounds/abscesses (C. perfringens, B. fragilis)
- Food & cosmetics: Total viable count — ISO 21149 (cosmetics), APHA (dairy, food), FDA-BAM
- Environmental: Anaerobe screening from soil, sediment, water samples
Preparation
- Suspend 29.25 g in 1 litre of purified water. Heat with agitation to near boiling until dissolved. Do not overheat.
- Dispense into tubes or bottles (10–20 mL per tube for sterility testing).
- Autoclave at 121 °C for 15 minutes with caps loosened.
- Cool to ~25 °C and tighten caps.
- If >30% of medium turns pink: reheat in boiling water bath for 4 minutes. Cool rapidly. Do this once only.
- Prepared medium: light straw-coloured, clear, fluid, without precipitate.
CRITICAL — Pharmaceutical sterility testing: Use only medium that is colourless or at most faintly pink (≤30% depth). Discard if >30% pink. Follow full pharmacopoeial preparation and validation requirements (USP <71>, BP, EP 2.6.1).
Growth Pattern Interpretation
| Observation | Organism Type | Examples |
|---|---|---|
| Turbidity throughout | Facultative anaerobes | Staphylococcus, Enterobacteriaceae |
| Turbidity at bottom only | Obligate anaerobes | Clostridium, Bacteroides, Fusobacterium |
| Pellicle or surface turbidity | Obligate aerobes | Pseudomonas, Bacillus |
| Mid-zone turbidity | Microaerophiles | Campylobacter, Helicobacter |
| No growth after 14 days (pharma) | Sterile — PASS | Document per pharmacopoeia |
| Any growth after 14 days (pharma) | Not sterile — FAIL | Investigate and report |
Storage
| Dehydrated powder | 10–30 °C, tightly sealed, dry, protected from light and humidity |
| Shelf life (powder) | 3 years from manufacture date |
| Prepared broth | 15–30 °C, sealed containers, use within 4 weeks |
| If refrigerated | 2–8 °C — warm to room temperature before use |
| Discard if | Pink >30%, turbid before inoculation, precipitate, or evaporated |
For Laboratory Use Only. Not for human, veterinary, food, or pharmaceutical consumption. Handle inoculated medium at BSL-2. Autoclave all waste before disposal.
For laboratory use only. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Identification
| Product Name | Fluid Thioglycollate Medium (FTM) |
| Catalogue Number | AS-1233 |
| HS Code | 3821.00.00 |
| Pharmacopoeial Equivalents | USP <71>, BP 2023, EP 2.6.1 — Fluid Thioglycollate Medium |
| Regulatory Compliance | FDA-BAM, APHA, ISO 21149 |
| Also known as | FTM, Thioglycollate Broth, Fluid Thioglycollate Broth, THIO |
| Related product | Thioglycollate Medium Brewer Modified (AS-1364) |
Physical & Chemical Specifications
| pH (prepared, 25 °C) | 7.1 ± 0.2 |
| Moisture (loss on drying) | ≤ 5.0% |
| Agar concentration | 0.75 g/L (semi-solid — fluid, not gel) |
| Dextrose concentration | 5.5 g/L |
| Resazurin indicator | 0.001 g/L — colourless (reduced) ↔ pink (oxidised) |
| Reducing system | Dual: sodium thioglycollate (0.5 g/L) + L-cystine (0.5 g/L) |
| Total solids | ~29.25 g/L |
Performance Specifications (Pharmacopoeial)
| Organism | ATCC No. | Expected Result | Ref. |
|---|---|---|---|
| Clostridium sporogenes | 11437 | Growth — lower zone turbidity | USP <71> / EP 2.6.1 |
| Staphylococcus aureus | 6538 | Growth throughout | USP <71> / EP 2.6.1 |
| Pseudomonas aeruginosa | 9027 | Growth at surface | USP <71> / EP 2.6.1 |
| Bacillus subtilis | 6633 | Growth at/near surface | USP <71> / EP 2.6.1 |
| Candida albicans | 10231 | Growth throughout (20–25 °C) | USP <71> |
| Aspergillus brasiliensis | 16404 | Surface growth / pellicle (20–25 °C) | USP <71> |
| Bacteroides fragilis | 25285 | Growth — lower zone | EP / Clinical |
| Clostridium perfringens | 13124 | Growth — lower zone | FDA-BAM / EP |
Key Limitations
- Pink zone must not exceed 30% of tube depth before inoculation
- For pharmacopoeial sterility testing — prepare and validate per applicable pharmacopoeia
- Do not overheat — degrades reducing agents and dextrose
- Reheat only once if pink >30% — repeated heating degrades medium
- Not suitable for strict aerobe isolation without surface inoculation technique
Version 1.0 — March 2026. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
GHS Classification: Mixture not classified as hazardous at use concentration (AU WHS Regulations 2011) | Note: Sodium thioglycollate — H302/H311/H331 at high concentration; negligible risk at 0.5 g/L working level | ADG: Not regulated | BSL-2 precautions for inoculated medium.
