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  • a container of Ausamics Giolitti Cantoni Broth Base with a red lid and gray background
Giolitti Cantoni Broth Base | Enrichment Medium for Staphylococcus aureus Detection | AS‑1238

Giolitti Cantoni Broth Base | Enrichment Medium for Staphylococcus aureus Detection | AS‑1238

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AuSaMicS Life Science • Staphylococcus Enrichment Media

Giolitti Cantoni Broth Base

Selective Enrichment Broth Base for Staphylococcus aureus Detection

Giolitti Cantoni Broth Base is a selective enrichment medium base designed for the detection, enrichment, and MPN-style enumeration of coagulase-positive staphylococci, particularly Staphylococcus aureus, from foods and related samples. In standard use, the sterile broth base is supplemented with potassium tellurite after sterilisation. :contentReference[oaicite:1]{index=1}

AS-1238 pH 6.9 ± 0.2 ✓ Australian Stock 🧫 Staph Enrichment 🍖 Food Microbiology
Target: coagulase-positive staphylococci Standards: ISO 6888-3 aligned Documents: COA + SDS + TDS

 Why Labs Use Giolitti-Cantoni

Selective enrichment for Staph
Tellurite-supplemented workflow
Supports low-number detection
Food microbiology ready
ISO-style MPN use
Basic Workflow
Prepare broth base
Add tellurite after sterilisation
Inoculate sample
Enrich and confirm

⚠️ Important: This is a Broth Base — Potassium Tellurite Is Added Separately

Giolitti Cantoni Broth Base (AS-1238)
The basal medium provides the nutrient and selective foundation for enrichment of coagulase-positive staphylococci. Published directions specify adding 1% potassium tellurite solution after sterilisation and cooling. :contentReference[oaicite:2]{index=2}
Complete working enrichment system
Routine use generally involves the broth base plus tellurite supplement, with method-specific additions or overlays where applicable in the validated protocol. :contentReference[oaicite:3]{index=3}

🔬 Technical Overview & Principle

Giolitti-Cantoni Broth was proposed for the selective enrichment and MPN-based detection of staphylococci from foodstuffs. Current technical sheets describe the broth base as aligned with ISO 6888-3 and ISO 11133 performance criteria, and position it specifically for Staphylococcus aureus recovery from suspected food samples. :contentReference[oaicite:4]{index=4}

Growth stimulants
Published descriptions state that mannitol and sodium pyruvate stimulate the growth of S. aureus and help in detection of small numbers of organisms. :contentReference[oaicite:5]{index=5}
Selective inhibition system
Published technical sheets note that lithium chloride, glycine, and potassium tellurite help suppress competing organisms, while an anaerobic paraffin overlay can inhibit micrococci in some workflows. :contentReference[oaicite:6]{index=6}
Suspected food / sample matrix
↓
Giolitti Cantoni Broth Base + tellurite
↓
Selective enrichment of coagulase-positive staphylococci
↓
MPN / follow-up plating / confirmation
Best positioning: a premium, standards-aligned enrichment broth base for S. aureus and other coagulase-positive staphylococci in food microbiology workflows. :contentReference[oaicite:7]{index=7}

🧪 Functional Composition

Component Group Function Performance Contribution
Peptones / extract base Carbon, nitrogen, vitamins, minerals Published sheets describe peptones and extracts as the main nutrient source supporting enrichment of target staphylococci. :contentReference[oaicite:8]{index=8}
Mannitol Growth stimulant Reported to stimulate S. aureus and aid low-level recovery. :contentReference[oaicite:9]{index=9}
Sodium Pyruvate Growth stimulant / recovery aid Works with mannitol as a stimulant for S. aureus enrichment. :contentReference[oaicite:10]{index=10}
Lithium Chloride Selective inhibitor Reported to inhibit gram-negative lactose-fermenting bacilli. :contentReference[oaicite:11]{index=11}
Glycine Selective pressure component Described together with tellurite as helping inhibit competing gram-positive bacilli. :contentReference[oaicite:12]{index=12}
Potassium Tellurite Post-sterilisation selective supplement Added aseptically after sterilisation according to published directions; a core part of the working selective enrichment system. :contentReference[oaicite:13]{index=13}
Finished medium Published directions list about 55.2 g/L for the broth base, with final pH after sterilisation around 6.9 ± 0.2. :contentReference[oaicite:14]{index=14}

