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Fraser Listeria Selective Enrichment Broth (Base) | ISO 11290 Compliant
$95.00 AUD
AuSaMicS Life Science • Pathogen Surveillance
Fraser Listeria Selective Enrichment Broth (Base)
Secondary Enrichment & Detection of Listeria
A specialized selective enrichment medium for the detection of Listeria monocytogenes in food and environmental samples. Formulated to provide rapid growth while inhibiting competing microflora through a high-salt, multi-antibiotic matrix.
AS-1235
🧪 ISO 11290-1 Compliant
✓ Esculin Indicator
🔬 High Selectivity
Reaction: Iron-Esculin Blackening Incubation: 37°C / 24-48h Prep: 54.9g/L Yield
Listeria Specialist
Visual Detection - Rapid blackening response
Dual Enrichment - Supports Half & Full Fraser
Broad Suppression - Inhibits Enterococci & Bacilli
ISO Standard - Validated for global exports
Modified Fraser Formula
Primary Food Safety Standard
🧪 Formulation & Selective Profile
📋 Formulation (per Liter)
| Component | Concentration | Role |
|---|---|---|
| Proteose Peptone / Casein | 10.0 g | Nitrogen & Vitamin source |
| Yeast Extract | 5.0 g | Essential growth factors |
| Sodium Chloride | 20.0 g | High osmotic selectivity |
| Lithium Chloride | 3.0 g | Suppression of Enterococci |
| Esculin / Ferric Ammonium Citrate | 1.5 g | Presumptive blackening system |
Diagnostic Workflow
Blackening Response
Listeria hydrolyze Esculin to Esculetin, which reacts with iron to form a black complex.
Flexible Enrichment
Use as Half-Fraser (primary) or Full-Fraser (secondary) by adjusting selective antibiotic concentration.
📋 Technical Profile
| Catalogue No. | AS-1235 |
| Final pH | 7.2 ± 0.2 |
| Preparation | 54.9g per Liter |
| Compliance | ISO 11290-1 |
⚗️ Laboratory Prep
- 1 Suspend 54.9g in 1L deionized water.
- 2 Autoclave at 121°C for 15 min.
- 3 Cool and add Fraser Selective Supplements before use.