Section 1 — Identification
| Product Name | Fluid Thioglycollate Medium (FTM) |
| Catalogue Number | AS-1233 |
| Intended Use | Multi-purpose enriched reducing medium — aerobic/anaerobic cultivation and pharmaceutical sterility testing. Laboratory use only. |
| Supplier | AuSaMicS Pty Ltd | 31 Longview CT, Thomastown VIC 3074 |
| ABN | 56 676 640 467 |
| Emergency Phone | +61 412 520 598 | Poisons Information: 13 11 26 (24 hr) |
| support@ausamics.com.au |
Section 3 — Composition
| Component | CAS | % | Classification |
|---|---|---|---|
| Pancreatic Digest of Casein | 73049-73-7 | ~51.3% | Not classified |
| Yeast Extract | 8013-01-2 | ~17.1% | Not classified |
| Dextrose | 50-99-7 | ~18.8% | Not classified |
| L-Cystine | 56-89-3 | ~1.7% | Not classified |
| Sodium Chloride | 7647-14-5 | ~8.5% | Not classified |
| Sodium Thioglycollate | 367-51-1 | ~1.7% | H302, H311, H331 (at high conc.) |
| Agar | 9002-18-0 | ~2.6% | Not classified |
| Resazurin | 62758-13-8 | <0.01% | Not classified at this level |
Sections 4–8 — First Aid, Handling & PPE
| Topic | Guidance |
|---|---|
| Inhalation | Remove to fresh air. Seek medical advice if symptoms persist. |
| Skin Contact | Wash with soap and water ≥15 minutes. Sodium thioglycollate — potential sensitiser with repeated exposure. |
| Eye Contact | Flush with copious water ≥15 minutes. Seek medical attention. |
| Ingestion | Do not induce vomiting. Rinse mouth. Seek medical advice. Inform physician of sodium thioglycollate content. |
| Inoculated spill | BSL-2. Universal Precautions. Disinfect with 1% sodium hypochlorite. Autoclave all contaminated material. |
| PPE | Nitrile gloves, safety glasses/goggles, lab coat. BSC for clinical/inoculated work. |
Section 13 — Disposal
Uninoculated: Chemical waste per local EPA regulations. Neutralise reducing agents before small-quantity drain disposal.
Inoculated / clinical medium: Autoclave at 121 °C for 30 minutes minimum. Treat as BSL-2 biological waste per institutional and local biosafety requirements.
Section 15 — Regulatory
| WHS Regulations 2011 | Mixture not classified as hazardous at use concentration |
| Pharmacopoeial use | USP <71>, BP 2023, EP 2.6.1 validated medium |
| Transport (ADG/IATA) | Not classified as dangerous goods |
Prepared per Australian WHS Regulations and GHS 7th Edition. Version 1.0 — March 2026. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Information
| Product Name | Fluid Thioglycollate Medium (FTM) |
| Catalogue Number | AS-1233 |
| Pharmacopoeial Ref. | USP <71>, BP 2023, EP 2.6.1 | FDA-BAM | ISO 21149 |
| Lot / Batch Number | Contact us for batch-specific COA |
| Manufacturing Date | Available on request |
| Expiry Date | 3 years from manufacture |
| Storage Conditions | 10–30 °C, tightly sealed, dry; prepared: 15–30 °C, use within 4 weeks |
Specification & Test Results
| Test | Specification | Typical Result | Status |
|---|---|---|---|
| Appearance (powder) | Cream to light beige powder | Complies | ✓ PASS |
| Colour (prepared) | Light straw/clear; pink ≤30% surface | Complies | ✓ PASS |
| pH (prepared, 25 °C) | 7.1 ± 0.2 | Complies | ✓ PASS |
| Moisture (loss on drying) | ≤ 5.0% | Complies | ✓ PASS |
| Solubility | Completely soluble — no precipitate | Complies | ✓ PASS |
| Sterility (prepared) | No microbial growth | Complies | ✓ PASS |
| Resazurin indicator | Colourless (reduced) / pink (oxidised) | Complies | ✓ PASS |
| C. sporogenes ATCC 11437 | Growth — lower zone | Complies | ✓ PASS |
| S. aureus ATCC 6538 | Growth throughout | Complies | ✓ PASS |
| P. aeruginosa ATCC 9027 | Growth at surface | Complies | ✓ PASS |
| C. albicans ATCC 10231 | Growth throughout (20–25 °C) | Complies | ✓ PASS |
| A. brasiliensis ATCC 16404 | Surface growth / pellicle | Complies | ✓ PASS |
| Oxidation limit | Pink zone ≤30% of total depth | Complies | ✓ PASS |
Authorisation
| Released by | Hassan Salimi |
| Title | Founder and Technical Director |
| Organisation | AuSaMicS Pty Ltd |
Need a batch-specific COA?
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
Contact us with your order number and we will issue a full COA within 1 business day.
support@ausamics.com.au | +61 412 520 598
This COA is valid for the batch identified above. Not transferable to other batches. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au