🧫 Typical Laboratory Workflow

1
Prepare the broth base
Published instructions specify suspending approximately 55.2 g in 1 L water, dispensing into tubes, and sterilising at 121°C for 15 minutes. :contentReference[oaicite:15]{index=15}
2
Cool and add tellurite
After rapid cooling, add 1% potassium tellurite solution aseptically to each tube according to the validated method. :contentReference[oaicite:16]{index=16}
3
Inoculate and enrich
Use as part of the validated enrichment or MPN workflow for suspected food or environmental samples related to food production. :contentReference[oaicite:17]{index=17}
4
Confirm presumptive positives
Follow enrichment with the appropriate downstream plating and confirmation steps required by the method. :contentReference[oaicite:18]{index=18}

📊 Comparative Staphylococcus Workflow Media

Medium Best Use Advantages Considerations
Giolitti Cantoni Broth Base Selective enrichment / MPN detection of coagulase-positive staphylococci from foods Standards-aligned enrichment system with strong food-microbiology positioning. :contentReference[oaicite:19]{index=19} Requires tellurite supplementation and downstream confirmation. :contentReference[oaicite:20]{index=20}
Baird-Parker Agar Isolation and enumeration after enrichment Common downstream selective agar in ISO food workflows. :contentReference[oaicite:21]{index=21} Used later in the workflow rather than as the primary enrichment broth.
K.R.A.N.E.P. Agar Selective isolation / enumeration of staphylococci from meat products Alternative selective agar system for staphylococcal workflows. :contentReference[oaicite:22]{index=22} Different role and matrix emphasis compared with Giolitti-Cantoni enrichment broth.
Modified Giolitti-Cantoni variants Method-specific ISO detection workflows Used in some standardised detection and enumeration protocols. :contentReference[oaicite:23]{index=23} Formulation and workflow details vary by protocol and supplier.

📌 Quick Specifications

Product: Giolitti Cantoni Broth Base
Cat. No.: AS-1238
Format: Dehydrated culture medium base
Type: Selective enrichment broth base
Main targets: coagulase-positive staphylococci
Key organism: Staphylococcus aureus
Supplement: potassium tellurite required
Storage: 10–25°C, dry, tightly closed, protected from moisture and light

⭐ Key Advantages

✓Selective enrichment for foodborne staphylococci workflows
✓Supports low-level recovery of S. aureus
✓Well suited to ISO-style MPN and confirmation systems
✓Useful in food and environmental monitoring linked to food production
✓Strong classical positioning for premium technical sales

⚙️ Preparation Notes

Published directions specify about 55.2 g/L, dispensing into tubes, then sterilising at 121°C for 15 minutes. :contentReference[oaicite:24]{index=24}

After rapid cooling, add potassium tellurite aseptically according to the validated method. :contentReference[oaicite:25]{index=25}

Use only within your validated food microbiology protocol and follow with the appropriate downstream plating and confirmation steps. :contentReference[oaicite:26]{index=26}

🔗 Related AuSaMicS Products

Baird-Parker Agar

Selective agar commonly used after enrichment for presumptive staphylococcal isolation

Potassium Tellurite Supplement

Required additive for preparing the complete selective enrichment system

Other Staphylococcus media

Complementary isolation and confirmation media for food microbiology workflows

Standards-Aligned Staphylococcus Enrichment for Food Microbiology

AuSaMicS supplies high-quality microbiological media designed for dependable performance in food, clinical, and industrial laboratories. Contact us for technical documentation, bulk supply, or workflow-aligned support.

For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
www.ausamics.com.au • support@ausamics.com • +61 412 520 598

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